The Inoculation with an Effecient AMF Strain Decreases the Phosphoric Fertilizer Requirements in Ipomea Batata (L), Lam in Dry Period
Journal of Chemical, Environmental and Biological Engineering
Volume 3, Issue 2, December 2019, Pages: 13-18
Received: Feb. 15, 2019; Accepted: Mar. 22, 2019; Published: Oct. 17, 2019
Views 502      Downloads 122
Alberto Espinosa Cuéllar, Institute of Tropical Crops Research (INIVIT), Santo Domingo, Villa Clara, Cuba
Luís Ruiz Martinez, Institute of Tropical Crops Research (INIVIT), Santo Domingo, Villa Clara, Cuba
Ramón Rivera Espinosa, National Institute of Agricultural Sciences (INCA), San José de las Lajas, Mayabeque, Cuba
Ernesto Espinosa Cuéllar, Institute of Tropical Crops Research (INIVIT), Santo Domingo, Villa Clara, Cuba
Article Tools
Follow on us
While Ipomeabatata (L), Lam. is a mycotic crop, few results are reported in establishing the requirements of phosphoric fertilizers associated with the inoculation of arbuscular mycorrhizal fungus (AMF) strains. For these purposes, two experiments were carried out, evaluating the response of 'INIVIT B2-2005' or 'CEMSA 78-354'clones to the application of five doses of phosphoric fertilizer with or without the application of Rhizoglomu sirregulare/(INCAM-11) species on a carbonated loose Brown soil, at a random block design with factorial arrangement and four repetitions. The experiments were carried out with 'INIVIT B2-2005' and 'CEMSA 78-354'clones always in the period November - April and they were carried out in two years. The yield of tubers, percentage of mycorrhizal colonization, content of mycorrhizal spores in 50 grams of soil and phosphorus content in the leaf, stem and tuberous root were evaluated. A significant response (p <0.05) to mineral fertilization was found in the yield with an optimum dose of 75 kg ha-1 which decreased to 50 kg ha-1 when inoculated with R. irregulare, keeping equivalent yields. This effect was similar in both clones and in both years. The inoculation increased the colonization percentage and the spores significantly, and the highest values were always obtained in the treatment of 50 kg ha-1 of P2O5, lower and higher fertilizations had lower values. The phosphorus contents in different organs also presented a similar response. It is concluded that the inoculation of an efficient AMF strain causes an adequate mycorrhizal functioning in presence of medium doses of fertilizers, guaranteeing high yields with lower doses of these ones.
Colonization, Doses, Fertilizations, Spores, Yield
To cite this article
Alberto Espinosa Cuéllar, Luís Ruiz Martinez, Ramón Rivera Espinosa, Ernesto Espinosa Cuéllar, The Inoculation with an Effecient AMF Strain Decreases the Phosphoric Fertilizer Requirements in Ipomea Batata (L), Lam in Dry Period, Journal of Chemical, Environmental and Biological Engineering. Vol. 3, No. 2, 2019, pp. 13-18. doi: 10.11648/j.jcebe.20190302.11
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
El–Sayed, H. E. A, A. S. El-Dean, S. Ezzat, and A. H. A. El–Morsy. Responses of productivity and quality of sweet potato to phosphorus fertilizer rate and application methods of the humicacid. Int. Res. J. Agric. Sci. Soil Sci., 1: 383-393. 2011.
Fernández, F.; Gómez, R.; Martínez, M. A.; Noval, B. M. de la.; Rivera R. Mycorrhizal inoculant product. Patent No. 22 641. Cuba, 2000.
Gerdemann, J. W.; Nicholson, T. H. Spore of mycorrhizaeendogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. vol. 46, p. 235-244. 1963.
González, P. J.; Ramírez, J. F.; Morgan, O.; Rivera, R.; Plana, R. Contribution of arbuscularmycorrhizal inoculation to the reduction of phosphorus fertilization in Brachiariadecumbens. Tropical Crops, vol. 36, no. 1, pp. 135-142 January-March. Digital ISSN: 1819-4087.2015.
Hernández, J. A.; Pérez, J. J. M.; Bosch, I. D. and Castro, S. N. Classification of the soils of Cuba. Editions INCA, Cuba, 93 p. ISBN: 978-959-7023-77-7.2015.
Herrera, R. A .; Ferrer, R. L .; Furrazola, E .; Orozco, M. O. Strategy for the operation of VA mycorrhizae in a tropical forest. Biodiversity in Latin America. Ecosystems, evolution and social processes. Mérida, Mexico: Ibero-American Program of Science and Technology for Development. Subprogram XII, Biological Diversity. 1995.
Hodge A, Fitter A . Substantial nitrogen acquisition by arbuscularmycorrhizal fungi from organic material has implications for N cycling. Proceedings of the National Academy of Sciences USA107: 13754–13759. 2010.
H. S. Abdel-Razzak, A. G. Moussa, M. A. Abd El-Fattah and G. A. El-Morabet. Response of Sweet Potato to Integrated Effect of Chemical and Natural Phosphorus Fertilizer and Their Levels in Combination with Mycorrhizal Inoculation. Journal of Biological Sciences, 13: 112-122. 2013.
