Hypoglycemic and Antioxidant Capacity of Curcuma Longa and Viscum Album in Alloxan Induced Diabetic Male Wistar Rats
International Journal of Diabetes and Endocrinology
Volume 4, Issue 1, March 2019, Pages: 26-34
Received: Dec. 29, 2018; Accepted: Jan. 16, 2019; Published: May 6, 2019
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Nwozo Sarah Onyenibe, Nutritional and Industrial Biochemistry Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
Nyam Augustine Nathaniel, Nutritional and Industrial Biochemistry Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
Nwawuba Stanley Udogadi, Nutritional and Industrial Biochemistry Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
Olukotun Oluwakemi Iyanu, Nutritional and Industrial Biochemistry Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
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Plant based therapies may be a potent means of managing and preventing diabetes and currently, combination therapies are employed for the treatment of critical diseases. In view of this, the objectives of the present study was to investigate the hypoglycemic and antioxidant capacity C. longa, V. album and co-treatment with C. longa + V. album on diabetes and its related complication. Qualitative phytochemical analysis of C. longa and V. album were examined using standard procedures and the result revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, saponins, tannins, terpenoids and steroids respectively. Thirty six (36) male albino rats with the mean weight between 120-135g were divided into six (6) groups (n=6), group one (1) served as the normal control and there experimental groups were diabetic, induced with 150 mg/kg intraperitoneal alloxan injection. Body weight, blood sugar level, glycated hemoglobin, glucose-6-phosphate dehydrogenase, α-amylase, lipid profile, liver function and antioxidant markers were determined using standard procedures and the results revealed that, co-treatment with C. longa + V. album demonstrated an excellent weight reduction ability, hypoglycemic capacity, modulation of G6PDH, HBA1C and lipid profile, inhibition of α-amylase and enhancement of liver function and antioxidant levels. Therefore, co-treatment with C. longa + V. ablum can be a good therapeutic choice for the management of diabetes and its related conditions.
Diabetes Mellitus, Curcuma Longa, Viscum Album, Lipid Profile, Liver Function and Antioxidant
To cite this article
Nwozo Sarah Onyenibe, Nyam Augustine Nathaniel, Nwawuba Stanley Udogadi, Olukotun Oluwakemi Iyanu, Hypoglycemic and Antioxidant Capacity of Curcuma Longa and Viscum Album in Alloxan Induced Diabetic Male Wistar Rats, International Journal of Diabetes and Endocrinology. Vol. 4, No. 1, 2019, pp. 26-34. doi: 10.11648/j.ijde.20190401.15
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World Health Organization. Global Report on Diabetes. 2016, Isbn, 978, 88. https://doi.org/ISBN 978 92 4 156525 7.
Riaz S. Diabetes mellitus. Scientific Research and Essay. Vol. 4, No. 5, 2009, pp. 367-373.
International Diabetes Federation. Diabetes atlas. 8th ed. Brussels: International Diabetes Federation. 2017. Available at www. diabetesatlas.org.
International Diabetes Federation. IDF Diabetes, 7th ed. Brussels: International Diabetes Federation; 2015. Available at http://www.diabetesatlas.org.
Nwozo Sarah O, Adeneye Damilola A, Nwawuba Stanley U. Effect of Solanium melongena fruits supplemented diet on hyperglycemia, overweight, liver function and dyslipidemia in male New Zealand rabbits fed high fat and sucrose diet. Integrative Obesity and Diabetes.Vol. 4, No. 3, 2018, pp.1-5. doi: 10.15761/IOD.1000210.
Farsi L, Keshavarz M, Soltani N. Relaxatory effect of GABA mediated by same pathway in diabetic and normal rat vessel. Iran Basic Med Sci. Vol. 4, No. 1, 2015, pp.94–98.
Papatheodorou K, Papanas N, Banach M, Papazoglou D, & Edmonds M. (2016). Complications of Diabetes. Journal of Diabetes Research. Vol.1, No. 3, 2016, pp. http://dx.doi.org/10.1155/2016/6989453.
Kautzky-Willer A, Harreiter J, Pacini G. Sex and Gender Differences in Risk, Pathophysiology and Complications of Type 2 Diabetes Mellitus. Endocr Rev. Vol. 37, No. 3, 2016, pp. 278-316. doi: 10.1210/er.2015-1137.
