Preventive Effect of Phloretin on Components of Glycoprotein Changes in Streptozotocin Induced Diabetic Rats
International Journal of Diabetes and Endocrinology
Volume 2, Issue 2, June 2017, Pages: 30-35
Received: Feb. 28, 2017;
Accepted: Apr. 28, 2017;
Published: Jul. 3, 2017
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Tamilarasan Nithiya, Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu, India
Rajangam Udayakumar, Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu, India
The present study was aimed to evaluate the protective effect of phloretin on glycoprotein components in serum and tissues of streptozotocin induced diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of STZ (60 mg/kg b.w). Phloretin (25mg and 50mg/kg b.w) was administered orally to diabetic rats for 45 days. The effect of phloretin on serum glucose, glycated hemoglobin, serum and tissue glycoprotein components were studied. Phloretin administration to diabetic rats decreased the level of glucose, glycated hemoglobin and glycoprotein components in serum. There was observed a significant decrease in the level of sialic acid and significantly elevated levels of hexose, hexosamine and fucose in liver and kidney of diabetic rats. The altered levels of serum and tissue glycoprotein components were restored to near normal in diabetic rats treated with phloretin at 50mg/kg b.w. The present findings suggest that phloretin can potentially ameliorates the hyperglycemia and changes in glycoprotein components abnormalities in streptozotocin induced diabetic rats. So, the phloretin may be used as an effective therapeutic agent for diabetes mellitus in future.
Preventive Effect of Phloretin on Components of Glycoprotein Changes in Streptozotocin Induced Diabetic Rats, International Journal of Diabetes and Endocrinology.
Vol. 2, No. 2,
2017, pp. 30-35.
Kardeşler L, Buduneli N, Bıyıkoglu B, Çetinkalp S, Kutukçuler N. (2008). “Gingival crevicular fluid PGE2, IL-1ß, t-PA, PAI-2 levels in type 2 diabetes and relationship with periodontal disease”.Clin. Biochem., vol. 41(10-11): pp. 863-868.
Kuma G, Banu S, Murugesan A.G. (2009). “Influence of Helicteresisora administration for diabetes mellitus: Its effect on erythrocyte membrane and antioxidant status”. Food ChemToxicol., vol. 47: pp.1803-9.
Schuster D.P., Duvuuri V. (2002). “Diabetes mellitus”.Clin Podiatr Med and Surg., vol.19: pp.79-107.
Prakasam A, Sethupathy S, Pugalendi K.V. (2005). “Influence of Casearia esculenta root extract on glycoprotein components in streptozotocin diabetic rats”.Pharmazie, vol. 60: pp. 229-232.
Pari L, Murugan P. (2007). “Changes in glycoprotein components in streptozotocin - nicotinamide induced type 2 diabetes: influence of tetrahydrocurcumin from Curcuma longa”. Plant Foods Hum Nutr., vol. 62: pp. 25-29.
Blazso G, Gabor M. (1995). “Effects of prostaglandin antagonist phloretin derivatives on mouse ear edema induced with different skin irritants”. Prostaglandins, vol. 50: pp. 161-168.
Oresajo C. (2008). “Protective effects of a topical antioxidant mixture containing vitamin C, ferulic acid and phloretin against ultraviolet induced photodamage in human skin”. J Cosmet Dermatol., vol. 7: pp. 290-297.
Chih-Hsiung Wu, Yuan-Soon Ho, Chia-Yi Tsai, Ying-Jan Wang, How Tseng, Po-Li Wei, Chia-Hwa Lee, Ren- Shyan Liu, Shyr-Yi Lin. (2009). “In vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter”. Int J Cancer, vol. 124: pp. 2210-2219.
Ali Reza EbadollahiNatanzia, ShimaMahmoudian, BagherMinaeie, OmidSabzevari. (2011). “Hepatoprotective activity of phloretin and hydroxychalcones against acetaminophen induced hepatotoxicity in mice”. Iran J Pharm Sci., 7(2): 89-97.
Trinder P. (1969). “Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor”. Ann Clin Biochem., vol. 6: pp. 24.
Nayak S.S., Pattabiraman T.N. (1981). “A new colorimetric method for the estimation of glycosylated haemoglobin”. Clin Chim Acta, 109:267- 274.
Folch J, Lees M, Solane S. G. H. (1957). “A simple method for isolation and purification of total lipids from animal tissues”. J Biol Chem., vol.26: pp.497-509.
