Role of Aged Garlic Extract Against Radiation Induced Oxidative Stress Associated with Some Biochemical Disorders in Male Albino Rats
This study was conducted to clarify the potential role of AGE against damages induced in rats due to exposure to gamma radiation. Adult male albino rats (214-230g). Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E+Alpha Lipoïc Acid. Blood samples were collected at day 8 post irradiation for biochemical assay. Exposure of rats to gamma radiation caused a significant increase in the level of total cholesterol (TC), triglycerides (TG), LDL-Cholesterol, Malondialdehyde (MDA), Nitrite (NO2-), Creatinine and AST, ALT, ALP and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin)while a significant decrease was recorded in HDL-Cholesterol, serum total proteins, glutathione content (GSH), superoxide dismutase (SOD), catalase (CAT) activities and total protein level in organs tissues. In rats treated with AGE then exposed to radiation, the results showed an improvement in all previous parameters. It could be concluded that AGE might reduce the biological hazards in rats induced by gamma irradiation.
Kouam Foubi Brice Bertrand,
Chuisseu Djamen Dieudonné Pascal,
Dzeufiet Djomeni Paul Désiré,
Mopia Foubi Myriam Arielle,
Samba Ngano Odette,
Tiedeu Alain Bertin,
Gonsu Fotsin Joseph,
Role of Aged Garlic Extract Against Radiation Induced Oxidative Stress Associated with Some Biochemical Disorders in Male Albino Rats, Journal of Diseases and Medicinal Plants.
Vol. 2, No. 6,
2016, pp. 96-116.
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M and Telser J. Free radicals and antioxidants in normal physiological functions and human disease, The International Journal of Biochemistry & Cell Biology. 2007; 39: 44–84.
Abdel-Magied N and Ahmed A. G. The Protective Role of Lettuce oil (Lactuca sativa) against Radiation induced Biological Hazards in Male Rats. J. Rad. Res. Appl. Sci. 2011; 4(3-4): 923–938.
Sen S, Chakraborty R, Sridhar C, Reddy Y and Biplab De. Free radicals, antioxidants, diseases and phytomedicine: current status and future prospect. International Journal of Pharmaceutical Sciences Review and Research. 2010; 3(1): 91-100.
Kong Ah-Ng T, Yu R, Chen C, Mandlekar S. and Primiano T. Signal transduction events elicited by natural products: Role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 2000; 23: 1–16.
Ahmed R S, Seth V and Banerjee B D. Influence of dietary ginger (Zingiber officinales Rosc) on antioxidant defense system in rat: Comparison with ascorbic acid. Indian J. Exp. Biol. 2000; 38: 604–606.
Naziroglu M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozatocin. 2001; 79: 149–159.
Ramachandran L, Krishnan C, Nair C. Radioprotection by alpha-lipoïc acid palladium complex formulation (POLY-MVA) in mice. Cancer Biotherapy & Radiopharmaceuticals. 2010; 25: 395-399. PMID: 20701542.
Amagase H, Petesch B L, Matsuura H, Kasuga S and Itakura Y. “Intake of garlic and its bioactive components”. Journal of Nutrition, vol. 131, supplements. 2001; 3: 955–962.
Moriguchi T, Saito H, Nishiyama N. Anti-aging effect of aged garlic extract in the inbred brain atrophy mouse model. Clin. Exp. Pharmacol. Physiol. 1997; 24: 235–42.
Balamash K, Albar O, Wang Q and Ahmed N: Effect of Kyolic® aged garlic extract on glycaemia, lipidaemia and oxidative stress in patients with type 2 diabetes mellitus. Journal of Diabetes Research and Clinical Metabolism. 2012; 1: 18.
EEC: Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities1986; L358: 1–29.
Kaplan A. Aspartate aminotransferase. Clin Chem The VC Mosby Co. St Louis. Toronto. Princeton. 1984; p. 1112-116.
Prahlad K V, Conaway C H. Alkaline Phosphatase Activity in the Rat Uterus. J. Embryol. exp. Morph. 1961; 9(4): 599-08.
Gornall A G, Bardawill C J, David M M. Determination of serum proteins by means of biuret reactions. J. Biol,. Chem. 1949; 177: 751-766.
Balistreri W F and Shaw L M. Liver function. In: fundamentals of clinical chemistry. nTeitz, N. W. (ed.). 3rd ed. W. B. Saunders company, Philadelphia. 1987, 729-760.
