Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria
International Journal of Environmental Protection and Policy
Volume 5, Issue 6-1, December 2017, Pages: 23-32
Received: Mar. 23, 2017; Accepted: Mar. 24, 2017; Published: Aug. 21, 2017
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Authors
Bassey Effiong Edet, Department of Applied Microbiology and Brewery, Nnamdi Azikwe University, Awka, Nigeria
Gwana Adamu Mohammed, Laboratory Unit, Department of Animal Health and Production Technology, Mohamet Lawan College of Agriculture, Maiduguri, North – Eastern Nigeria
Buhari Bagudu Yawuri, Department of Science Laboratory Technology, Waziri Umaru Federal Polytechnic, Birnin Kebbi, Nigeria
Alhaji Bashir Mohammed, Department of Basic Sciences and Technology, Mohamet Lawan College of Agriculture, Maiduguri, North, Eastern Nigeria
Abubakar Musa, Department of Basic Sciences and Technology, Mohamet Lawan College of Agriculture, Maiduguri, North, Eastern Nigeria
Abba Mala Galtimari, Department of General Studies, Mohamet Lawan College of Agriculture, Maiduguri, North – Eastern Nigeria
Hauwa Lawan Badawi, Heritage Unit, Department of Heritage and Monument Sites, National Commission for Museums and Monuments, Maiduguri, Nigeria
Mustapha Ahmadu, Department of Remedial Sciences, Mohamet Lawan College of Agriculture, Maiduguri, North – Eastern Nigeria
Fanna Ali Garra, Laboratory Unit, Department of Animal Health and Production Technology, Mohamet Lawan College of Agriculture, Maiduguri, North – Eastern Nigeria
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Abstract
This study focuses on the estimation and characterization of microorganisms isolated from wastewater and sludge in Awka metropolis, Nigeria. The samples were collected randomly from four (A, B, C and D) respective areas, analyzed microbiologically by homogenizing the samples under aseptic conditions to obtain a homogenous mixture, the isolation techniques used was pour plate methods as described by Cheesbrough, (2010); Joanne et al, (2011); APHA, (1989). The samples were diluted serially, inoculated on to MacConkey agar and Sabouraud Dextrose agar media each and microorganisms were isolated; wastewater had a range from 44 to 100 colonies and total of 284 (71 ± 24.30), CFU / ml (10-4) of 1.0 × 107 to 8.0 × 106 with total count of 19.4 (4.85 ± 2.96) × 106, while sludge was ranges from 60 to 120 colonies with a total of 358 (89.5 ± 29), CFU / ml (10-4) ranged from 1.0 × 107 to 7.0 × 106 with total count of 15.2 (3.8 ± 3.1) × 106. The colony and the morphology of the isolates were recorded, the bacterial isolates were characterized by motility tests, Gram staining and identified by biochemical tests. The fungal isolates were microscopically examined using a wet mount procedure. From the results obtained 8 bacterial and 7 fungal isolates were identified from both the wastewater and the sludge; Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Micrococcus leteus, Enterococcus faecalis, Serratia mercescens and Yersinia enterocolitica. Acrophialophora fusispora, Epicoccum Purpurascens, Rhizopus microsporus, Exophiala spinifera, Cladosporium cladosporiodes, Aspergillus niger and Phaeoacremonium parasiticum. Results also showed that staphylococcus aureus have the highest percentage of occurrence in wastewater, while in sludge Micrococcus species have the highest percentage of occurrence.
Keywords
Biochemical Test, Culture Media, Domestic, Industrial, Microorganism Morphological, Sludge, Wastewater
To cite this article
Bassey Effiong Edet, Gwana Adamu Mohammed, Buhari Bagudu Yawuri, Alhaji Bashir Mohammed, Abubakar Musa, Abba Mala Galtimari, Hauwa Lawan Badawi, Mustapha Ahmadu, Fanna Ali Garra, Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria, International Journal of Environmental Protection and Policy. Special Issue:The Role of SLT: Environmental Impact Assessment And Statement Concept. Vol. 5, No. 6-1, 2017, pp. 23-32. doi: 10.11648/j.ijepp.s.2017050601.14
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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