Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard

Muktari Isa Shago; Markus Kirwe Jiyil; Nazifi Alhaji Sani; Garba Suleiman

doi:doi:10.11648/j.ijidt.20200503.21

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Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard

Muktari Isa Shago, Markus Kirwe Jiyil, Nazifi Alhaji Sani, Garba Suleiman

Published in International Journal of Infectious Diseases and Therapy (Volume 5, Issue 3)

Received: 18 February 2020 Accepted: 28 February 2020 Published: 16 September 2020

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Abstract

Malaria remains a public health problem in over 90 countries worldwide, especially in Africa where it is the cause of morbidity and mortality. The aim of the study is to compare the sensitivity of two rapid diagnostic test kits in the diagnosis of malaria among patients attending Yobe State Specialist Hospital Damaturu. Patients were screened for malaria using blood samples collected in an EDTA container. Patients were tested with one Histidine rich protein-2(HRP-2) RDT (SD-Bioline) and a combination of Histidine rich protein-2(HRP-2) and Parasite lactose dehydrogenase enzyme (pLDH) RDT (CareStart). Microscopy was used as a gold standard. Out of 200 participants enrolled and screened for malaria, 200 (100%) tested positive by Microscopy, 159 (79.5%), tested positive by SD-Bioline (HRP 2 RDT) while 139 (69.5%), tested positive with the CareStart (pLDH RDT). Upon detection of the sensitivity by serially diluting the positive samples in other to decrease the parasite density; SD-Bioline was reactive at 1:4 dilution while CareStart was weekly reactive at this dilution. Although SD-Bioline showed to have a higher sensitivity than CareStart, both could be a suitable alternative to microscopy to screen endemic malaria in Nigeria.

Published in	International Journal of Infectious Diseases and Therapy (Volume 5, Issue 3)
DOI	10.11648/j.ijidt.20200503.21
Page(s)	99-105
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Diagnostic, Infection, Microscopy, Malaria, Yobe

References

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APA Style

Muktari Isa Shago, Markus Kirwe Jiyil, Nazifi Alhaji Sani, Garba Suleiman. (2020). Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. International Journal of Infectious Diseases and Therapy, 5(3), 99-105. https://doi.org/10.11648/j.ijidt.20200503.21

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ACS Style

Muktari Isa Shago; Markus Kirwe Jiyil; Nazifi Alhaji Sani; Garba Suleiman. Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. Int. J. Infect. Dis. Ther. 2020, 5(3), 99-105. doi: 10.11648/j.ijidt.20200503.21

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AMA Style

Muktari Isa Shago, Markus Kirwe Jiyil, Nazifi Alhaji Sani, Garba Suleiman. Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. Int J Infect Dis Ther. 2020;5(3):99-105. doi: 10.11648/j.ijidt.20200503.21

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@article{10.11648/j.ijidt.20200503.21,
  author = {Muktari Isa Shago and Markus Kirwe Jiyil and Nazifi Alhaji Sani and Garba Suleiman},
  title = {Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard},
  journal = {International Journal of Infectious Diseases and Therapy},
  volume = {5},
  number = {3},
  pages = {99-105},
  doi = {10.11648/j.ijidt.20200503.21},
  url = {https://doi.org/10.11648/j.ijidt.20200503.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijidt.20200503.21},
  abstract = {Malaria remains a public health problem in over 90 countries worldwide, especially in Africa where it is the cause of morbidity and mortality. The aim of the study is to compare the sensitivity of two rapid diagnostic test kits in the diagnosis of malaria among patients attending Yobe State Specialist Hospital Damaturu. Patients were screened for malaria using blood samples collected in an EDTA container. Patients were tested with one Histidine rich protein-2(HRP-2) RDT (SD-Bioline) and a combination of Histidine rich protein-2(HRP-2) and Parasite lactose dehydrogenase enzyme (pLDH) RDT (CareStart). Microscopy was used as a gold standard. Out of 200 participants enrolled and screened for malaria, 200 (100%) tested positive by Microscopy, 159 (79.5%), tested positive by SD-Bioline (HRP 2 RDT) while 139 (69.5%), tested positive with the CareStart (pLDH RDT). Upon detection of the sensitivity by serially diluting the positive samples in other to decrease the parasite density; SD-Bioline was reactive at 1:4 dilution while CareStart was weekly reactive at this dilution. Although SD-Bioline showed to have a higher sensitivity than CareStart, both could be a suitable alternative to microscopy to screen endemic malaria in Nigeria.},
 year = {2020}
}

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TY  - JOUR
T1  - Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard
AU  - Muktari Isa Shago
AU  - Markus Kirwe Jiyil
AU  - Nazifi Alhaji Sani
AU  - Garba Suleiman
Y1  - 2020/09/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijidt.20200503.21
DO  - 10.11648/j.ijidt.20200503.21
T2  - International Journal of Infectious Diseases and Therapy
JF  - International Journal of Infectious Diseases and Therapy
JO  - International Journal of Infectious Diseases and Therapy
SP  - 99
EP  - 105
PB  - Science Publishing Group
SN  - 2578-966X
UR  - https://doi.org/10.11648/j.ijidt.20200503.21
AB  - Malaria remains a public health problem in over 90 countries worldwide, especially in Africa where it is the cause of morbidity and mortality. The aim of the study is to compare the sensitivity of two rapid diagnostic test kits in the diagnosis of malaria among patients attending Yobe State Specialist Hospital Damaturu. Patients were screened for malaria using blood samples collected in an EDTA container. Patients were tested with one Histidine rich protein-2(HRP-2) RDT (SD-Bioline) and a combination of Histidine rich protein-2(HRP-2) and Parasite lactose dehydrogenase enzyme (pLDH) RDT (CareStart). Microscopy was used as a gold standard. Out of 200 participants enrolled and screened for malaria, 200 (100%) tested positive by Microscopy, 159 (79.5%), tested positive by SD-Bioline (HRP 2 RDT) while 139 (69.5%), tested positive with the CareStart (pLDH RDT). Upon detection of the sensitivity by serially diluting the positive samples in other to decrease the parasite density; SD-Bioline was reactive at 1:4 dilution while CareStart was weekly reactive at this dilution. Although SD-Bioline showed to have a higher sensitivity than CareStart, both could be a suitable alternative to microscopy to screen endemic malaria in Nigeria.
VL  - 5
IS  - 3
ER  -