Atsang A Kiki G. Analgesics and anti-inflammatory properties Dichrostachysglomerata (Huch) (Mimosacée) in rats and mice. PhD Thesis. Animal physiology option. University of Yaounde I. Facebook sciences. 2014, p. 157.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18: 499-502.
Nauck M, Warnick G, Rifai N. Methods for measurement of LDL-cholesterol: a critical assessment of direct measurement by homogeneous assays versus calculation. Clin Chem. 2002, 48(2): 236-54.
Bartels H., Bohmer. Estimation of creatinine clearence in patients with unstable renal function. Clin Chim Acta. 1972; 37: p. 173.
Ellman G L. Tissue sulfhydriyl groups. Arch. Biochem. Biophys. 1959; 82 p. 70-7.
Misra HP, FridovichI. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10): 3170-5.
Sinha KA. Colorimetric essay of catalase. Analyze Biochemistry. 1972; 47: 389-94.
Slack PT. Analytical methods manual, British food Manufacturing industries Research Association, Leatherland, 1987.
Wilbur Km, Bernheim F, Shapiro Ow. The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents. Arch Biochem. 1949; 24(2): 305–313.
Sanaa A Ali, Lila M Fadda, Hesham Elebiary, Miada Soliman. Evaluation of the radioprotective action of anserine along with zinc in albino rats exposed to gamma radiation. Journal of Applied Pharmaceutical Science. 2012; 02(04): 115-122.
Khamis F, Roushdy Hm. Synergistic radio-protective action of imidazole and serotonin on serum and liver enzymes in rats. Arab J Sci App. 1991; 24, 19–36.
O. A. Adaramoye D O. Osaimoje A M, Akinsanya C M, Nneji M A, Fafunso O G, Ademowo. Changes in antioxidant status and biochemical indices after acute administration of artemether, artemether-lumefantrine and halofantrine in rats. J. Compil. Basic Clin. Pharmacol. Toxicol. 2008; 102: 412–418.
Saad, T. M. M. and El Masry, F. S.. Antioxidant activity of lycopene extracted from tomato pomace toward gamma irradiation hazardsin male albino rats. Isotope. Rad. Res. 2005; 37(4): 937-946.
Kumar, M.; Sharma, M.; Saxena, P. and Kumar, A. Radioprotective effect of panaxgensingon the phosphatases and lipid peroxidation level in testis of Swiss albino mice. Biol. Pharm. Bull. 2003; 26(3): 308-312.
Abdel-Fattah, K. I.; El-Sayed, N. M.; Abou-Safi, H. M. and Hussain, A. H. The early alterations in some enzymatic activity, blood glucose and liver glycogen levels induced by atropine injection and whole body irradiation of rats. Egypt J. Rad. Sci. Applic. 1999; 12(2): 37-50.
Moss, D. W.; Henderson, A. R. and Kachmar, J. F. Enzymes In: Fundamentals of clinical chemistry 3rdedn. Tietz, NW Ed. Philadelphia, WB Saunders Company. 1987; 346-421.
El-Kafif, M.; Ragab, M.; El-Dawy, H. and Tawfik, S. Effect of ttaurine treatment on some biochemical parameters in gamma irradiated mice. Environ. Sci.. 2003; 6(2): 393-401.
Albaum, H. C. Serum enzymes following whole body radiation in the rabbit. Rad. Res. 1960; 12: 186-194.
Azab, Kh.; Saada, H. and Said, O. The action of long term treatment with coenzyme Q10 in minimizing radiation-induced damage. Arab. J. Nucl. Sci. Applic. 2001; 34(1): 283-289.
Tabachnick, J.; Perlish, J. S.; Chang, L. F. and Freed, R. M. Enzymatic changes in beta-irradiated epidermis of Guenea pigs: acid and alkaline phosphatase and inorganic pyrophosphatase. Rad. Res. 1967; 32(2): 293-308.
El-Missiry, M. A.; Roushdy, H. M.; Fayed, T. A. and Shehata, G. Role of bone marrow treatment for acute gamma radiationinduced changes in rats. J. Union. Arab. Biol. 1998; 10: 359-370.
Fahim, F.; Roushdy, H.; Yousri, R. and Abady, M. Some biochemical aspects of the protective of strontium chloride on gamma irradiated rats. Biometals. 1993; 6(3): 163-70.
A. S. El-Sharaky, A. A. Newairy, M. A. Kamel, S. M. Eweda, Protectiveeffect of ginger extract against bromobenzene-induced hepatotoxicity in male rats. Food Chem. Toxicol. 2009; 47: 1584–1590.
