Children Born After Intracytoplasmic Sperm Injection Compared with Spontaneously Conceived Children a Prospective Study
Journal of Gynecology and Obstetrics
Volume 3, Issue 6, November 2015, Pages: 115-120
Received: Oct. 8, 2015; Accepted: Oct. 19, 2015; Published: Dec. 30, 2015
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Moez Kdous, Department of Reproductive Medecine, Aziza Othmana Hospital, Medicine School of Tunis, University of Tunis El Manar, Tunis, Tunisia
Jad Diari, Department of Reproductive Medecine, Aziza Othmana Hospital, Medicine School of Tunis, University of Tunis El Manar, Tunis, Tunisia
Fethi Zhioua, Department of Reproductive Medecine, Aziza Othmana Hospital, Medicine School of Tunis, University of Tunis El Manar, Tunis, Tunisia
Amel Zhioua, Department of Reproductive Medecine, Aziza Othmana Hospital, Medicine School of Tunis, University of Tunis El Manar, Tunis, Tunisia
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Objective: To study the outcome of ICSI conceived children in our IVF center; their growth, their psychomotor development and to find out possible birth defects and genetic anomalies. Material and Methods: This is a prospective survey about ICSI conceived children and naturally conceived children in our IVF center over the period between 2007 and 2012. One hundred ICSI children aged between 2 and 6 years were invited in order to get a thorough physical examination and to undergo ultrasound imaging and genetic explorations for birth defects. One hundred naturally conceived children paired by age and sexduring the same period were taken as a control population. Results: The comparison of both ICSI and natural conception groups showed that the rate of prematurity was higher in the ICSI group with 50% vs 6% in the natural conception group, p<0.05.The rate of low birth weight was also higher in the ICSI group 35% vs 4% in the natural conception group, p<0,05. In the ICSI group, no child had slow growth or slow psychomotor development. 4% of the major birth defects were found in the ICSI group and 2% in the control group, the difference was not significant (p=0.52). These defects were essentially urogenital, orthopedic and facial. In both groups, the affected children were boys. The rate of minor birth defects found in the ICSI children is significantly higher than that in control group (23% vs 4%; p<0.05). These malformations were mainly facial, cardiac, gastrointestinal, orthopedic, neurological, urogenital and inguinal hernia type. In the ICSI group, there was a male predominance (sex ratio=1.3) without any significant difference, while in the control group there was no sex predominance. As for genetic anomalies, 3% of balanced genetic defects were detected in the ICSI children, 1% of which were in the sexual chromosomes and 2% in the autosomes. These anomalies were found in 2 boys and one girl without any significant difference. Conclusion: This work is greatly reassuring about the future of ICSI children. Larger series with long term follow-up are needed as an only guarantee in assessing the safety of ICSI technique.
Child, Congenital Malformation, Follow-Up, Genetic Anomalies, ICSI
To cite this article
Moez Kdous, Jad Diari, Fethi Zhioua, Amel Zhioua, Children Born After Intracytoplasmic Sperm Injection Compared with Spontaneously Conceived Children a Prospective Study, Journal of Gynecology and Obstetrics. Vol. 3, No. 6, 2015, pp. 115-120. doi: 10.11648/j.jgo.20150306.13
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Ludwig M, Katalinic A, for the German ICSI Follow-up Study Group. Malformation rate in fetuses and children conceived after ICSI: results of a prospective cohort study. Reproductive BioMedicine Online, Volume 5, Issue 2, 2002, Pages 171-78.
Palermo GD, Neri Q, Takeuchi T, Squires J, Moy F, Rosenwaks Z. Genetic and epigenetic characteristics of ICSI children. Reprod BioMed Online 2008; 17(6): 820-33.
Bsatemur E, Shevlin M, Sutcliffe A. Growth of children conceived by IVF and ICSI up to 12 years of age. Reprod Bio Med Online 2010 Juan; 20(1): 144-49.
Hansen M, Kurinczuk JJ, Bower C, Webb S. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. New England J Med. 2002; 346: 725-30.
Bonduelle M, Wennerholm UB, Loft A, Tarlatzis BC, Peters C, Henriet S, et al. A multi-centre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Hum Reprod. 2005; 20: 413-9.
Ludwig A1, Katalinic A, Thyen U, Sutcliffe AG, Diedrich K, Ludwig M.. Neuromotor development and mental health at 5.5 years of age of singletons born at term after intracytoplasmatic sperm injection ICSI: results of a prospective controlled single-blinded study in Germany. Fertil Steril. 2009; 91: 125-32.
Ponjaert-Kristoffersen I, Bonduelle M, Barnes J. International Collaborative Study of Intracytoplasmic Sperm Injection-Conceived, In Vitro Fertilization-Conceived, and Naturally Conceived 5-Year-Old Child Outcomes: Cognitive and Motor Assessments. Pediatrics.2005; 115: 283-9.
Ponjaert-Kristoffersen I, Tjus T, Nekkebroeck J, Squires J, Verté D, Heimann M et al. Psychological follow-up study of 5-year-old ICSI children. Hum Reprod. 2004; 19:2791-7.
