International Journal of Chinese Medicine

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Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum

Received: 28 November 2019    Accepted: 18 December 2019    Published: 21 January 2020
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Abstract

The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).

DOI 10.11648/j.ijcm.20200401.11
Published in International Journal of Chinese Medicine (Volume 4, Issue 1, March 2020)
Page(s) 1-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

QAMS, Fingerprint, Bufadienolides, Bufonis Venenum, Method

References
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[5] Song, H., Guo, T., Bi, K., Wang, H., et al. Determination of resibufogenin and cinobufagin in heart-protecting musk pill by HPLC [J]. Bio Chroma 2000, 14, 130–132.
[6] Wu X. Y.; Gao H. M.; Wang Z. M. Advances on chemical constituents in crude medicines from toads [J]. Chin. J. Exp. Tradit. Med. Formulae 2010, 16, 207–214.
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[8] Chen Y. L., Hao Y. Y., Guo F. J., et al. Research progress on chemical constituents and pharmacological activities of Bufonis Venenum [J]. Chin. Tra. Her. Drug 2017, 12, 2579-2587.
[9] Wang Y. Study on chemical constituents of cardiac glycosides from Bufonis Venenum [D]. Jilin University 2015.
[10] Guillermo S. H., Cristina Q., Gabriel V. A., et al. Antiproliferative activity and chemical composition of the venom from the Amazonian toad Rhinella marina (Anura: Bufonidae) [J]. Toxicon 2016, 121, 119-129.
[11] Cui L. L., Zhang Y. Y., Shao W., et al. Analysis of the HPLC fingerprint and QAMS from Pyrrosia species [J]. Indus. Crop Pro 2016, 85, 29-37.
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[13] Kim S., Kim K. Y., Han C. S. et al. Simultaneous analysis of six major compounds in Osterici Radix and Notopterygii Rhizoma et Radix by HPLC and discrimination of their origins from chemical fingerprint analysis [J]. Arch. Pharm. Res. 2012, 35 (4), 691–699.
[14] D. L., Liang Y. Z., Xie P. S. et al. Quality control and discrimination of pericarpium citri reticulatae and pericarpium citri reticulatae viride based on high-performance liquid chromatographic fingerprints and multivariate statistical analysis. [J] Anal. Chim. Acta, 2007, 588 (2), 207–215.
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Author Information
  • Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

  • Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

  • Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

  • Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

  • Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

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    Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. (2020). Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. International Journal of Chinese Medicine, 4(1), 1-11. https://doi.org/10.11648/j.ijcm.20200401.11

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    Yi Luo; Cejia Liu; Shengci Fan; Zhaoqing Meng; Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int. J. Chin. Med. 2020, 4(1), 1-11. doi: 10.11648/j.ijcm.20200401.11

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    AMA Style

    Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int J Chin Med. 2020;4(1):1-11. doi: 10.11648/j.ijcm.20200401.11

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  • @article{10.11648/j.ijcm.20200401.11,
      author = {Yi Luo and Cejia Liu and Shengci Fan and Zhaoqing Meng and Fei He},
      title = {Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum},
      journal = {International Journal of Chinese Medicine},
      volume = {4},
      number = {1},
      pages = {1-11},
      doi = {10.11648/j.ijcm.20200401.11},
      url = {https://doi.org/10.11648/j.ijcm.20200401.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijcm.20200401.11},
      abstract = {The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum
    AU  - Yi Luo
    AU  - Cejia Liu
    AU  - Shengci Fan
    AU  - Zhaoqing Meng
    AU  - Fei He
    Y1  - 2020/01/21
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijcm.20200401.11
    DO  - 10.11648/j.ijcm.20200401.11
    T2  - International Journal of Chinese Medicine
    JF  - International Journal of Chinese Medicine
    JO  - International Journal of Chinese Medicine
    SP  - 1
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2578-9473
    UR  - https://doi.org/10.11648/j.ijcm.20200401.11
    AB  - The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).
    VL  - 4
    IS  - 1
    ER  - 

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