Mohammed M. M. A. The Possible Protective Role of Foeniculumvulgare Mill. Against Radiation-Induced Certain Biochemical Changes in Albino Rats. Master Degree of Science, Faculty of Science, Beni-Suef University, 2010.
Martin, P. and Freidman, L. S. In: Freidmann, L. S., Kaffee, E. B., ed., Handbook of liver disease. Philadelphia, Churchill Livingstone. 1998; pp 1.
Saraswat, B.; Visen, P. K.; Patnaik, G. K. And Dhawan, B. N. Anticholestatic effect of picroliv, active hepatoprotective principles or princilple of Picrohizakurrooa, against carbon tetra chloride induced cholestasis. Ind. J. Exp. Boil. 1993; 3(4): 316-8.
Omran, M. F.; Abouellela, A. M. K. and Shahian, Y. E. Amifotine (WR2721) drug controls radiation induced damage inrats. Egypt. J. Rad. Sci. Applic. 2009; 22(2): 397-411.
Hassan, S.; Abu-Gahadeer, A. and Osman, S. Vitamins B and/or folic caid restoring the haematopioeic activity in irradiated rats. Egypt. J. Rad. Sci. Applic. 1996; 9(1): 67-78.
V. Padalko, E. Kozlova, I. Leonova, Protective efficacy of garlic on cadmium induced oxidative stress in young and adult rats. Oxid. Antioxid. Med. Sci. 2012; 1(2): 101–109.
A. Sharma, V. Sharma, L. Kansal, Amelioration of lead-induced hepatotoxicity by Allium sativum extracts in Swiss albino mice. Libyan J. Med. 2010; 5: 4621, http://dx.doi.org/10.4176/091107.
H. Alkreathy, A. D. Zoheir, A. Nessar, S. Mark, S. A. Soad, A. M. Osman, Aged garlic extract protects against doxorubicin-induced cardiotoxicity in rats. Food Chem. Toxicol. 2010; 48: 951–956, http://dx.doi.org/10.1016/jfct2010.01.005.
K. Pradeep, C. V. R. Mohan, K. Gobianand, S. Karthikeyan, Silymarin modulates the oxidant–antioxidant imbalance during diethylni-trosamine induced oxidative stress in rats. Eur. J. Pharmacol. 2007; 560: 110–116.
A. S. Nada, A. M. Hawas. Rhubarb extract ameliorates some biochemical disorders induced by gamma irradiation in male rats. J. Rad. Res. Appl. Sci., 2012; 5(2) 393–408.
Horie T, Murayama, T, Mishima T, Fumiyaki I, Minamide Y, Fuwa T, Awaxu S. (1989) Protection of liver microsomal membranes from lipid peroxidation by garlic extract. Planta Med. 1989; 55: 506–8.
Agarwal, K. C. Therapeutic action of garlic constituents. Medical Research Review. 1996; 16: 111-124.
Tadi PP, Teel RW, Lau BHS. Organosulfur compounds of garlic modulate mutagenesis, metabolism and DNA binding of aflatoxin B1. Nutr. Cancer. 1991; 15: 87-95.
Borek C. Molecular mechanisms in cancer induction and prevention. Environ. HealthPerspect. 1993; 101: 237–45.
Borek C. Antioxidants and cancer. Sci. 1997; 4: 51–62.
Harper, H. A.; Rodwell, V. W. and Mayes P. A. (1977): In review of physiological chemistery. 18th ed. Lange Medical Publication. Marzen Company Limited. Los Altos. California. USA. 328.
Maha Mahmoud Ali Mohammed. The Possible Protective Role of FoeniculumvulgareMill. Against Radiation-Induced Certain Biochemical Changes in Albino Rats. Master Degree of Science in Zoology, Faculty of Science, Beni-Suef University, 2010.
M. A. A. Metwally. Effects of Garlic (Allium sativum) on Some Antioxidant Activitiesin Tilapia Nilotica (Oreochromisniloticus). World Journal of Fish and Marine Sciences. 2009; 1(1): 56-64.
Saada, B. N. Studies on the biochemical changes induced by irradiation with special reference to some protecting drug. M. Sc. Thesis, Fac. Of Sci, Ain Sham Univ, 1982.
Roushdy, H. M.; El-Huseini, M. and Saleh, F. Effect of whole body gamma irradiation and/ or dietary protein deficiency on the levels of plasma non-protein nitrogen and amino acids, plasma and urinary ammonia and urea in desert rodent and albino rats. Egypt. J. Rad. Sci. Applic. 1984; 1(2): 157.
