Co-exposure of Lead Acetate and Sodium Arsenite Causes Alteration in the Markers of Liver and Kidney Functions in Male Wistar Rats
Journal of Chemical, Environmental and Biological Engineering
Volume 2, Issue 1, June 2018, Pages: 32-39
Received: Jul. 5, 2018; Accepted: Jul. 27, 2018; Published: Aug. 27, 2018
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Omowumi Oyeronke Adewale, Department of Biochemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria
Ekundayo Stephen Samuel, Cancer Research and Molecular Biology Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria
Opeyemi Olusayo Oluwuyi, Department of Biochemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria
Seun Funmilola Akomolafe, Department of Biochemistry, Faculty of Science, Ekiti State University, Ado Ekiti, Nigeria
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Study aim: More is still to be known about the combinative effect of lead and arsenic compounds on critical organs. In this study, the effect of single and combined exposure to lead and arsenic on some biomarkers associated with liver and kidney functions in healthy Wistar rats was assessed. Method: The rats were divided into four groups (n = 5) and were treated with sodium arsenite or lead acetate individually or in combination for 14 days. Results: The results revealed that single exposure to either compound caused significant increase in the hepatic transaminases and alkaline phosphatase. Significant decrease in serum proteins and glucose concentration were also observed with morphological changes in the liver of treated rats as discovered by the photomicrographs from light microscopy indicating hepatotoxicity. Similarly, significant increase in the blood urea nitrogen (BUN) and creatinine concentration with simultaneous rise in the concentrations of serum potassium and sodium were observed. The photomicrographs of the kidney from light microscopy showed congestion in the interstitial spaces indicating compromised function of the kidney. The combination of the two metals demonstrated the enhanced effect on these parameters when likened with their individual treatments. Conclusion: This study therefore proves the enhanced toxicity induced by co-exposure to lead acetate and sodium arsenite among biomarkers of liver and kidney functions in Wistar rats.
Lead Acetate, Sodium Arsenite, Liver Biomarkers, Kidney Biomarkers, Histopathology and Toxicity
To cite this article
Omowumi Oyeronke Adewale, Ekundayo Stephen Samuel, Opeyemi Olusayo Oluwuyi, Seun Funmilola Akomolafe, Co-exposure of Lead Acetate and Sodium Arsenite Causes Alteration in the Markers of Liver and Kidney Functions in Male Wistar Rats, Journal of Chemical, Environmental and Biological Engineering. Vol. 2, No. 1, 2018, pp. 32-39. doi: 10.11648/j.jcebe.20180201.16
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Mahaffay KK. (1990). Introduction: Advances in lead research: Implications for environmental health. Environ. Health perpect. 89: 95-100.
Parvez F, Chen Y, Argos M, Hussain AZ, Momotaj H, Dhar R, van Geen A, Graziano JH, Ahsan H. (2006). Prevalence of arsenic exposure from drinking water and awareness of its health risks in a Bangladeshi population; results from a large population-based study. Environ. Health Perspect. 114(3):355-59.
Omiecinski CJ, John P, Vanden H, Gary H. Perdew, Jeffrey M.P. (2011). “Xenobiotic Metabolism, Disposition, and Regulation by Receptors: From Biochemical Phenomenon to Predictors of Major Toxicities.” Toxicol Sci. 120 (SUPPL. 1). https://doi. org/10.1093 /toxsci /kfq338.
Malaguarnera G, Emanuela C, Maria G, et al. (2012) “Toxic Hepatitis in Occupational Exposure to Solvents.” World Journal of Gastroenterology 18 (22): 2756–66.
Durham AE, Smith KC, Newton JR. (2003). An evaluation of diagnostic data in comparison to the results of liver biopsies in mature horses. Equine Vet J. 35(6):554-9.
Kim WR, Steven LF, Adrian MD, Adrian MD, Henry CB. (2008). “Serum Activity of Alanine Aminotransferase (ALT) as an Indicator of Health and Disease.” J Hepatol. https: //doi. Org/10.1002/hep.22109.