Njamen D, Nkeh-chungag BN, Tsala E, Fomum ZT, & Mbanya JC. Effect of Bridelia ferruginea ( Euphorbiaceae ) Leaf Extract on Sucrose-induced Glucose Intolerance in Rats. Tropical Journal of Pharmaceutical Research. Vol. 11, No. 5, 2012, pp. 759-765. http://dx.doi.org/10.4314/tjpr.v11i5.9.
World Health Organization (Ed.), WHO Traditional Medicine Strategy2014–2023.WHO Press,Geneva,Switzerland. 2013.
Ezuruike UF, & Prieto JM. The use of plants in the traditional management of diabetes in Nigeria : Pharmacological and toxicological considerations. Journal of Ethnopharmacology. Vol. 155, No. 2, 2014, pp. 857–924. https://doi.org/10.1016/j.jep.2014.05.055.
Chikezie PC, Ojiako OA, Nwufo KC. Overview of Anti-Diabetic Medicinal Plants: The Nigerian Research Experience. Journal of Diabetes and Metabolism. Vol. 6, No. 6, 2015, pp. 1-7. doi: 10.4172/2155-6156.1000546.
Khaliq T, Sarfraz M, Ashraf MA. Recent Progress for the Utilization of Curcuma longa, Piper nigrum and Phoenix dactylifera Seeds against Type 2 Diabetes. West Indian Med J. Vol. 64, No. 5, 2015, pp.527. DOI: 10.7727/wimj.2016.176.
Afsheen N, Khalil-Ur-Rahman, JahanN. Attenuation of chemically induced diabetes in rabbits with herbal mixture (Citrullus colocynthis and Cicer arietinum). Pak Vet J. Vol.33, 2013, pp.41–4.
Wickenberg Jennie, Sandra Lindstedt I, Joanna Hlebowicz. Effects of Curcuma longa (turmeric) on postprandial plasma glucose and insulin in healthy subjects. Nutrition Journal. Vol. 9, No. 43, 2010, pp.1-5. doi:10.1186/1475-2891-9-43.
Gutierres VO, Pinheiro CM, Assis RP, Vendramini RC, & Pepato MT. “Curcumin-supplemented yoghurt improves physiological and biochemical markers of experimental diabetes,”The British Journal of Nutrition. Vol. 108, no. 3, 2012, pp. 440–448.
Xie XY, Kong PR, Wu JF, Li Y, and Li YX “Curcumin attenuates lipolysis stimulated by tumor necrosis factor-alpha or isoproterenol in 3T-L1 adipocytes.” Phytomedicine. Vol. 20, No. 1, 2012, pp. 3–8.
Kuhad A. and Chopra K, “Curcumin attenuates diabetic encephalopathy in rats: behavioral and biochemical evidences.” European Journal of Pharmacology. Vol. 576, No. 1–3, 2007, pp. 34–42.
Nwozo Sarah, Oluwatosin Adaramoye and Edith Ajaiyeoba. Oral Administration of Extract from Curcuma longa Lowers Blood Glucose and Attenuates Alloxan-Induced Hyperlipidemia in Diabetic Rabbits. Pakistan Journal of Nutrition. Vol. 8, 2009, pp. 625-628. http://dx.doi.org/10.3923/pjn.2009.625.628.
Kalekar Samidha A, Renuka Munshi P, and Urmila Thatte M. Do plants mediate their anti-diabetic effects through anti-oxidant and anti-apoptotic actions? an in vitro assay of 3 Indian medicinal plants .BMC Complementary and Alternative Medicine. Vol. 13, No. 25, 2013. http://www.biomedcentral.com/1472-6882/13/257.
Onay-Ucar Evren, Ozlem Erol, Bas¸ak Kandemir, and ElifMerto˘glu. Viscum album L. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage. Evidence-Based Complementary and Alternative Medicine. Vol. 2012, Article ID 958740, 2012, pp. 1-7. doi:10.1155/2012/958740.
Bar-Sela G. “White-Berry Mistletoe (Viscum album L.) as complementary treatment in cancer: does it help?” European Journal of IntegrativeMedicine. Vol. 3, no. 2, 2011, pp. e55–e62.
Maier G, and Fiebig HH. “Absence of tumor growth stimulation in a panel of 16 human tumor cell lines by mistletoe extracts in vitro,” Anti-Cancer Drugs. Vol. 13, no. 4, 2002, pp. 373–379.
