Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria
Journal of Chemical, Environmental and Biological Engineering
Volume 4, Issue 2, December 2020, Pages: 32-38
Received: Jan. 15, 2020;
Accepted: Feb. 4, 2020;
Published: Jun. 8, 2020
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Mfoniso Uko, Department of Microbiology, Faculty of Biological Science, Akwa Ibom State University, Ikot Akpaden, Nigeria
Ime Udotong, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria
Utibe Ofon, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria
Senyene Umana, Department of Microbiology, Faculty of Biological Science, Akwa Ibom State University, Ikot Akpaden, Nigeria
Nsikak Abraham, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria; Environmental Microbiology and Biotechnology Unit, International Centre for Energy and Environmental Sustainability Research, University of Uyo, Uyo, Nigeria
A microbial composition study of sediments of contaminated (CWS) and uncontaminated (UWS) lentic ecosystems within Akwa Ibom State was carried out by analyzing the small-subunit rRNA genes to determine the effect of hydrocarbon contamination on its microbial composition and diversity. Analysis of the V4 region of the community DNA from both sediments revealed the presence of bacteria, archaea and microalgae. Bacterial sequences outnumbered archaea and microalgae. Abundance of Proteobacteria, Betaproteobacteria, Burholderiales, Alcaligenaceae, and Achrombacter were observed in the CWS and Actinobacteria, Actinomycetales, Bacillaceae, and Bacillus in the UWS. Crenarchaeota and Euryarchaeota were also observed to be present in both sediments. The genus Achromobacter and Bacillus dominated in the CWS and UWS, respectively. Uncultured bacterium with the accession number DQ404672.1 and AY917600.1 led at the species levels. Achromobacter sp.-AM232721.1 outnumbered the other species in the CWS such as Kitasatospora sp.-AF131379.1, Mycobacterium celatum-AF547908.1, Paenibacillus phyllosphaerae-NR_043008.1, Cystobacter fuscus-M94276.1, Planosporangium flavigriseum-NR_042508.1, etc. In the UWS, the dominant species was Bacillus sp.-AJ316313.1. Microalgae, Chlorella sp. and Chlorella vulgaris were also detected in both ecosystems. Diverse and distinct diversity of bacteria, archaea and microalgae are present in the sediments and only a few of them have cultured counterpart. The variation in the microbial communities from the two sites has revealed the impact of contaminants especially hydrocarbons on the microbial diversity in lentic ecosystems.
Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria, Journal of Chemical, Environmental and Biological Engineering.
Vol. 4, No. 2,
2020, pp. 32-38.
Abraham, N. A., Offiong, N. O., Ukafia, O. P. and Akpan, P. E. (2019). Source Apportionment of Polycyclic Aromatic Hydrocarbons (PAHs) in a Tropical Estuarine Epipelic Sediment and Its Associated Bacterial Degrading Potentials. Current Journal of Applied Science and Technology, 32 (1): 1-11.
Ezekiel, E. N., Hart, A. I. and Abowei, J. F. N. (2011). The sediment physical and chemical characteristics in Sombreiro River, Niger Delta, Nigeria. Research Journal of Environmental and Earth Sciences, 3 (4), 341-349.
Hassanshahian, M. (2014). The effects of crude oil on marine microbial communities in sediments from the Persian Gulf and the Caspian Sea: A microcosm experiment. International Journal of Advanced Biological and Biomedical Research, 2 (1): 1-17.
Rockne KJ, Shor LM, Young LY, Taghon GL, Kosson DS (2002) Distributed Sequestration and Release of PAHS in Weathered Sediment: The Role of Sediment Structure and Organic Carbon Properties. Environmental Science and Technology, 36: 2636–2644.
Hara, A., Syutsubo, K. and Harayama, S. (2003). Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation. Environmental Microbiology, 5 (9): 746–753.
Ghanavati, H., Emtiazi, G. and Hassanshahian, M. (2008). Synergism effects of phenol degrading yeast and Ammonia Oxidizing Bacteria for nitrification in coke wastewater of Esfahan Steel Company. Waste Management Resource, 26: 203-208.