Niebes P. (1972). “Determination of enzymes and degradation products of glycosaminoglycan metabolism in the serum of healthy and varicose subjects”. Clin Chim Acta, vol. 42: pp. 399-408.
Wagner W. D. (1979). “A more sensitive assay discriminating galactosamine and glucosamine in mixtures”. Analytical Biochemistry, vol. 94: pp. 394-396.
Warren L. (1959). “The thiobarbituric acid assay of sialic acids”. J Biol Chem., vol. 234: pp. 1971-1975.
Dische Z, Shettles L. B. (1948). “A specific color reaction of ethylpentoses and a spectrophotometric micromethod for their determination”. J Biol Chem., vol. 175: pp. 595-603.
Szkudelski T. (2001). “The mechanism of alloxan and streptozotocin action in β cells of the rat pancreas”. Physiol Res., vol. 50: pp. 536-546.
Ambika S, Saravanan R, Thirumavalavan K. (2013). “Antidiabetic and antihyperlipidemic effect of p-hydroxycinnamic acid on streptozotocin induced diabetic Wistar rats”. Biomedicine and Aging Pathology, 3(4): 253-257.
Brownlee M. (2001). “Biochemistry and the molecular cell biology of diabetic complications”. Nature, vol. 414:pp. 813-820,
Pari L, Srinivasan S. (2010). “Preventive effect of diosmin, a bioflavonoid, on glycoprotein changes in streptozotocin nicotinamide induced type 2 diabetic rats”. In J Pharm Sci Res., vol.10: pp. 89-95.
Muthukumaran J, Srinivasan S, Venkatesan R. S., Ramachandran V, Muruganathan U. (2013).“Syringic acid, a novel natural phenolic acid, normalizes hyperglycemia with special reference to glycoprotein components in experimental diabetic rats”. J Acu Dis., pp. 304-309.
Ciftci G, Yarim G. F. (2011). “Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs”. J Vet Sci., vol. 12(4): pp. 325-331.
Buse M.G. (2006). “Hexosamines, insulin resistance, and the complications of diabetes: current status”. Am. J. Physiol Endocrinol Metab., vol. 290: E1-E8.
Saravanan G, Ponmurugan G. P., Senthil Kumar, Rajrajan T. (2010). “Antidiabetic effect of S-allylcysteine: Effect on plasma and tissue glycoproteins in experimental diabetes”. Phytomedicine, vol.17: pp. 1086-1089.
Obici J, Wang R, Chowdury Z, Feng U, Siddhanta K, Morgan. (2002). “Identification of a biochemical link between energy intake and energy expenditure”. J Clin Invest., vol.109: pp.1599-605.
Brownlee M. (2005). “The pathobiology of diabetic complications: A unifying mechanism”. Diabetes, vol. 54: pp. 1615-25.
Wellen K. E., Lu C, Mancuso A, Lemons J.M.,Ryczko M, Dennis J.W.,et al. (2010). “The hexosamine biosynthetic pathway couples growth factor-induced glutamine uptake to glucose metabolism”. Genes Dev., vol. 24: pp. 2784-2799.
NarendraSilawat,VipinBihari Gupta. (2013). “Antidiabetic effect of chebulic acid in streptozotocin induced diabetic rats”. Asian J Complem Alternat Med., 01 (01): 16-23.
Pari L, Karthikesan K. (2009). “Protective role of tetrahydrocurcumin and chlorogenic acid on glycoprotein changes in streptozotocin nicotinamide- induced diabetic rats”. J Pharm Sci Res., vol. 1(4): pp.173-180.
Muruganathan U, Srinivasan S, Indumathi D. (2013). “Antihyperglycemic effect of carvone: Effect on the levels of glycoprotein components in streptozotocin-induced diabetic rats”. J Acu Dis., pp.310-315.
Jayachandran M, Srinivasan S et al., (2013). “Syringic acid, a novel natural phenolic acid, normalizes hyperglycemia with special reference to glycoprotein components in experimental diabetic rats”. J Acu Dis, 304-309.
Calles-Escandon J, Cipolla M. (2001). “Diabetes and endothelial dysfunction: A clinical perspective”. Endocr Rev., 22: 6-52.
Leelavinothan Pari, Bashir Ahmad Sheikh. (2015). “Antihyperglycemic effect of trans-anethole in streptozotocin induced diabetic rats with special reference to glycoprotein components”. Int. J. Adv. Res. Biol.Sci, vol. 2(5): pp. 28–34.