Gardin NE. “Immunological response to mistletoe (Viscum album L.) in cancer patients: a four-case series,” Phytotherapy Research. Vol. 23, no. 3, 2009, pp. 407–411.
Che C, Wang ZJ, Chow MSS, Christopher WK. Herb-herb combination for therapeutic enhancement and Advancement: Theory, Practice and Future Perspectives. Molecules. Vol. 18, 2013, 5125-5141.
Olajubu FA. Enhancement of Therapeutic Success Through Herbal-Herbal Combination. Int.J.Curr.Microbiol.App.Sci. Vol. 3, No. 8, 2014, pp. 418-425.
Harbone JB. Phytochemical methods-A guide to modern techniques of plants analysis. Champman and Hall, London. 1998, pp. 182-190.
Allen GM, Nostro A, Germano MP, Marino A, and Cannatelli MA. Extraction Methods and Bioautography for evaluation of Medicinal plant. Phytochemical Analysis. Lett. Appl. Microbiol. Vol. 30, 1974, pp. 379-384.
Dawud FA, Eze ED, Ardja AA, Isa AS, and Jimoh A. Ameliorative Effects of Vitamin C and Zinc in Alloxan-induced Diabetes and Oxidative Stress in Wistar Rats. Curr. Res. J. Bio. Sci. Vol. 4, No. 2), 2012, 123-129.
Dhandapani S, Ramasamy SV, Rajagopal S, and Namasivayam N. 2002. Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol. Res. Vol. 46, No 3, 2002, 251-255.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of biuret reaction. J. Biol Chem. Vol. 177, 1949, pp. 751-766.
Misra HP, and Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. Vol. 247, 1972, pp. 3170-3175.
Sinha AK. Colrimetric assay of catalase. Anal Biochem. Vol. 47, 1972, 389-394.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J. Lab Clin Med. Vol. 61, 1963, pp. 882-888.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. Vol. 249, 1974, pp. 7130-7139.
Tundis R, Loizzo MR, Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem. Vol. 10, No. 4, 2010, pp. 315-31.
Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi SH, and Farhangi A. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry. Vol. 22, No, 2, 2007, pp. 60-64.
Mohal S, Mondal DK, Shamim KM. Impact of Momordica Charantia (Karela) on body weight in the streptozotocin-induced Diabetes Rats. Bangladesh Journal of Anatomy. Vol. 9, No. 2, 2011, pp. 106-109.
Ewenighi C, Dimkpa U, Onyeanusi J, Onoh L, Onoh G, and Ezeugwu U et al. Estimation of glucose level and body weight in alloxan induced diabetic rat treated with aqueous extract of Garcinia kola seed. Ulutas Med J. Vol. 1, No. 2, 2015, pp. 26-30. DOI: dx.doi.org/10.5455/umj.20150507042420.
Pournaghi Pouya, Rajab-Ali Sadrkhanlou, Shapour Hasanzadeh, and Azadeh Foroughi. An investigation on body weights, blood glucose levels and pituitary-gonadal axis hormones in diabetic and metformin-treated diabetic female rats. Vet Res Forum. Vol. 3, No. 2, 2012, pp. 79–84.
Lenzen S. The mechanisms of alloxan and streptozotocin induced diabetes. Diabetologica. Vol. 51, 2008, pp. 216-226.
Pan GY, Huang ZJ, Wang GJ, Fawcett JP, Liu XD, Zhao XC et al. The antihyperglycaemic activity of berberine arisesfrom a decrease of glucose absorption. Planta Med. Vol. 69, 2003, pp. 632–6.
Uanhong L, Caili F, Yukui R, Guanghui H, Tongyi C. Effects of protein bound polysaccharide isolated from pumpkin on insulin in diabetic rats. Plant Foods Human Nutr. Vol. 60, 2005, pp. 13–6.
Ng TB, Wong CM, Li WW and Yeung HW. Insulin-like molecules in Momordica charantia seeds. J Ethnopharmacol. Vol. 15, 1986, pp. 107–17.
Olatunde A, Joel EB, Tijjani H, Obidola SM, and Luka CD. Anti-diabetic Activity of Aqueous Extract of Curcuma longa (Linn) Rhizome in Normal and Alloxan-Induced Diabetic Rats. Researcher. Vol. 6, No. 7, 2014, pp. 58-65.
