Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi

doi:doi:10.11648/j.wjph.20190404.14

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Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi

Published in World Journal of Public Health (Volume 4, Issue 4)

Received: 9 May 2018 Accepted: 24 May 2018 Published: 10 December 2019

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Abstract

Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 10⁹/l to 14.0±0.216x 10⁹/l, 11.2±0.109x10⁹/l and 12.3±0.148 x10⁹/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x10³/ul to 529±0.01x 10³/ul, 332±0.523x10³/ul and 135±0.543x10³/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.

Published in	World Journal of Public Health (Volume 4, Issue 4)
DOI	10.11648/j.wjph.20190404.14
Page(s)	96-101
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Achyranthes Aspera, Hematological Parameter, Albino Mice

References

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APA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. (2019). Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World Journal of Public Health, 4(4), 96-101. https://doi.org/10.11648/j.wjph.20190404.14

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ACS Style

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J. Public Health 2019, 4(4), 96-101. doi: 10.11648/j.wjph.20190404.14

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AMA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J Public Health. 2019;4(4):96-101. doi: 10.11648/j.wjph.20190404.14

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@article{10.11648/j.wjph.20190404.14,
  author = {Emmanuel Asuquo Etim and Yusuf Abdulhakeem Adebayo and Obeagu Emmanuel Ifeanyi},
  title = {Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice},
  journal = {World Journal of Public Health},
  volume = {4},
  number = {4},
  pages = {96-101},
  doi = {10.11648/j.wjph.20190404.14},
  url = {https://doi.org/10.11648/j.wjph.20190404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20190404.14},
  abstract = {Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.},
 year = {2019}
}

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TY  - JOUR
T1  - Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice
AU  - Emmanuel Asuquo Etim
AU  - Yusuf Abdulhakeem Adebayo
AU  - Obeagu Emmanuel Ifeanyi
Y1  - 2019/12/10
PY  - 2019
N1  - https://doi.org/10.11648/j.wjph.20190404.14
DO  - 10.11648/j.wjph.20190404.14
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 96
EP  - 101
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20190404.14
AB  - Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.
VL  - 4
IS  - 4
ER  -

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Author Information

Emmanuel Asuquo Etim

Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria
Yusuf Abdulhakeem Adebayo

Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria
Obeagu Emmanuel Ifeanyi

Department of University Health Services, Micheal Okpara University, Abia State, Nigeria

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APA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. (2019). Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World Journal of Public Health, 4(4), 96-101. https://doi.org/10.11648/j.wjph.20190404.14

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ACS Style

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J. Public Health 2019, 4(4), 96-101. doi: 10.11648/j.wjph.20190404.14

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AMA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J Public Health. 2019;4(4):96-101. doi: 10.11648/j.wjph.20190404.14

Copy | Download

@article{10.11648/j.wjph.20190404.14,
  author = {Emmanuel Asuquo Etim and Yusuf Abdulhakeem Adebayo and Obeagu Emmanuel Ifeanyi},
  title = {Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice},
  journal = {World Journal of Public Health},
  volume = {4},
  number = {4},
  pages = {96-101},
  doi = {10.11648/j.wjph.20190404.14},
  url = {https://doi.org/10.11648/j.wjph.20190404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20190404.14},
  abstract = {Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice
AU  - Emmanuel Asuquo Etim
AU  - Yusuf Abdulhakeem Adebayo
AU  - Obeagu Emmanuel Ifeanyi
Y1  - 2019/12/10
PY  - 2019
N1  - https://doi.org/10.11648/j.wjph.20190404.14
DO  - 10.11648/j.wjph.20190404.14
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 96
EP  - 101
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20190404.14
AB  - Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.
VL  - 4
IS  - 4
ER  -

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