Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum
International Journal of Chinese Medicine
Volume 4, Issue 1, March 2020, Pages: 1-11
Received: Nov. 28, 2019; Accepted: Dec. 18, 2019; Published: Jan. 21, 2020
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Authors
Yi Luo, Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Cejia Liu, Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Shengci Fan, Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Zhaoqing Meng, Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Fei He, Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
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Abstract
The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).
Keywords
QAMS, Fingerprint, Bufadienolides, Bufonis Venenum, Method
To cite this article
Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He, Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum, International Journal of Chinese Medicine. Vol. 4, No. 1, 2020, pp. 1-11. doi: 10.11648/j.ijcm.20200401.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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