Adaramoye Oluwatosin, Massoud Amanlou, Mehran Habibi-Rezaei, Parvin Pasalar, and Ali Moosavi-Movahedi. Methanolic extract of African mistletoe (Viscum album) improves carbohydrate metabolism and hyperlipidemia in streptozotocin-induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 2012, pp. 427-433.
Park BH, and Park JW. “The protective effect of Amomum xanthoides extract against alloxan-induced diabetes through the suppression of NF𝜅B activation.” Experimental and Molecular Medicine. Vol. 33, No. 2, 2001, pp. 64–68.
Antonio Pinna, Luigia Contini, Ciriaco Carru, and Giuliana Solinas. Glucose-6-Phosphate Dehydrogenase Deficiency and Diabetes Mellitus with Severe Retinal Complications in a Sardinian Population, Italy. Int J Med Sci. Vol. 10, No. 13, 2013, pp. 1907–1913. doi: 10.7150/ijms.6776.
Miedema, K. Standardization of HbA1c and optimal range of monitoring. Scand. J. Clin. Lab. Invest. Vol. 240, 2005, pp. 61–72.
Elgawish A, Glomb M, Friendlander M, Monnier VM. Involvement of hydrogen peroxide in collagen crosslinking by high glucose in vitro and in vivo. J Biol Chem. Vol. 271, 199, pp. 12964–71.
Temelkova-Kurktschiev T, and Hanefeld, M. The lipid triad in type 2 diabetes - prevalence and relevance of hypertriglyceridaemia/low high-density lipoprotein syndrome in type 2 diabetes. Exp Clin Endocrinol Diabetes. Vol. 112, No. 2, 2004, pp. 75-9.
Nwawuba Stanley U and Okechukwu Francis C The effect of Cyperus esculentus (Tigernut) oil on liver, kidney and hematological biomarkers in low dose streptozocin and high fat diet exposed male wistar rats. International Journal of Food Science and Nutrition. Vol. 3, No. 4, 2018, pp. 148-152.
Lucchesi Amanda N, Lucas Langoni C, and César Tadeu S. Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans. Journal of Diabetes Research. Vol. 2015, 2015, Article ID 494578, pp. 11. http://dx.doi.org/10.1155/2015/494578.
Mobasher Ahmad, Sairah Hafeez K, and Afroze Mobasher. Protective effect of crude Curcuma longa and its methanolic extract in alloxanized rabbits. Pak. J. Pharm. Sci. Vol. 27, No.1, 2014, pp.121-128.
Onunogbo CC, Ohaeri OC, and Eleazu CO,. Effect of Mistletoe (Viscum album) Extract on the Blood Glucose, Liver Enzymes and Electrolyte Balance in Alloxan Induced Diabetic Rats. American Journal of Biochemistry and Molecular Biology. Vol. 3, 2013, pp. 143-150. DOI: 10.3923/ajbmb.2013.143.150.
Naziroglu M, and Cay M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biol Trace Elem Res. Vol. 79, 2001, pp. 149–59.
Bashan N, Kovsan J, Kachko I, Ovadia H, and Rudich A. 2009. Positive and negative regulation of insulin signaling by reactive oxygen and nitrogen species. Physiological Review. Vol. 89, 2009, pp. 27-71.
Ramachandran Vinayagam, and Baojun Xu. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutrition & Metabolism. Vol. 12, No. 60, 2015, pp. 1-20. DOI 10.1186/s12986-015-0057-7.
Li-qing Su ⇑, Yong-di Wang, Hai-yan Chi. Effect of curcumin on glucose and lipid metabolism, FFAs and TNF-a in serum of type 2 diabetes mellitus rat models. Saudi Journal of Biological Sciences. Vol. 24, 2017, pp. 1776–1780. https://doi.org/10.1016/j.sjbs.2017.11.011.
Govindappa M, Sadananda TS, Channabasava, Ramachandra YL, Chandrappa CP et al. In vitro and in vivo antidiabetic activity of lectin (N-acetyl-galactosamine, 64 kDa) isolated from endophytic fungi, Alternaria species from Viscum album on alloxan induced diabetic rats. Integr Diabetes Obesity. Vol. 1, No. 1, 2015, pp. 11-19. doi: 10.15761/IOD.1000104.
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