| Peer-Reviewed

Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats

Received: 30 October 2017    Accepted: 10 November 2017    Published: 5 December 2017
Views:       Downloads:
Abstract

A new proprietary herbomineral formulation was formulated, consisting of herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The objective of this study was to evaluate the immunomodulatory effect of the Biofield Energy Healing (The Trivedi Effect®) Treatment on the test herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. Besides, one group of animals was also received Biofield Energy Treatment by same Biofield Energy Healers under similar conditions. The effect of the test formulation was monitored by an estimation of humoral immune response, delay type hypersensitivity, hematology, biochemistry, body weight, feed intake, relative organ weight, and histopathology in male Sprague Dawley rats. The primary antibody titre level was significantly increased by 36.36% in the Biofield Energy Treated formulation (G3); while decreased by 15.09% in the untreated test formulation (G4) compared to the disease control group (G2). The paw volume was significantly increased by 75% in the Biofield Energy Treated group per se at day -15 (G6) compared to the disease control. The level of red blood corpuscle (RBC) was significantly increased by 14.45% in the G6 group compared to the G2 group. The platelet count was significantly increased by 10.32% in the G3 group; while it was decreased by 5.71% in the G4 group compared with the G2 group. There was a significant elevation of serum phosphorus by 6.04% in the G3 group compared to the G2 group. The concentration of magnesium in serum was increased by 13.00% in the Biofield Energy Treated formulation (G3) compared to the disease control group (G2). The concentration of uric acid was significantly decreased by 8.05% in the Biofield Energy Treated formulation (G3) compared to the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulation was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with stress prevention and management and anti-aging by improving overall health.

Published in International Journal of Biomedical Science and Engineering (Volume 5, Issue 5)
DOI 10.11648/j.ijbse.20170505.12
Page(s) 53-62
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Immunomodulation, Humoral Immune Response, Histopathology, Stress Management, Anti-aging