INSMET. Institute of Meteorology. Settling sheets of the daily meteorological variables. Weather station No.326, Santo Domingo, Villa Clara, Cuba. 2013.
Jeffries P, Gianinazzi S, Perotto S, Turnau K, Barea JM. The contribution of arbuscularmycorrhizal fungi in sustainable maintenance of plant health andsoil fertility. Biol. Fértil. Soils 37: 1–16.2003.
oão, J. P.; Espinosa, A; Ruíz, L; Simó, Jy Rivera R. Effectiveness of strains of AMF in the cultivation of cassava (Manihotesculentacrantz) in two soil types. Tropical Crops, vol. 37, no. 1, pp. 48-56. ISSN 0258-5936. 2016.
Liu A, Plenchette C, Hamel C . Soil nutrient and water providers: how arbuscularmycorrhizal mycelia support plant performance in a resource limited world. In: Hamel C, Plenchette C (eds) Mycorrhizae in crop production. Haworth, Binghampton, pp 37–66.2007.
MINAG. Technical instructions on the cultivation of sweet potatoes. Ministry of Agriculture. SEDARI / AGINFOR. City of Havana, Cuba. 24 pp. 2011.
NRAG 564. Plant analysis. Foliar analysis. Test methods. NRAG 564: 82. City of Havana: MINAG.13p.1982.
Phillips, J. M. y Hayman, D. E. Improved procedures for clearing groots and staining parasitic and vesicular-arbuscularmycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc., vol. 55, p. 158-161. 1970.
Ruiz, L. Effectiveness of mycorrhizal associations in roots and tubers in two soil types. [Online PhD thesis]. Havana: Agrarian University of Havana (UNAH). National Institute of Agricultural Sciences (INCA) .101p. [In line]. [Accessed: April 2014]. Available at: .2001.
Rivera, R. and Fernández, Kalyanne. Scientific-technical bases for the efficient management of agricultural systems mycorrhized. In: Efficient management of mycorrhizal symbiosis, a path towards sustainable agriculture. Case study: the Caribbean. Havana: National Institute of Agricultural Sciences (INCA). p. 51-94. ISBN: 959-7023-24-5. 2003.
Rivera, R.; Fernández, F.; Fernández, Kalyanne, Ruiz, L.; Sánchez, C.; Riera, M. Advances in the management of effective arbuscularmycorrhizal symbiosis in tropical ecosystems. In: Hamel C., Plenchette C. (Eds.) Mycorrhizae in Crop Production. Haworth Press, Binghamton, N. Y., pp. 151-196. Hard Cover ISBN: 978-1-56022-306-1; Soft Cover ISBN: 978-1-56022-307-8.2007.
Rivera, R.; González, P. J.; Hernández, A.; Martín, Gloria, Ruiz, L.; Fernández, Kalyanne, Simó, J.; García, Milagro, Pérez, A.; Riera, M.; Bustamante, C.; Joao, J. P. and Ruiz, M. The importance of soil environment and pH on the effectiveness and recommendation of efficient strains of AMF for the inoculation of crops. [CD-Rom] In: VIII Congress of the Cuban Society of Soil Science. (June 2 to 5, 2015) Memories. Havana Cuba. ISBN 978-959-296-039-8. 2015.
Ruiz, L; J. Simo and R. Rivera. Mycorrhizae in tropical crops. A contribution to agri-food sustainability. - Madrid: Editorial Académica Española, ISBN: 978-3-8484-5382-5, 2012. - 239 p. 2012.
Simó González J .; L. Ruiz Martinez, R. Rivera Espinosa. Management of the arbuscularmycorrhizal symbiosis and nutrient supply in plantations of banana cultivar 'FHIA-18' on soft carbonated Brown soils. Tropical Crops, vol. 36 no. 4, pp. 43-54. ISSN 0258-5936.2015.
Smith SE, Smith FA, Jakobsen I . Mycorrhizal fungi can dominate phosphate supply to plants irrespective of growth response. Plant Physiology 133: 16–20. 2003.
Smith SE, Read DJ. Mycorrhizal symbiosis (Harcourt Brace, San Diego). 2008.
Smith, S. E., and F. A. Smith.. Roles of arbuscularmycorrhizas in plant nutrition and growth: New paradigms from cellular to ecosystem scales. Ann. Rev. Plant Biol. 62: 227–50. doi: 10.1146/annurev-arplant-042110-103846.2011.
SPSS. Statistical Package for the Social Sciences. Version 2.1. Manual of the user of the basic IBM system. [Online] [Consulted: April 2014]. Available at:
Villegas, R., V Gómez, A. Matos and M. López. The phosphorus in the agriculture of Cuba. Presentations of the first National Meeting of Agrochemistry. Havana. ACC.69 p. 1983.
WRB (World reference base for soil resources). International soil classification system fornaming soils and creating legends for soil maps. World Soil Reports no. 106. FAO, Roma, 81 p. ISBN: 978-92-5-108369-7. ISSN: 0532-0488. 2014.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186