Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons
Journal of Chemical, Environmental and Biological Engineering
Volume 2, Issue 2, December 2018, Pages: 44-51
Received: Oct. 12, 2018; Accepted: Oct. 26, 2018; Published: Nov. 16, 2018
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Authors
Safa Bejaoui, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Khaoula Telahigue, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Imene Chetoui, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Imen Rabeh, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Chaima Fouzai, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Wafa Trabelsi, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Ines Houas-Gharsallah, Higher Institute of Marine Sciences and Technology, La Goulette Center, Tunis, Tunisia
M’hamed El Cafsi, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Nejla Soudani, Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
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Abstract
The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.
Keywords
Antioxidants Defense, Bioaccumulation, Calms, DNA Damage, Gills, Lagoons
To cite this article
Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, Wafa Trabelsi, Ines Houas-Gharsallah, M’hamed El Cafsi, Nejla Soudani, Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons, Journal of Chemical, Environmental and Biological Engineering. Vol. 2, No. 2, 2018, pp. 44-51. doi: 10.11648/j.jcebe.20180202.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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