Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms
International Journal of Animal Science and Technology
Volume 2, Issue 1, March 2018, Pages: 6-13
Received: Nov. 17, 2017; Accepted: Nov. 29, 2017; Published: Jan. 17, 2018
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Kanenus Dereje Dereje, Wama Hagalo Districts of East Wollega Zone, Oromia Region, Nekemte, Ethiopia
Abriham Kebede, School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia
Nigus Abebe, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Yobsan Tamiru, School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia
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A cross-sectional study was conducted from November 2014 to April 2015 to determine the prevalence of bovine mastitis, isolation of mastitis causing bacteria’s and characterize antimicrobial susceptibility pattern at Holeta Agricultural Research Centre (HARC) Dairy Farms. Purposive sampling technique was employed and all lactating cows were involved in the study. Among 186 lactating cows consists of 92 Jersey as well as 94 cross breed (Boran X Holistein Fresian) were included in the study. Among selected cows, 131 (70.43%) cows were identified as bovine mastitis positive and out of which 10 (5.37%) had clinical mastitis and 121 (65.05%) had subclinical mastitis. The different types of bacterial species isolated in present study includes S. aureus (30.93%), staphylococcus other than S. aureus (14.43%), Str. agalactiae (5.15%), Str. Dysgalactiae (5.15%), Str. Uberis (12.37%), C. bovis (13.40%), E. coli (6.18%), P. aerogenosa (10.3%) and K. pneumonie (2.05%). Antimicrobial susceptibility testing revealed that 94% and 78.6% of total isolates were susceptible to gentamycin and trimethoprim-sulfamethoxazole, respectively which was followed by erythromycin and tetracycline with susceptibility rate of 73.8% and 69%, respectively. However, penicillin had shown susceptibility of 55.9% which was the least effective among the drugs used. In conclusion, different types of mastitis causing pathogens with variable rate of susceptibility to antimicrobials were able to identify. Therefore, antimicrobial therapy should be used after isolation of the suspected pathogen to avoid emergence of drug resistance.
Antimicrobial, Dairy, Isolation, Mastitis, Susceptibility, HARC
To cite this article
Kanenus Dereje Dereje, Abriham Kebede, Nigus Abebe, Yobsan Tamiru, Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms, International Journal of Animal Science and Technology. Vol. 2, No. 1, 2018, pp. 6-13. doi: 10.11648/j.ijast.20180201.12
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