Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria
Journal of Chemical, Environmental and Biological Engineering
Volume 4, Issue 2, December 2020, Pages: 32-38
Received: Jan. 15, 2020; Accepted: Feb. 4, 2020; Published: Jun. 8, 2020
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Mfoniso Uko, Department of Microbiology, Faculty of Biological Science, Akwa Ibom State University, Ikot Akpaden, Nigeria
Ime Udotong, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria
Utibe Ofon, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria
Senyene Umana, Department of Microbiology, Faculty of Biological Science, Akwa Ibom State University, Ikot Akpaden, Nigeria
Nsikak Abraham, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria; Environmental Microbiology and Biotechnology Unit, International Centre for Energy and Environmental Sustainability Research, University of Uyo, Uyo, Nigeria
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A microbial composition study of sediments of contaminated (CWS) and uncontaminated (UWS) lentic ecosystems within Akwa Ibom State was carried out by analyzing the small-subunit rRNA genes to determine the effect of hydrocarbon contamination on its microbial composition and diversity. Analysis of the V4 region of the community DNA from both sediments revealed the presence of bacteria, archaea and microalgae. Bacterial sequences outnumbered archaea and microalgae. Abundance of Proteobacteria, Betaproteobacteria, Burholderiales, Alcaligenaceae, and Achrombacter were observed in the CWS and Actinobacteria, Actinomycetales, Bacillaceae, and Bacillus in the UWS. Crenarchaeota and Euryarchaeota were also observed to be present in both sediments. The genus Achromobacter and Bacillus dominated in the CWS and UWS, respectively. Uncultured bacterium with the accession number DQ404672.1 and AY917600.1 led at the species levels. Achromobacter sp.-AM232721.1 outnumbered the other species in the CWS such as Kitasatospora sp.-AF131379.1, Mycobacterium celatum-AF547908.1, Paenibacillus phyllosphaerae-NR_043008.1, Cystobacter fuscus-M94276.1, Planosporangium flavigriseum-NR_042508.1, etc. In the UWS, the dominant species was Bacillus sp.-AJ316313.1. Microalgae, Chlorella sp. and Chlorella vulgaris were also detected in both ecosystems. Diverse and distinct diversity of bacteria, archaea and microalgae are present in the sediments and only a few of them have cultured counterpart. The variation in the microbial communities from the two sites has revealed the impact of contaminants especially hydrocarbons on the microbial diversity in lentic ecosystems.
Freshwater, Sediment, Hydrocarbon, Prokaryotes, Microalgae, Culture-Independent
To cite this article
Mfoniso Uko, Ime Udotong, Utibe Ofon, Senyene Umana, Nsikak Abraham, Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria, Journal of Chemical, Environmental and Biological Engineering. Vol. 4, No. 2, 2020, pp. 32-38. doi: 10.11648/j.jcebe.20200402.11
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