Srinivasan, M. N.; Basu, S. K. and Shose, A. Effect of chemical radioprotectors on serum proteins of rats exposed to gamma irradiation. Ind. J. Exp. Biol. 1985; 23(9): 490-2.
Haggag, A. M.; El-Beih, N. M. and Mangood, S. A. In vivo internal decontamination of 134Cs and 60Co from male albino rats. Egypt. J. Rad. Sci. Applic. 2008; 21(2): 531-546.
Reuter, A. M.; Gerber, G. B.; Kennes, F. and Remy-Defraigne, J. Catabolism of serum proteins after x-irradiation. Rad. Res. 1967; 30(4): 725-38.
Takhtayev, T. M. and Tadzh, T. Changes in serum proteins after prolonged irradiation and treatment with leukocytin. Gas. Med. Inst. 1972; 97: 125.
Kafafy, Y. H. and Ashry, O. Antioxidative potential of parsley on gamma irradiated rats. Egypt J. Rad. Sci. Applic. 2001; 14: 25-42.
Roushdy, H. M.; El-Hussaini, M. and Saleh, F. Effect of wholebody gamma-irradiation and/or dietary protein defeciency on levels of plasma non-protein-nitrogen and amino acids in desert rodents and albino rats. Egypt J. Rad. Sci. Applic. 1989; 1: 156-166.
Samarth, R. M.; Goyal, P. K. and Kumar, A. Modulatory effect of Menthapiperitia (Linn.) on serum phophatases activity in Swiss albino mice against gamma-irradiation. Ind. J. Exp. Biol. 2001; 39(5): 479-82.
Kadiiska, M.; Gladen, B.; Baired, D.; Dikolova, A.; Sohal, R.; Hatch, G.; Jones, D.; Mason, R. and Barrett, J. (2000): Biomarkers ofoxidative stress study: are plasma antioxidant markers of CCL4 poisoning? Free. Rad. Biol. Med., 28(6): 838-45.
El-Missiry, M. A.; Fayed, T. A.; El-Sawy, M. R. and El-Sayed, A. A Ameliorative effect of melatonin against gammairradiation induced oxidative stress and tissue injury. Ecotoxicol. Environ. Saf. 2007; 66(2): 278-86.
Ali, M. M.; Noaman, E.; Kamal, Sh.; Soliman, S. and Ismail, D. A. Role of germanium L-cysteine α-tocopherol complex asstimulator of some antioxidant defense system in gamma irradiatedrats. Acta. Pharm. 2007; 57: 1-12.
Bhatia, A. L., Manda, K. Study on pre-treatment of melatonin against radiation-induced oxidative stress in mice. Environ. Toxicol. Pharmacol. 18. 2004; (1): 13-20.
Kempner, E. S. Effects of high-energy electrons and gamma rays directly on protein molecules. J. Pharm. Sci. 2001; 90(10): 1637-1646.
Tanaka T. Cancer chemoprevention by natural products. Oncol. Rep. 1994; 1: 1139-155.
E. Block, “The chemistry of garlic and onions,” Scientific American, 1985; 252(3): 114–119.
G. Hahn, “History, folk medicine, and legendary uses of garlic,” in Garlic: The Science and Therapeutic Application of Allium Sativum L and Related Species, H. P. Koch and L. D. Lawson, Eds., pp. 1–24, Williams & Wilkins, Baltimore, Md, USA, 1996.
Ramadan F. Induced Defects in Pregnant Rats and Their fetuses Treated with Fluconazole and/or Gamma Radiation. Arab J. Nucl. Sci. Appl. 2013; 46(1): 282-96.
Y. Fang, S. Yang, and G. Wu. Free radicals, antioxidants, and nutrition. Nutrition. 2002; 18(10): 872-9.
Motoyama T, Okamoto K, Kukita I,. Hamaguchi M, Kinoshita Y, Ogawa H. Possible Increased role of oxidant stress in multiple organ failure after systemic inflammatory response syndrome. Crit. Care Med. 200; 31(4): 1048-52.
Dahlen G, Guyton J. R, Arrar M, Farmer Ja. et al. Association of levels of lipoprotein (a), plasma lipids, and other lipoproteins with coronary artery disease documented by angiography. Circulation. 1986; 74: 758–65.
Havel Rj, Kane Jp. Structure and metabolism of plasma lipoproteins. In: CR Scriver, AL Beaudet, WS Sly and D Valle, eds. The metabolic and molecular basis of inherited disease, 7th edition. McGraw- Hill, USA. 1995; 1841-1851.