Mogg TD, Palmer JE. (1995). Hyperlipidemia, hyperlipemia, and hepatic lipidosis in American Miniature Horses: 23 cases (1990–1994). J Am Vet Med Assoc. 207: 604–607.
West HJ. (1994). The evaluation of hepatobiliary disease in horses and cattle. FRCVS Thesis, London.
Offor SJ, Mbagwu HOC, Orisakwe OE. (2017). Lead Induced Hepato-Renal Damage in Male Albino Rats and Effects of Activated Charcoal. Front Pharmacol
National Agency for Food and Drug Administration and Control (2006) available at: www.nafdacni
Nehez M, Lorencz R, Desi I. (2000). Simultaneous action of cypermethrin and two environmental pollutant metals, cadmium and lead, on bone marrow cell chromosomes of rats in subchronic administration. Ecotoxicol Environ Saf. 45: 55-60.
Englyst V, Lundstrom NG, Gerhandsson L, Rylander L, Nordberg G. (2001). Lung cancer risks among lead smelter workers also exposed to arsenic. Sci. Total Environ. 273(1-3):77-82.
Lambert TW, Lane S,. (2004). “Lead, Arsenic, and Polycyclic Aromatic Hydrocarbons in Soil and House Dust in the Communities Surrounding the Sydney, Nova Scotia, Tar Ponds.” Environ. Health perpect. 112 (1): 35–41.
Odunola OA, Akinwumi KA,; Ogunbiyi B, Tugbobo O. (2007). Interaction and Enhancement of the Toxic Effects of Sodium Arsenite and Lead Acetate in Wistar Rats. Afr J Biomed Res. 10; 59 – 65.
Sujatha K, Srilatha C, Anjaneyulu Y, Amaravathi P. (2011). “Lead acetate induced neurotoxicity in wistar albino rats: A pathological, immunological, and ultrastructural studies.” Journal of pharma and bio science. 2: 459-62.
Escribano A, Revilla M, Hernandez ER, Seco C, Gonzalez-Riola J, Villa LF, Rico H. (1997). Effect of lead on bone development and bone mass: a morphometric, densitometric and histomorphometric study in growing rats. Calcif. Tissue Int. 60: 200-203.
Reitman S, Frankel S. (1957). A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28 56–62.
Szasz G. (1969). A kinetic photometric method for serum gamma glutamyl transferase. Clin Chem. 124: 124-136.
Englehardt A. (1970). Measurement of alkaline phosphatase. Aerztl Labor 16:42. 1
Gornal AG, Barawill JC, David MM. (1949). Determination of serum protein by means of Biuret reaction J Biol Chem. 177: 751-761.
Grant GH. (1987). Amino acids and protein; fundamentals of clinical chemistry. In: Tietz NW (ed) WB Saunders company Philadelphia USA: pp. 328-329.
Bucolo G. and David H. (1973). Quantitative Determination of Serum Triglycerides by Use of Enzymes. Clinical Chemistry, 19, 476-482.
Trinder P. (1969). Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromagen. J Clin Pathol. 22:158–161.
Weatherburn MW. (1967). Phenol hypochlorite reaction for determination of serum urea. Analyt. Chem. 39 (8):939-971.
Henry RF. (1974). Clinical Chemistry Principle and Techniques. 2nd edition, published by Harper and Row, New York.
Maruna RFL, Trinder P. (1958). Analysis of serum sodium and potassium ions. Clin Chim Acta. 2: 581-585.
Tchounwou PB, Clement GY, Anita KP, Dwayne JS. (2012). “Heavy Metals Toxicity and the Environment.” In Molecular, Clinical and Environmental Toxicology. 1007/978-3-7643-8340-4_6.
Orisakwe OE. (2014). “Lead and Cadmium in Public Health in Nigeria Physicians Neglect and Pitfall in Patient Management. North American Journal of Med Sci. 6(2): 61–70.
