Study on the Survival and Reproductive Performance of Sterile Male <i>Glossina pallidipes</i>

Tadese Setegn; Tesfu Fekensa; Emiru Siyoum; Solomon Mekonnen

doi:doi:10.11648/j.ijbse.20160403.11

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Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes

Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen

Published in International Journal of Biomedical Science and Engineering (Volume 4, Issue 3)

Received: 6 May 2016 Accepted: 20 May 2016 Published: 30 June 2016

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Abstract

Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments. The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.

Published in	International Journal of Biomedical Science and Engineering (Volume 4, Issue 3)
DOI	10.11648/j.ijbse.20160403.11
Page(s)	13-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Tsetse Fly, Trypanosomosis, SIT, Irradiation and Kality

References

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[2]	Abebe, G., Malone, J. B. and Thompson, A (2004): Geospatial forecast model for tsetse transmitted animal trypanosomosis in Ethiopia. SINET, Ethio. J. Sci.27 (1): 1-8.
[3]	Coleman, P. G. and alphey, L. (2004). Editorial: Genetic control of vector populations:and imminent prospect. Tropical medicine and Intrnationl Health, 9:433- 437.
[4]	Curtis, C. (1985). Genetic control of insect pests: growth industry or lead balloon? Biological Journal of the Linnean society, 26:359-374.
[5]	Feldman, U., Luger, D., Barnot, H., Dengwal, L., Ajagbonna, B., Vreysen, M. J. B., and Van der vloedt, A., (1992). Tsetse fly mass rearing colony management deployment of sterile flies, related research development. In: tsetse control, diagnosis and chemotherapy using nuclear techniques, pp167-180 IAEA, Vienna.
[6]	Feldman, U. (1994). Guidelines for the Rearing of tsetse Flies Using the Membrane Feeding Technique. In: Techniques of insect rearing for the development of integrated pest and vector; management strategy pp 449-471. J. ochieng- odero, ICIPE Science Press, Nairobi.
[7]	Gomez, K. A. and Gomez, A. A. (1984). Statistical Procedures for Agricultural Research. 2^nd edn, Chichester, UK, Wiley.
[8]	Hasan, M. and Khan, A. R. (1998). Control of stored-product pests by irradiation. Integrated pest Management Reviews, 3: 15-29.
[9]	Kumela Lelisa, Delesa Damena, Mohamed Kedir and Teka Feyera (2015). Prevalence of Bovine Trypanosomosis and Apparent Density of Tsetse and Other Biting Flies in Mandura District, Northwest Ethiopia. J Veterinar Sci Technol. 6 (3):1-4
[10]	Leak, S. (1998). Tsetse biology and ecology: their role in the epidemiology and control of trypanosomosis. CABI Publishing. Oxford and New York. PP. 568.
[11]	Masangi A. and Lehane, M. (1999). A method for method for determining the age of very young tsetse flies (Diptera: Glossinidae) and an investigation of the factors determining head fluorescent levels in newly emerged adults. Bull. Entomol. res. 81, 185-188.
[12]	Nagel, P. (1995). Environmental monitoring handbook for tsetse control operations. The scientific Environmental monitoring group (SEMG), MargrafVerlarge.
[13]	Oluwafemi (2014). Tsetse/trypanosomosis challenges and rural poverty in Africa: Implications for food security and MDG. Res. J. Agr. Environ. Manag. 3(3): 158-161.
[14]	Parker, A and Mehta, K (2007). Sterile insect technique: a model for doseoptimization for improved sterile insect quality. Florida Entomologist, 90: 88-95.
[15]	Takken, W., Oladumade, M., Dengwat, L., Feldman, H., Onah, J., Tenade, S. and Hammann, H. (1986) the eradication of Glossina palpalis palpalisusing traps, insecticides-impregnated target and the sterile insect technique in centeral Nigeria. Bull. Entomol Res.76, 275 286.
[16]	Van den Bossche P, Doran M and Connor R.(2000). An analysis of trypanocidal drug use in the Eastern Province of Zambia. Acta Trop, 75(2):247-58.
[17]	Vreysen M., Saleh K., Ali M., Abdulla A., Zhu Z., Juma K. G, Dyck V., Msangi A. R, Mkonyi P., Feldmann H.(2000). Glossina austeni (Diptera: Glossinidae) eradicated on the island of Unguja, Zanzibar, using the sterile insect technique. J. anim. Ecol.93, 123-135.

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Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen. (2016). Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. International Journal of Biomedical Science and Engineering, 4(3), 13-21. https://doi.org/10.11648/j.ijbse.20160403.11

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ACS Style

Tadese Setegn; Tesfu Fekensa; Emiru Siyoum; Solomon Mekonnen. Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. Int. J. Biomed. Sci. Eng. 2016, 4(3), 13-21. doi: 10.11648/j.ijbse.20160403.11

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AMA Style

Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen. Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. Int J Biomed Sci Eng. 2016;4(3):13-21. doi: 10.11648/j.ijbse.20160403.11

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@article{10.11648/j.ijbse.20160403.11,
  author = {Tadese Setegn and Tesfu Fekensa and Emiru Siyoum and Solomon Mekonnen},
  title = {Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {4},
  number = {3},
  pages = {13-21},
  doi = {10.11648/j.ijbse.20160403.11},
  url = {https://doi.org/10.11648/j.ijbse.20160403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20160403.11},
  abstract = {Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments.  The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.},
 year = {2016}
}

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TY - JOUR
T1 - Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes
AU - Tadese Setegn
AU - Tesfu Fekensa
AU - Emiru Siyoum
AU - Solomon Mekonnen
Y1 - 2016/06/30
PY - 2016
N1 - https://doi.org/10.11648/j.ijbse.20160403.11
DO - 10.11648/j.ijbse.20160403.11
T2 - International Journal of Biomedical Science and Engineering
JF - International Journal of Biomedical Science and Engineering
JO - International Journal of Biomedical Science and Engineering
SP - 13
EP - 21
PB - Science Publishing Group
SN - 2376-7235
UR - https://doi.org/10.11648/j.ijbse.20160403.11
AB - Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments. The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.
VL - 4
IS - 3
ER -

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Author Information

Tadese Setegn

Ethiopian Veterinary Drug and Animal feed Administration and Control Authority, Addis Ababa, Ethiopia
Tesfu Fekensa

Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia
Emiru Siyoum

Addis Ababa University, College of Natural Sciences, Addis Ababa, Ethiopia
Solomon Mekonnen

Kality Tsetse Fly Rearing and Irradiation Center, Addis Ababa, Ethiopia

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APA Style

Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen. (2016). Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. International Journal of Biomedical Science and Engineering, 4(3), 13-21. https://doi.org/10.11648/j.ijbse.20160403.11

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ACS Style

Tadese Setegn; Tesfu Fekensa; Emiru Siyoum; Solomon Mekonnen. Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. Int. J. Biomed. Sci. Eng. 2016, 4(3), 13-21. doi: 10.11648/j.ijbse.20160403.11

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AMA Style

Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen. Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes. Int J Biomed Sci Eng. 2016;4(3):13-21. doi: 10.11648/j.ijbse.20160403.11

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@article{10.11648/j.ijbse.20160403.11,
  author = {Tadese Setegn and Tesfu Fekensa and Emiru Siyoum and Solomon Mekonnen},
  title = {Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {4},
  number = {3},
  pages = {13-21},
  doi = {10.11648/j.ijbse.20160403.11},
  url = {https://doi.org/10.11648/j.ijbse.20160403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20160403.11},
  abstract = {Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments.  The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.},
 year = {2016}
}

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