Bass K. M, Newschaffer CJ, Klag, MJ, Bush TL. Plasma lipoprotein levels as predictors of cardiovascular death in women. Arch Intern Med. 1993; 153: 2209.
Nada, A. S. Modulating efficacy of rosemary extracts in rats exposed to oxidative stress. Egypt. J. Rad. Sci. Applic. 2008; 21(2): 499-514.
Kafafy, Y. A.; Roushdy, H.; Abdel-Haliem, M.; Mossad, M.; Ashry, O. and Salam, S. Green tea antioxidative potential in irradiated pregnant rats. Egypt. J. Rad. Sci. Applic. 2005b, 18(2): 313-333.
Zahran, A. M.; Noaman, E.; Omran, M. F. and Said, K. Influence of some micronutrients quenshing the effect of gamma radiation on plasma lipids and vitamin E contents in rats. Egypt. J. Rad. Sci. Applic. 2003; 16(1): 71-84.
Markevich, L. N. and Kolomiitseva, I. K. Lipids from liver microsomes and mitochonderia upon acute and chronic gamma irradiation of rats. Biokhimiia Jull. 1994; 59(7): 102733.
Said, U. Z. and Azab, Kh. Sh. Efficacy of wheat germ oil in modulating radiation induced heat damage. Egypt. J. Rad. Sci. Applic. 2006; 19(2): 433-452.
Roushdy, H. M.; Abdel-Hamid, F. M. and Abu-Ghadeer, A. R. Impairment of liver and kidney functions in gamma irradiation rats suffering from pesticide toxicity. Isotope Rad., Res. 1997; 29(1-2): 11-21.
Bok, S.; Lee, S.; Park, Y.; Bae, K.; Son, K.; Jeong, T. and Choi, M. Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-CoA reductase and aryl CoA: Cholesterol transferase is lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids. J. Nutr. 1999; 129(6): 1182–1185.
Abd El-Gawad, E. I. and Aiad, S. K. Antioxidant and hypolipidemic effect of raw green snap (Phaseolus vulgaris) on aged male rats exposed to γ-radiation. Egypt. J. Rad. Sci. Applic. 2008; 21(1): 131-144.
Sedlakova, A.; Borlkova, K. and Ahlers, I. Canges in lipoprotein lipase activity in the adipose tissue and heart of non-lethalyxirradiated rats. Physiol. Bohemoslov. 1986; 35(5): 400-5.
Mahmoud, K. Response of certain biochemical parameters to whole body gamma irradiation in albino rats and synergestic effect of pharmacological agent. Ph. D. Thesis, Fac. Sci. Alex. Univ; 1996.
Feurgard, C.; Boehler, N.; Férézou, J.; Sérougne, C.; Aigueperse, J.; Gourmelon, P.; Lutton, C.; and Mathé, D. Ionizingradiation alters hepatic cholesterol metabolism and plasma lipoproteins in Syrian hamaster. Int. J. Rad. Biol. 1999; 75(6): 757-766.
Chaialo, P. P.; Liakhovchnk, N. N. and Chovot′ko, G. M. Effect of a sigle and fractionated gamma-irradiation on lipid content in rat blood and tissues. Ukr. BioKhim. Zh. 1992; 64(5): 60-66.
Clarke, S. D. Poly unsaturated fatty acid regulation of gene transcription: A molecular mechanism to improve the metabolic syndrome. J. Nutr. 2001; 131(4): 1129-1132.
Feurgard, C.; Bayle, D.; Guézingar, F.; Sérougne, C.; Mazur, A.; Lutton, C.; Aigueperse, J.; Gourmelon, P. and Mathé, D. Effects of ionizing radiation (neutron/gamma rays) on plasma lipids and lipoproteins in rats. Rad. Res. 1998; 150(1): 43- 51.
Baker, J. E.; Fisk, B. L.; Su, J.; Haworth, S. T.; Strande, J. L.; Komorowski, R. A.; Migrino, R. Q.; Doppalapudi, A.; Harmann, L.; Allen Li, X.; Hopewell, J. W. and Moulder, J. E. 10 Gy total body irradiation increases risk of coronary sclerosis, degeneration of heart structure and function in a rat model. Int. J. Radiat. Biol. 2009; 85(12): 1089-1100.
Chajek-Shaul, T.; Friedman, G.; Stein, O.; Shiloni, E.; Etienne, J. and Stein, Y. Mechanism of the hypertriglyceridemia induced by tumor necrosis factor administration to rats. Biochem. Biophys. Acta. 1989; 1001(3): 316-324.