References
[1] Rishton GM (2008) Natural products as a robust source of new drugs and drug leads: Past successes and present day issues. Am J Cardiol 101: 43D-9D.
[2] Mukhtar M, Arshad M, Ahmad M, Pomerantz R, Wigdahl B, Parveen Z (2008) Antiviral potentials of medicinal plants. Virus Res 131: 111-120.
[3] Girdhari L, Rana A (2007) Withania somnifera (ashwagandha): A review. Pharmacogn Rev 1: 129-136.
[4] Owais M, Sharad KS, Shehbaz A, Saleemuddin M (2005) Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 12: 229-235.
[5] Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[6] Singh N, Bhalla M, de Jager P, Gilca M (2011) An overview on ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[7] Lukác N, Massányi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
[8] Huang CF, Lin SS, Liao PH, Young SC, Yang CC (2008) The immunopharmaceutical effects and mechanisms of herb medicine. Cell Mol Immunol 5: 23-31.
[9] Galland L (1988) Magnesium and immune function: An overview. Magnesium 7: 290‐299.
[10] Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301‐323.
[11] Mazumder PM, Pattnayak S, Parvani H, Sasmal D, Rathinavelusamy P (2012) Evaluation of immunomodulatory activity of Glycyrhiza glabra L roots in combination with zing. Asian Pac J Trop Biomed 2: S15-S20.
[12] Mager J, Moore D, Bendl D, Wong B, Rachlin K, Yount G (2007) Evaluating biofield treatments in a cell culture model of oxidative stress. Explore (NY) 3: 386-390.
[13] Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
[14] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[15] Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
[16] Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
[17] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield energy treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[18] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
[19] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Evaluation of phenotyping and genotyping characterization of Serratia marcescens after biofield treatment. J Mol Genet Med 9: 179.
[20] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[21] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro Evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[22] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro Evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[23] Trivedi MK, Patil S, Harish S, Gangwar M, Jana S (2015) Biofield Treatment: An alternative approach to combat multidrug-resistant susceptibility pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193.
[24] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
[25] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An evaluation of biofield treatment on susceptibility pattern of multidrug resistant Stenotrophomonas maltophilia: An emerging global opportunistic pathogen. Clin Microbiol 4: 211.
[26] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 202-205.
[27] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical and structural properties of aluminium carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[28] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
[29] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S Evaluation of physical and structural properties of biofield energy treated barium calcium tungsten oxide. Advances in Materials 4: 95-100.
[30] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
[31] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Impact of biofield energy treatment on soil fertility. Earth sciences 4: 275-279.
[32] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[33] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture Forestry and Fisheries 4: 269-274.
[34] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (Brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries 4: 291-295.
[35] Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[36] Joharapurkar AA, Zambad SP, Wanjari MM, Umathe SN (2003) In vivo evaluation of antioxidant activity of alcoholic extract of Rubia cordifolia Linn. and its influence on ethanol-induced immunosuppression. Indian J Pharmacol 35: 232-236.
[37] Feldman BF, Zinkl JG, Jain VC (2000) Laboratory techniques for avian hematology, in Schalm’s Veterinary Hematology. (5th Edn) Lippincott Williams & Wilkins, Toronto, Canada.
[38] Chanda S, Dave R, Kaneria M, Shukla V (2012) Acute oral toxicity of Polyalthia longifolia var. pendula leaf extract in wistar albino rats. Pharmaceutical Biol 50: 1408-1415.
[39] OECD, OECD Guideline for Testing of Chemicals, vol. 420, Organization for Economic Cooperation and Development, Paris, France, 1992.
[40] Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[41] Agarwal R, Diwanay S, Patki P, Patwardhan B (1999) Studies on immunomodulatory activity of Withania somnifera (ashwagandha) extracts in experimental immune inflammation. J Ethnopharmacol 67: 27-35.
[42] Owais M, Sharad KS, Shehbaz A, Saleemuddin M (2005) Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 12: 229-235.
[43] Liu L, Li N, Lei T, Li K, Zhang Y (2014) The in vitro biological properties of Mg-Zn-Sr alloy and superiority for preparation of biodegradable intestinal anastomosis rings. Med Sci Monit 20: 1056-1066.
[44] Amresh GR, Singh PN, Rao CV (2008) Toxicological screening of traditional medicine Laghupatha (Cissampelos pareira) in experimental animals. J Ethnopharmacol 116: 454-460.
[45] Gronowicz G, Secor ER, Flynn JR, Jellison ER, Kuhn LT (2015) Therapeutic touch has significant effects on mouse breast cancer metastasis and immune responses but not primary tumor size. Evid Based Complement Alternat Med 2015: 926565.
[46] Garland SN, Valentine D, Desai K, Langer C, Evans T, Mao JJ (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
[47] Giasson M, Bouchard L (1998) Effect of therapeutic touch on the well-being of persons with terminal cancer. J Holist Nurs 16: 383-398.
[48] Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL (2015) Trends in the use of complementary health approaches among adults: Unites States, 2002-2012. National health statistics reports; no 79. Hyattsville, MD: National Center for Health Statistics.
[49] Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC (1998) Trends in alternative medicine use in the United States, 1990-1997: Results of a follow-up national survey. JAMA 280: 1569-1575.
[50] Barnes PM, Bloom B, Nahin RL (2008) Complementary and alternative medicine use among adults and children: United States, 2007. Natl Health Stat Report 12: 1-23.
Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, et al. (2017). Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats. International Journal of Biomedical Science and Engineering, 5(5), 53-62. https://doi.org/10.11648/j.ijbse.20170505.12

    Copy | Download

    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Cathryn Dawn Nykvist, et al. Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats. Int. J. Biomed. Sci. Eng. 2017, 5(5), 53-62. doi: 10.11648/j.ijbse.20170505.12

    Copy | Download

    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, et al. Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats. Int J Biomed Sci Eng. 2017;5(5):53-62. doi: 10.11648/j.ijbse.20170505.12