Hu B, Xiaolin J, Jie H, Dongyun X, Fang X, Yan L. (2017). “Assessment of Heavy Metal Pollution and Health Risks in the Soil-Plant-Human System in the Yangtze River Delta, China.” Int J Environ Res Public Health 14(9).
Elias RW. (1985). Lead exposure in human environment. In: Mahaffey K Dietary and environmental lead: Human health effects. Elsevier Amsterdarm-New York-Oxford. pp 79-107.
Abernathy CO, Liu YP, Long-Fellow D, Aposhian HV, Beck B, Fowler B, Goyer R, Menzer R, Rossman T, Thompson C. Waalkes M. (1999). Arsenic: Health effects, Mechanism of actions and research issue. Environ Health Perspect. 107: 593-597.
Poma A, Pittalugal E, Tucci A. (2003). Lead acetate genotoxicity on human melanoma cells in vitro. Melanoma Res. 13(6): 563-566.
Concepción NM, Pilar MM, Martín A, Jiménez J, Pilar UM. (1993). Free radical scavenger and antihepatotoxic activity of Rosmarinus tomentosus. Planta Med. 59(4):312-4.
Klibet F, Amel B, Mohamed K, Abdelfattah E, Cherif A, Mahfoud M. (2016). “Oxidative Stress-Related Liver Dysfunction by Sodium Arsenite: Alleviation by Pistacia Lentiscus Oil.” Pharm Biol.
De Smet R, Van Kaer J, Van Vlem B, De Cubber A, Brunet P, Lameire N, Vanholder R. (2003). “Toxicity of Free P-Cresol: A Prospective and Cross-Sectional Analysis.” Clin Chem. 49(3): 470–78.
Tarhoni MH, Timothy L, David ER, Wayne GC. (2008). “Albumin Binding as a Potential Biomarker of Exposure to Moderately Low Levels of Organophosphorus Biomarkers.
Mitchell HR, Kline W. (2006). Core curriculum in nephrology, Renal Function Testing. Am J Kidney Dis. 47:174–183.
Pagana KD. (1998). Mosby's Manual of Diagnostic and Laboratory Tests. St. Louis Mosby, Inc.
Edmund L, David J. (2006). Kidney function tests. In: Carl AB, Edward R, David E, editors. Tietz Textbook of clinical chemistry and molecular diagnostics. 4th ed. New Delhi: Elsevier Inc; pp. 797–808.
James S, Mitchel G. (2006). Physiology and disorder of water electrolytes and acid base metabo-lism. In: Carl AB, Edward R, David E, editors. Tietz Textbook of clinical chemistry and molecular diagnostics. 4th ed. New Delhi: Elsevier Inc;. pp. 1747–1776.
Calabrese EJ, Baldwin LA. (2008). Lead-Induced Cell Proliferation and Organ-Specific Tumori-genicity. Drug Metab Rev. 24:3.
Garu U., Sharma R. and Barber I. (2011). Effect of lead toxicity on developing testis of mice. IJPSR.0975- 8232.2(9).2403-07
Assi MA., Abba Y, Abdulkhaleq LA. et al. (2018). Effect of powdered seed of Nigella sativa administration on sub-chronic and chronic lead acetate induced hemato- biochemical and histopathological changes in Sprague Dawley rats. Comp Clin Pathol (2018) 27: 705.
Jegede AI., Ajadi M., Akinloye O. (2013). Modulatory effects of Kolaviron (Garcina kola extract) on spermogram and reproductive system of adult male Wistar rats in lead acetate induced toxicity. J. Toxicol Environ Sci.:5(7);121-130.
Dikhil M, Al-Khalifa M, Al-Quraishy S, Zrieq R, Moneim A (2016). Indigofera longifolia mitigates lead-acetate-induced kidney damage and apoptosis in a rat model. Drug Des, Dev. Therapy 10:1847-1856.
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