Madamanchi, N. R. and Runge, M. S. Mitochondrial dysfunction in atherosclerosis. Circul. Res. 2007; 100(4): 460-473.
Chrysohoou, Ch.; Panagiotakos, D.; Pitsavos, Ch.; Kosma, K.; Barbetseas, J.; Karagiorga, M.; Ladis, I. and Stefanadis, Ch. Distribution of serum lipids and lipoproteins in patients with beta thalessaemia major; an epidemiological study in young adults from Greece. Lipids in Health Dis. 2004; 3: 3.
Ahmed, G. A. The prospective role of natural product application in diminished some physiological disorders caused by gamma radiation in rats. Ph. D. Thesis Faculty of Science Ain shams University Egypt; 2006.
Abdel-Magied, N., Experimental studies on the protective role of onion oil (Allium cepa Linn) against radiation induced biological hazards in mammalian tissues. Ph. D. thesis, Faculty of Science, Tanta Univ., Egypt; 2007.
Sedlakova, A; Ahlers, I.; Ahlersova, E.; Malatova, Z.; Paulikova, E. and praslicka, M. The dynamics of changes in serum lipids during continuous irradiation of rats. Folia. Biol. (Praha). 1977; 23(4): 291-298.
Khalid S. Al-Numair. Hypocholesteremic and Antioxidant Effects of Garlic (Allium sativum L.) Extract in Rats Fed High Cholesterol Diet. Pakistan Journal of Nutrition. 2009; 8(2): 161-166.
Bordia, A. K., S. K. Sodhya, A. S. Rathore and N. Bhu,. Essential oil of garlic on blood lipids and fibrinolyitc activity in patients of coronary artey disease. J. Asso. Physicians Ind. 1975; 26: 327-331.
Jain, R. C.. Effect of garlic on serum lipids, coagulability and fibrinolytic activity of blood. Am. J. Clin. Nutr. 1977; 30: 1380-1381.
Kumar, G. R. and K. P. Reddy. Reduced nociceptive responses in mice with alloxan induced hyperglycemia after garlic (Allium sativum Linn.) treatment. Indian J. Exper. Biol. 1999; 37: 662-666.
Thomson, M. and M. Ali,. Garlic (Allium sativum): A review of its potential use as an anti- cancer agent. Current Cancer Drug Targets. 2003; 3: 67-81.
Khaki A., Fathiazad F., Nouri M., Khaki A. A., Khameneki H. J., Hamadeh M. Evaluation of androgenic activity of Allium cepaon spermatogenesis in the rat. Folia Morphology. 2009; 68(1): 45–51.
Augusti, K. T.. Hypocholesterolemic effect of garlic, Allium sativum Linn. Ind. J. Exp. Biol. 1977; 15: 489-490.
Sodimu, O., P. K. Joseph and K. T. Augusti,. Certain biochemical effects of garlic oil on rats maintained on high fat- high cholesterol diet. Experiential. 1984; 40: 78-80.
Banerjee. S. K., M. Maulik, S. C. Mancahando, A. K. Dinda, S. K. Gupta and S. K. Maulik. Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic. Life Sci. 2002; 70: 1509-1518.
Ou, C. C., S. M. Tsao, M. C. Lin and M. C. Yin,. Protective action on human LDL against oxidation and glycation by four organosulfur compounds derived from garlic. Lipids. 2003; 38: 219-224.
Thomson, M., K. K. Al-Qattan, T. Bordia and M. Ali, Including garlic in the diet may help lower blood glucose, cholesterol and triglycerides. J. Nutr. 2006; 136: 800-802.
Gebhardt, R.. Inhibition of cholesterol biosynthesis by water soluble garlic extract in primary cultures of rat hepatocytes. Arzneimittelforschung. 1991; 41: 800-804.
Gebhardt, R. and H. Beck. Differential inhibitory effects of garlic-derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids. 1996; 1269-1276.
Durak, L., M. Kavutcu, B. Aytac, A. Avci, E. Devrim, H. Özbek and S. H. Öxtürk,. Effect of garlic extract consumption on blood lipid and oxidant/antioxidant parameters in humans with high blood cholesterol. J. Nutr. Biochem., 2004; 15: 373-377.
Ramadan, L.; Ghazly, M. A.; Moussa, L.; Saleh, S.; El-Denshary, E. and Roushdy, H. Radioprotective effect of thiola on radiation induced functional and structural changes in rat intestine. Egypt. J. Rad. Sci. Applic. 1998; 10(11): 39.