    Copy | Download

  • @article{10.11648/j.ijbse.20170505.12,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Cathryn Dawn Nykvist and Celine Lavelle and Daniel Paul Przybylski and Dianne Heather Vincent and Dorothy Felger and Douglas Jay Konersman and Elizabeth Ann Feeney and Jay Anthony Prague and Joanne Lydia Starodub and Karan Rasdan and Karen Mie Strassman and Leonid Soboleff and Maire Mayne and Mary M. Keesee and Padmanabha Narayana Pillai and Pamela Clarkson Ansley and Ronald David Schmitz and Sharyn Marie Sodomora and Sambhu Charan Mondal and Snehasis Jana},
      title = {Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats},
      journal = {International Journal of Biomedical Science and Engineering},
      volume = {5},
      number = {5},
      pages = {53-62},
      doi = {10.11648/j.ijbse.20170505.12},
      url = {https://doi.org/10.11648/j.ijbse.20170505.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170505.12},
      abstract = {A new proprietary herbomineral formulation was formulated, consisting of herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The objective of this study was to evaluate the immunomodulatory effect of the Biofield Energy Healing (The Trivedi Effect®) Treatment on the test herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. Besides, one group of animals was also received Biofield Energy Treatment by same Biofield Energy Healers under similar conditions. The effect of the test formulation was monitored by an estimation of humoral immune response, delay type hypersensitivity, hematology, biochemistry, body weight, feed intake, relative organ weight, and histopathology in male Sprague Dawley rats. The primary antibody titre level was significantly increased by 36.36% in the Biofield Energy Treated formulation (G3); while decreased by 15.09% in the untreated test formulation (G4) compared to the disease control group (G2). The paw volume was significantly increased by 75% in the Biofield Energy Treated group per se at day -15 (G6) compared to the disease control. The level of red blood corpuscle (RBC) was significantly increased by 14.45% in the G6 group compared to the G2 group. The platelet count was significantly increased by 10.32% in the G3 group; while it was decreased by 5.71% in the G4 group compared with the G2 group. There was a significant elevation of serum phosphorus by 6.04% in the G3 group compared to the G2 group. The concentration of magnesium in serum was increased by 13.00% in the Biofield Energy Treated formulation (G3) compared to the disease control group (G2). The concentration of uric acid was significantly decreased by 8.05% in the Biofield Energy Treated formulation (G3) compared to the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulation was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with stress prevention and management and anti-aging by improving overall health.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Potential Role of the Trivedi Effect® - Biofield Energy Healing on Immunomodulatory Response of Herbomineral Formulation in Male Sprague Dawley Rats
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Cathryn Dawn Nykvist
    AU  - Celine Lavelle
    AU  - Daniel Paul Przybylski
    AU  - Dianne Heather Vincent
    AU  - Dorothy Felger
    AU  - Douglas Jay Konersman
    AU  - Elizabeth Ann Feeney
    AU  - Jay Anthony Prague
    AU  - Joanne Lydia Starodub
    AU  - Karan Rasdan
    AU  - Karen Mie Strassman
    AU  - Leonid Soboleff
    AU  - Maire Mayne
    AU  - Mary M. Keesee
    AU  - Padmanabha Narayana Pillai
    AU  - Pamela Clarkson Ansley
    AU  - Ronald David Schmitz
    AU  - Sharyn Marie Sodomora
    AU  - Sambhu Charan Mondal
    AU  - Snehasis Jana
    Y1  - 2017/12/05
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijbse.20170505.12
    DO  - 10.11648/j.ijbse.20170505.12
    T2  - International Journal of Biomedical Science and Engineering
    JF  - International Journal of Biomedical Science and Engineering
    JO  - International Journal of Biomedical Science and Engineering
    SP  - 53
    EP  - 62
    PB  - Science Publishing Group
    SN  - 2376-7235
    UR  - https://doi.org/10.11648/j.ijbse.20170505.12
    AB  - A new proprietary herbomineral formulation was formulated, consisting of herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The objective of this study was to evaluate the immunomodulatory effect of the Biofield Energy Healing (The Trivedi Effect®) Treatment on the test herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. Besides, one group of animals was also received Biofield Energy Treatment by same Biofield Energy Healers under similar conditions. The effect of the test formulation was monitored by an estimation of humoral immune response, delay type hypersensitivity, hematology, biochemistry, body weight, feed intake, relative organ weight, and histopathology in male Sprague Dawley rats. The primary antibody titre level was significantly increased by 36.36% in the Biofield Energy Treated formulation (G3); while decreased by 15.09% in the untreated test formulation (G4) compared to the disease control group (G2). The paw volume was significantly increased by 75% in the Biofield Energy Treated group per se at day -15 (G6) compared to the disease control. The level of red blood corpuscle (RBC) was significantly increased by 14.45% in the G6 group compared to the G2 group. The platelet count was significantly increased by 10.32% in the G3 group; while it was decreased by 5.71% in the G4 group compared with the G2 group. There was a significant elevation of serum phosphorus by 6.04% in the G3 group compared to the G2 group. The concentration of magnesium in serum was increased by 13.00% in the Biofield Energy Treated formulation (G3) compared to the disease control group (G2). The concentration of uric acid was significantly decreased by 8.05% in the Biofield Energy Treated formulation (G3) compared to the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulation was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with stress prevention and management and anti-aging by improving overall health.
    VL  - 5
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Sections