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Author Information

Muktari Isa Shago

Department of Chemistry, Yobe State University Damaturu, Damaturu, Nigeria
Markus Kirwe Jiyil

Department of Biochemistry, University of Jos, Jos, Nigeria
Nazifi Alhaji Sani

Department of Chemistry, Yobe State University Damaturu, Damaturu, Nigeria
Garba Suleiman

Department of Chemistry, Yobe State University Damaturu, Damaturu, Nigeria

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APA Style

Muktari Isa Shago, Markus Kirwe Jiyil, Nazifi Alhaji Sani, Garba Suleiman. (2020). Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. International Journal of Infectious Diseases and Therapy, 5(3), 99-105. https://doi.org/10.11648/j.ijidt.20200503.21

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ACS Style

Muktari Isa Shago; Markus Kirwe Jiyil; Nazifi Alhaji Sani; Garba Suleiman. Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. Int. J. Infect. Dis. Ther. 2020, 5(3), 99-105. doi: 10.11648/j.ijidt.20200503.21

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AMA Style

Muktari Isa Shago, Markus Kirwe Jiyil, Nazifi Alhaji Sani, Garba Suleiman. Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard. Int J Infect Dis Ther. 2020;5(3):99-105. doi: 10.11648/j.ijidt.20200503.21

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@article{10.11648/j.ijidt.20200503.21,
  author = {Muktari Isa Shago and Markus Kirwe Jiyil and Nazifi Alhaji Sani and Garba Suleiman},
  title = {Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard},
  journal = {International Journal of Infectious Diseases and Therapy},
  volume = {5},
  number = {3},
  pages = {99-105},
  doi = {10.11648/j.ijidt.20200503.21},
  url = {https://doi.org/10.11648/j.ijidt.20200503.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijidt.20200503.21},
  abstract = {Malaria remains a public health problem in over 90 countries worldwide, especially in Africa where it is the cause of morbidity and mortality. The aim of the study is to compare the sensitivity of two rapid diagnostic test kits in the diagnosis of malaria among patients attending Yobe State Specialist Hospital Damaturu. Patients were screened for malaria using blood samples collected in an EDTA container. Patients were tested with one Histidine rich protein-2(HRP-2) RDT (SD-Bioline) and a combination of Histidine rich protein-2(HRP-2) and Parasite lactose dehydrogenase enzyme (pLDH) RDT (CareStart). Microscopy was used as a gold standard. Out of 200 participants enrolled and screened for malaria, 200 (100%) tested positive by Microscopy, 159 (79.5%), tested positive by SD-Bioline (HRP 2 RDT) while 139 (69.5%), tested positive with the CareStart (pLDH RDT). Upon detection of the sensitivity by serially diluting the positive samples in other to decrease the parasite density; SD-Bioline was reactive at 1:4 dilution while CareStart was weekly reactive at this dilution. Although SD-Bioline showed to have a higher sensitivity than CareStart, both could be a suitable alternative to microscopy to screen endemic malaria in Nigeria.},
 year = {2020}
}

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TY  - JOUR
T1  - Evaluation of the Sensitivity of Rapid Diagnostic Test (RDT) of Malaria in Different Age and Sex Groups Using Microscopy as a Gold Standard
AU  - Muktari Isa Shago
AU  - Markus Kirwe Jiyil
AU  - Nazifi Alhaji Sani
AU  - Garba Suleiman
Y1  - 2020/09/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijidt.20200503.21
DO  - 10.11648/j.ijidt.20200503.21
T2  - International Journal of Infectious Diseases and Therapy
JF  - International Journal of Infectious Diseases and Therapy
JO  - International Journal of Infectious Diseases and Therapy
SP  - 99
EP  - 105
PB  - Science Publishing Group
SN  - 2578-966X
UR  - https://doi.org/10.11648/j.ijidt.20200503.21
AB  - Malaria remains a public health problem in over 90 countries worldwide, especially in Africa where it is the cause of morbidity and mortality. The aim of the study is to compare the sensitivity of two rapid diagnostic test kits in the diagnosis of malaria among patients attending Yobe State Specialist Hospital Damaturu. Patients were screened for malaria using blood samples collected in an EDTA container. Patients were tested with one Histidine rich protein-2(HRP-2) RDT (SD-Bioline) and a combination of Histidine rich protein-2(HRP-2) and Parasite lactose dehydrogenase enzyme (pLDH) RDT (CareStart). Microscopy was used as a gold standard. Out of 200 participants enrolled and screened for malaria, 200 (100%) tested positive by Microscopy, 159 (79.5%), tested positive by SD-Bioline (HRP 2 RDT) while 139 (69.5%), tested positive with the CareStart (pLDH RDT). Upon detection of the sensitivity by serially diluting the positive samples in other to decrease the parasite density; SD-Bioline was reactive at 1:4 dilution while CareStart was weekly reactive at this dilution. Although SD-Bioline showed to have a higher sensitivity than CareStart, both could be a suitable alternative to microscopy to screen endemic malaria in Nigeria.
VL  - 5
IS  - 3
ER  -

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