Altman, K. I.; Gerber, G. B. and Okada, S. In ''Radiation Biochemistery ''Tissues and body fluids. Acad. Press. N. Y. and London. 1970; 2: 260.
Roushdy, H. M.; El-Husseine, M. and Saleh, F. Response of plasma and urinary uric acid, creatine and creatinine to dietry protein deficiency and or whole body γ-radiation in desert rodent and albino rats. Egypt. J. Rad. Sci. Applic. 1985; 2(2): 113.
Hassan, S.; Abu-Ghadeer, A. R.; Osman, S. A. and Roushdy, H. M. Possible curative role of antisycholic drug ''Fluphenazine" against post-irradiation injury in rats. Egypt. J. Rad. Applic. 1994; 7(2): 181.
El-Kashef, H. S. and Saada, H. N. Changes in the level of urea, carotene and creatinine in liver and serum of irradiated rats. Isotope. Rad. Res. 1988; 20(1): 43-50.
Konnova, L.; Konnov, B. and Komar, V. Indices of protein and amino acid metabolism at early periods following whole body uniform gamma and proton irradiation. Radiobiol. 1991; 31(3): 410-413.
Yildiz, F.; Atahan, I. L.; Tuncel, M. and Konan, A. The influence of dose per fraction on the pathogenesis of radiation nephropathy. Australas. Radiol. 1998; 42(4): 347-353.
562 Robbins, M.; O’Malley, Y.; Davis, C. and Bonsib, S. The role of tubulointerstitium in radiation-induced renal fibrosis. Rad. Res. 2001, 155: 481-489.
Jamshid Ghiasi Ghalehkandi1, Yahya Ebrahimnezhad1, Ramin Salamatdout Nobar1. Effect of Garlic (Allium sativum) Aqueous Extract on serum values of Urea, Uric-Acid and Creatinine compared with Chromium Chloride in Male Rats. Annals of Biological Research. 2012; 3(9): 4485-4490.
Li, L. End-stage renal disease in China. Kidney Int. 1996; 49: 287-301.
Bhandari PR. Garlic (Allium sativum L.): A review of potential therapeutic applications. Int J Green Pharm. 2012; 6: 118-29.
Cruz C, Correa-Rotter R, Sánchez-González DJ, Hernández-Pando R, Maldonado PD, Martínez-Martínez CM, Medina-Campos ON, Tapia E, Aguilar D, Chirino YI, Pedraza-Chaverri J. Renoprotective and antihypertensive effects of S-allylcysteine in 5/6 nephrectomized rats. Am J Physiol Renal Physiol. 2007; 293: 1691-8.
Spitz, D. R.; Azzam, E. I.; Li, J. J. and Guis, D. Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: a unifying concept in stress response biology. Cancer and Metastasis Rev. 2004; 23(3-4): 311-322.
Joshi, R.; Kamat, J. P. and Mukherjee, T. Free radical scavenging reactions antioxidant activity of embelin: Biochemical and pulse radiolytic studies. Chem. Biol. Interact.. 2007; 167(2): 125-34.
Millar, T. M. Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthene oxidase. FEBS Lett., 2004; 562(1-3): 129-133.
Koc, M.; Taysi, S.; Buyukokuroglu, M. E. and Bakan, N. Melatonin protects rat liver against irradiation-induced oxidative injury. J. Rad. Res. 2003; 44(3): 211-5.
Samarth, R. M. and Kumar, A. Radioprotection of Swiss albino mice by plant extract Menthapiperita (Linn). J. Rad. Res. 2003; 44(2): 101-9.
Bhatia, A. L. and Jain, M. Spinaciaolleracea L. protectects against gamma radiations, a study on glutathione and lipid peroxidation in mouse liver. Phytomedicine. 2004; 11(7-8): 607-15.
Siems, W.; Gartner, C.; Kranz, D.; Schneider, T.; Grune, T.; Schimke, J.; Gau, S.; Wege, U. and Gerber, G. Long term effects of monthly low dose whole body gamma irradiation on the glutathione status and thiobarbituric acid-reactive substances in different organs of male wistar rats. Radiobiol. Radiother. (Berl.). 1990; 31(3): 257-236.
El-Habit, O. H.; Saada, H. N.; Azab, K. S.; Abdel-Rahman, M. and El-Malah, D. F. The modifying effect of beta-carotene on gamma radiation-induced elevation of oxidative reactions and genotoxicity in male rats. Mudat. Res. 2000; 466(2): 179.
