Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Co-Incubation with the Biofield Energy Treated Formulation: Impact of the Trivedi Effect®
International Journal of Biomedical Science and Engineering
Volume 4, Issue 5, October 2016, Pages: 40-49
Received: Nov. 29, 2016; Accepted: Dec. 8, 2016; Published: Dec. 29, 2016
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, USA
Alice Branton, Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, USA
Michael Peter Ellis, Trivedi Global, Inc., Henderson, USA
James Jeffery Peoples, Trivedi Global, Inc., Henderson, USA
James Joseph Meuer, Trivedi Global, Inc., Henderson, USA
Johanne Dodon, Trivedi Global, Inc., Henderson, USA
John Lawrence Griffin, Trivedi Global, Inc., Henderson, USA
John Suzuki, Trivedi Global, Inc., Henderson, USA
Joseph Michael Foty, Trivedi Global, Inc., Henderson, USA
Judy Weber, Trivedi Global, Inc., Henderson, USA
Julia Grace McCammon, Trivedi Global, Inc., Henderson, USA
Karen Brynes Allen, Trivedi Global, Inc., Henderson, USA
Kathryn Regina Sweas, Trivedi Global, Inc., Henderson, USA
Lezley Jo-Anne Wright, Trivedi Global, Inc., Henderson, USA
Lisa A. Knoll, Trivedi Global, Inc., Henderson, USA
Madeline E. Michaels, Trivedi Global, Inc., Henderson, USA
Margaret Kweya Wahl, Trivedi Global, Inc., Henderson, USA
Mark E. Stutheit, Trivedi Global, Inc., Henderson, USA
Michelle Barnard, Trivedi Global, Inc., Henderson, USA
Muriel Mae Ranger, Trivedi Global, Inc., Henderson, USA
Paromvong Sinbandhit, Trivedi Global, Inc., Henderson, USA
V. J. Kris Elig, Trivedi Global, Inc., Henderson, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral test formulation using mice splenocytes. The test formulation was divided into two parts. One part was the control without the Biofield Treatment. The other part was labelled the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The splenocyte cells were exposed with the test formulation at ranges of 0.00001053 to 10.53 µg/mL for cell viability by MTT assay, with cell viability ranging from 77.50% to 176.52%. TNF-α was significantly inhibited by 15.88%, 15.28%, 12.30%, 12.60%, and 22.72% at 0.00001053, 0.001053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the vehicle control (VC). TNF-α was significantly reduced by 2.33% and 8.35% at 1.053 and 10.53 µg/mL, respectively compared to the untreated test formulation. IL-1β was significantly reduced by 30.81%, 27.36%, 23.92%, 18.40%, 11.27%, and 21.16% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. IL-1β was significantly reduced by 48.63% (p≤0.001) and 15.28% at 0.00001053 and 0.0001053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. MIP-1α expression was inhibited by the Biofield Treated test formulation and showed immunosuppressive activity at 0.01053, 0.1053, 1.053, and 10.53 µg/mL by 22.33%, 16.25%, 15.58%, and 21.83%, respectively compared to the VC. The Biofield Treated test formulation significantly reduced the MIP-1α expression by 13.27% and 15.67% (p<0.05) at 0.01053 and 10.53 µg/mL, respectively compared to the untreated test formulation. The results showed the expression of IFN-γ was significantly reduced by 33.45%, 25.38%, 37.15%, 27.74%, 32.44%, 23.03%, and 44.21% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. Further, the IFN-γ level was significantly decreased by 19.02% at 10.53 µg/mL in the Biofield Treated test formulation compared to the untreated test formulation. Overall, the results demonstrate that The Trivedi Effect® Biofield Energy Healing (TEBEH) significantly enhanced the anti-inflammatory and immunomodulatory properties of the treated formulation, and may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Keywords
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Inflammation, Immunomodulation, Splenocytes, MIP-1α, IL-1β, TNF-α, IFN-γ
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Sambhu Charan Mondal, Snehasis Jana, Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Co-Incubation with the Biofield Energy Treated Formulation: Impact of the Trivedi Effect®, International Journal of Biomedical Science and Engineering. Vol. 4, No. 5, 2016, pp. 40-49. doi: 10.11648/j.ijbse.20160405.11
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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