The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge
International Journal of Environmental Protection and Policy
Volume 6, Issue 2, March 2018, Pages: 42-49
Received: Jun. 20, 2018; Published: Jun. 21, 2018
Views 770      Downloads 31
Authors
Cao Lu, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Xue Fei, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Liu Eryan, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Li Dengxing, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Asif Hussain, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Taimore Hussain, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Article Tools
Follow on us
Abstract
The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.
Keywords
Alkaline Protease, Sodium Hexametaphosphate, Methyl Orange, Three-Dimensional Fluorescence Spectroscopy
To cite this article
Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, Taimore Hussain, The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge, International Journal of Environmental Protection and Policy. Vol. 6, No. 2, 2018, pp. 42-49. doi: 10.11648/j.ijepp.20180602.14
References
[1]
J. Jiang, C. Liu, Control aniline pollutants from printing and dyeing industries, China Environ News. 17 (2015):55–56.
[2]
Li Xiaoge. Ecological management of Textile dyeing sludge [D], Donghua University, 2009.
[3]
Chen Hong. Effects of Combined Fenton on Textile Dyeing Sludge Physicochemical Properties and Dewaterability [D]. Guangdong University of Technology, 2014.
[4]
Xie Dawei. Treatment of Dyeing Wastewater from Dacron Yarn [J]. Environmental Protection, 1999 (04):28-30.
[5]
Guo Qingwei, Yang Renbin, Wu Genyi, ect. Practice on the reformed treatment process for dyeing wastewater of nylon textile [J]. Industrial Water Treatment, 2004 (05):70-73.
[6]
Dos Santos A B, Cervantes F J, Van Lier J B. Review of paper on current technologies for decolourisation of textile wastewaters; Perspectives for anaerobic biotechnology [J]. Bioresourches Technology, 2007, 98 (12):2369-2385.
[7]
Ning Xunan, Wei Peitao, Liu jingyong, ect. Co-Combustion Characteristics and Kinetics of Textile Dyeing Sludge and Sawdust [J]. Journal of Combustion Science and Technology, 2012, 18 (02):131-138.
[8]
Zhang Hedong, Gao Zuopeng, Ao Wenya, ect. Characterzation and analysis of textile sludge char from moving bed pyrolyser under microwave irradiation [J]. CIESC Journal, 2017, 68 (06): 2510-2518.
[9]
Feng Yinfang, Ning Xunan, Wu Junji, ect. Effects of potassium ferrate conjunction with ultrasound on textile dyeing sludge dewaterability.t [J]. Chinese Journal of Environmental Engineering, 2016, 10 (07):3787-3792.
[10]
Chen Hong, Ning Xunan, Luo Haijian, ect. Influence of bioleaching-Fenton oxidation on textile dyeing sludge dewatering performances [J]. Chinese Journal of Environmental Engineering, 2014, 8 (04):1641-1646.
[11]
Wawrzynczyk J Recktenwald M, Norrlow O, et al. The function of cation binding agents in the enzymatic treament of municipal sludge [J]. Water Res, 2008, 42 (6-7):1555-1562.
[12]
Beijer R, Enzymatic treatment of wastewater sludge in presence of a cation binding agent improved solubilisation and increased methane production [D]. Sweden Linkopings University, 2008.
[13]
Ahuja S K, Ferreira G M, Moreira A R. Utilization of enzymens for environmental applications [J]. Crit Rev Biotechnol, 2004, 24 (2-3):125-154.
[14]
He Dian, Wang Feng. A review of the detection methods of activated sludge activity [J]. Science and Technology Information, 2010 (01):381-382.
[15]
Sun Xiaoying, Zhang Tiefan, Nie Yinjin, ect. Determination of Specific Oxygen Consumption Rate of Activated Sludge and Its Application in Wastewater Treatment Plants [J]. Tianjin Construction Science and Technology, 2009, 19 (06): 56-59.
[16]
Xu Xiaolu, Shen Xiuying. Evaluation of active indicator of activated sludge [J]. Chinese Journal of Environmental Science, 1993 (02):58-62+95.
[17]
Luo Huining. Determination of Chemical Oxygen Demand (COD) by Microwave Digestion [J]. Guangxi & Development of Chemical Industry, 2003 (03):25-26.
[18]
Huang Qing. Comparison of Microwave Digestion Method and Standard Return Method for Determination of COD [J]. Science and Technology Innovation Herald, 2013 (17):58.
[19]
Zhong Fangxiao, Ren Haihua, Li Yan. Comparison of methods in Determination of polysaccaride content [J]. Lishizhen Medicine and Materia Medica Research, 2007 (08):1916-1917.
[20]
Lu Ping, Yu Tongquan, Wang Shuying, ect. A review on determination of protein [J]. Journal of Beijing Agricultural College, 2006 (02):65-69.
[21]
Yu Jing. Enhanced enzymatic hyydrolysis of excess sludge by surfactant [D]. Hu Nan University, 2011.
[22]
Kong Wangshen, Liu Yan. Adsorption of a dye by sludge and the roles of extracellular polymeric substances [J]. Environmental Science, 2007 (12):2716-2721.
[23]
Cao Jiashun, Zhu Zheying, Fang Fang, ect. Characteristics of extracellular polymeric substances (EPS) in the azo dye wastewater treated by activated sludge [J]. Acta Scientiae Circumstantiae, 2013, 33 (09):2498-2503.
[24]
Xu Hongliang, Wu Xiaoying, Zhen Pin. Effect of extracellular polymer substances on wastewater treatment [J]. Environmental Science and Technology, 2005 (S1):121-122+124.
[25]
Shanableh A, Jomaa S. Production and transformation of volatile fatty acids from sludge subjected to hydrothermal treatment. Water Sci. Technol. 2001, 44:129-135.
ADDRESS
Science Publishing Group
548 FASHION AVENUE
NEW YORK, NY 10018
U.S.A.
Tel: (001)347-688-8931