Labud, V., Garcia, C. and Hernandez, T. (2007). Effect of hydrocarbon pollution on the microbial properties of a sandy and a clay soil. Chemosphere, 66: 1863–1871.
Margesin, R., Labbe, D., Schinner, F. C., Greer, W. and Whyte, L. G. (2003) Characterization of hydrocarbon-degrading microbial populations in contaminated and pristine alpine soils. Applied Environmental Microbiology, 69: 3085–3092.
Cappello, S., Caruso, G., Zampino, D., Monticelli, L. S., Maimone, G., Denaro, R., Tripodo, B., Troussellier, M., Yakimov, M. M. and Giuliano, L. (2007). Microbial community dynamics during assays of harbour oil spill bioremediation: a microscale simulation study. Journal of Applied Microbiology, 102 (1): 184-194.
Amann, R. I., Ludwig, W. and Schleifer, K. H. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiology Reviews, 59: 143–169.
Zhang, J., Ding, X., Guan, R., Zhu, C., Xu, C., Zhu, B., Zhang, H., Xiong, Z., Xue, Y., Tu, J. and Lu, Z. (2018). Evaluation of different 16S rRNA gene V regions for exploring bacterial diversity in a eutrophic freshwater lake. Science of Total Environment, 618: 1254-1267.
Udotong, I. R., Uko, M. P. and Udotong, J. I. R. (2015). Microbial diversity of a remote aviation fuel-contaminated sediment of a lentic ecosystem in Ibeno, Nigeria. Journal of Environmental and Analytical Toxicology, 5: 320.
Diaz, R. J. and Rosenberg, R. (2008). Spreading dead zones and consequences for marine ecosystems. Science 321: 926–929.
Urakawa, H., Martens-Habbena, W. and Stahl, D. A. (2010). High abundance of ammonia-oxidizing Archaea in coastal waters, determined using a modified DNA extraction method. Applied and Environmental Microbiology, 76: 2129–35.
Pollet, T., Tadonléké, R. D. and Humbert, J. F. (2011). Comparison of primer sets for the study of Planctomycetes communities in lentic freshwater ecosystems. Environmental Microbiology Reports, 3 (2) 254-261.
Buckley, D. H., Huangyutitham, V., Nelson, T. A., Rumberger, A., Thies, J. E. (2006). Diversity of planctomycetes in soil in relation to soil history and environmental heterogeneity. Applied and Environmental Microbiology, 72 (7), 4522-4531.
Bucci, J. P., Szempruch, A. J., Caldwell, J. M., Ellis, J. C. and Levine, J. F. (2014). Seasonal changes in microbial community structure in freshwater stream sediment in a North Carolina River Basin. Diversity, 6: 18-32.
Qu, J., Zhang, Q., Zhang, N., Shen, L. and Liu, P. (2015). Microbial community diversity in water and sediment of a eutrophic lake during harmful algal bloom using MiSeq illumina. technology. International Conference on Advances in Environment Research. 87 (12).
Mueller-Spitz, S. R., Goetz, G. W. and McLellan, S. L. (2009) Seasonal and spatial variability in nearshore bacterioplankton communities of lake Michigan. FEMS Microbiology Ecology, 67: 511–522.
Spain, A. M., Peacock, A. D., Istok, J. D., Elshahed, M. S., Najar, F. Z., Roe, B. A., White, D. C. and Krumholz, L. R. (2007). Identification and isolation of a Castellaniella species important during biostimulation of an acidic nitrate- and uranium-contaminated aquifer. Applied and Environmental Microbiology, 73: 4892-4904.
Burja, A. M., Tamagnini, P., Bustard, M. T. and Wright, P. C. (2001). Identification of the green alga, Chlorella vulgaricus (SDC1) using cyanobacteria derived 16S rDNA primers: targeting the chloroplast. FEMS Microbiology Letters, 202: 195-203.
Udotong, I. R., Uko, M. P. and Udotong, J. I. R. (2018). Prokaryotic Diversity of a Remote Aviation Fuel-Polluted Lentic Ecosystem in Ibeno, Medical and Clinical Research, 3: 1-5.