Leunens L, Celestin-Westreich S, Bonduelle M, Liebaers I, Ponjaert-Kristoffersen I. Cognitive and motor development of 8-year-old children born after ICSI compared to spontaneously conceived children. Hum Reprod. 2006; 21: 2922-9.
Leunens L, Celestin-Westreich S, Bonduelle M, Liebaers I, Ponjaert-Kristoffersen I. Follow-up of cognitive and motor development of 10-year-old singleton children born after ICSI compared with spontaneously conceived children. Hum Reprod. 2008; 23: 105-11.
Palermo GD1, Colombero LT, Schattman GL, Davis OK, Rosenwaks Z. Evolution of pregnancies and initial follow-up of newborns delivered after intracytoplasmic sperm injection. Jama. 1996; 276: 1893-7.
Bonduelle M, Liebaers I, Deketelaere V, et al. Neonatal data on a cohort of 2889 infants born after ICSI (1991-1999) and of 2995 infants born after IVF (1983-1999). Hum Reprod. 2002; 17: 671-94.
Massaro PA, MacLellan DL, Anderson PA, Romao RL. Does intracytoplasmic sperm injection pose an increased risk of genitourinary congenital malformations in offspring compared to in vitro fertilization? A systematic review and meta-analysis. J Urol. 2015; 193 (5 Suppl): 1837-42.
Farhangniya M, Dortaj Rabori E, MozafariKermani R, Haghdoost AA, Bahrampour A, Bagheri P, et al. Comparison of Congenital Abnormalities of Infants Conceived by Assisted Reproductive Techniques versus Infants with Natural Conception in Tehran. Int J Fertil Steril. 2013; 7(3): 217-2.
Beukers F, van der Heide M, Middelburg KJ, Cobben JM, Mastenbroek S, Breur R, et al. Morphologic abnormalities in 2-year-old children born after in vitro fertilization/intracytoplasmic sperm injection with preimplantation genetic screening: follow-up of a randomized controlled trial. PGS Study Group. Fertil Steril. 2013; 99(2): 408-13.
Mozafari Kermani R, Nedaeifard L, Nateghi MR, Shahzadeh Fazeli A, Ahmadi E, Osia MA, et al. Congenital anomalies in infants conceived by assisted reproductive techniques. Arch Iran Med. 2012 Apr; 15(4): 2 28-31.
Fauser BC, Devroey P, Diedrich K, Balaban B, Bonduelle M, Delemarre-van HA, et al. Health outcomes of children born after IVF/ICSI: a review of current expert opinion and literature. Evian Annual Reproduction (EVAR) Workshop Group 2011. Reprod Biomed Online. 2014; 28(2): 162-82.
Flori F. Follow-up of the children born by ICSI. Med Sci. 2011; 27(8-9): 701-2.
Zollner U, Dietl J. Perinatal risks after IVF and ICSI. J Perinat Med. 2013; 41(1): 17-22.
van Heesch MM, Evers JL, Dumoulin JC, van der Hoeven MA, van Beijsterveldt CE, Bonsel GJ, et al. A comparison of perinatal outcomes in singletons and multiples born after in vitro fertilization or intracytoplasmic sperm injection stratified for neonatal risk criteria. Acta Obstet Gynecol Scand. 2014; 93(3): 277-86.
Westergaard HB1, Johansen AM, Erb K, Andersen AN. Danish National In Vitro Fertilization Registry 1994 and 1995: a controlled study of births, malformations and cytogenetic findings. Hum Reprod. 1999; 14: 1896-902.
Bonduelle M1, Legein J, Buysse A, Van Assche E, Wisanto A, Devroey P, et al. Prospective follow-up study of 423 children born after intracytoplasmic sperm injection. Hum Reprod. 1996; 11: 1558-64.
Wennerholm UB1, Bergh C, Hamberger L, Lundin K, Nilsson L, Wikland M, et al. Incidence of congenital malformations in children born after ICSI. Hum Reprod. 2000; 15:944-8.
Loft A1, Petersen K, Erb K, Mikkelsen AL, Grinsted J, Hald F, et al. A Danish national cohort of 730 infants born after intracytoplasmic sperm injection 1994-1997. Hum Reprod. 1999; 14: 2143-48.
Kim JW, Lee WS, Yoon TK, Seok HH, Cho JH, Kim YS, et al. Chromosomal abnormalities in spontaneous abortion after assisted reproductive treatment. BMC Med Genet. 2010 Nov 3; 11: 153.
Kayed HF, Mansour RT, Aboulghar MA, Serour GI, Amer AE, Abdrazik A. Screening for chromosomal abnormalities in 2650 infertile couples undergoing ICSI. Reprod Biomed Online. 2006; 12(3): 359-70.
Jozwiak EA, Ulug U, Mesut A, Erden HF, Bahçeci M. Prenatal karyotypes of fetuses conceived by intracytoplasmic sperm injection. FertilSteril. 2004 Sep; 82(3): 628-33.
Basaran S, Engur A, Aytan M, Karaman B, Ghanbari A, Toksoy G, et al. The results of cytogenetic analysis with regard to intracytoplasmic sperm injection in males, females and fetuses. Fetal Diagn Ther. 2004; 19(4): 313-8.
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