Yanardag, R.; Blokent, S. and Kizer, A. Protective effects of DL-α-tocopherol acetate and sodium selenate on the liver of rats exposed to gamma radiation. Biol. Trace. Elem. Res. 2001; 83(3): 263-273.
Ueda, T.; Toyoshima, Y.; Kushihashi, T.; Hishida, T. and Yasuhara, H. Effect of dimethylesulphoxide pretreatment on activities of lipid peroxidation formation, superoxide dismutase and glutathione peroxidase in the mouse liver after whole-body irradiation. J. Toxicol. Sci.. 1993; 18(4): 239-44.
Haas, M.; Kerjaschki, D. and Mayer, G. Lipid lowering therapy in memberaneous nephropathy. Kidney. Internat. Suppl. 1999; 56: 110-112.
Ramadan, L. A.; Shouman, S. A.; Sayed-Ahmed M. M. and El-Habit, O. H. Modulation of radiation-induced organs toxicity by cremophorel in experimental animals. Pharmacol. Res. 2001; 43(2): 185-91.
Pulpanova, J.; Kovarova, H. and Ledvina, M. Change in the 0reduced Glutathione concentration and the activity of glutathione reductase in some tissues of the rats gamma-irradiated after administration of cystamine. Radiobiol. Radiother. 1982; 23(1): 50-6.
Osman, S. A. A. Melatonin beosting antioxidant capacity for effective radiation protection in albino rat. Egypt. J. Med. Sci.. 1996; 17(2): 281-295.
El-Ghazaly, M. A. and Ramadan, L. A. Alterations in some metabolic functions in albino rats after irradiation and possible role of thiola. J. Egypt. Soc. Toxicol. 1996; 16: 105-9.
Rajapakse, N.; Kim, M. M.; Mendis, E. and Kim, S. K. Inhibition of free radical-mediated oxidation of cellular biomolecules by carboxylated chitooligosaccharides. Bio-org. Med. Chem. 2007; 15(2): 997-1003.
Saada, H. A.; Azab, Kh. Sh.; Said, O. Z.; Mohamed, M. A. and Abbady, M. M. Role of genesing in the control of radiation induced injury in rat erythrocyte. J. Egypt. Ger. Soc. Zool. 1999; 28(A): 191-9.
Abu-Ghadeer, A. R. M.; Osman, S. A. and Abbady, M. M. Garlic protects the glutathione redox cycle in irradiated rats. Egypt. J. Rad. Sci. Applic. 1999; 12(2): 15-26.
Estuo N.. Lipid peroxidation: physiological levels and dual biological effects, Free Radic. Biol. Med. 2009; 47(5) 469–484.
Halliwell, B. and Gutterige, J. Protection against oxidants in biological systems; the superoxide theory of oxygen toxicity. In: Free Radicals in Biology and Medicine 2nd ed., 86. Kindersley, London; 1989:.
Siems, WG.; Grune, T. and Esterbauer H. 4-hydroxynonenal formation during ischemia and reperfusion of rat small-intestine, Life Science. 1995; 57, 785–789.
Rotruck, J. T.; Pope, A. L.; Ganther, H.; Awanson, A. B.; Hoffeman, D. G. and Hofckstra, W. G. Selenium: Biochemical role as a component of glutathione peroxides. Sci. 1979; 179: 588-590.
Dahm, L. J.; Bailie, M. B. and Roth, R. A. Relationship between alpha-naphthylisothiocyanate-induced liver injury and elevations in hepatic non-protein sulfhydryl content. Biochem. Pharmacol. 1991; 42(6): 1189-94.
Dharmendra K Maura, Thomas P A Devasagayam and Cherpall Krishnan K Nair. Some novel approaches for radioprotection and the beneficial effect of natural products. Indian journal of experimental Biolog Vol. 44, February. 2006; 93-114.
Zhao Y, Shen S, Ling B. Protection by EGCG of DNA from radiation damage Chayekexue. 1995; 15: 145-48.
Piya Paul, M K Unnikrishnan and A N Nagappa. Phytochemicals as radioprotective agents-A Review. Indian Journal of Natural Products and Resources. 2011; 2(2): 137-150.
Ana L. C., Santana R. A., Silva-Islas C. A., Chanez-Cardenas M. E., Santamarıa A., Maldonado P. D. The antioxidant mechanisms underlying the aged garlic extract- and s-allyl cysteine-induced protection, Oxid. Med. Cell. Longev. (2012) 16, http://dx.doi.org/10.1155/2012/907162, Article ID 907162.
Capasso A., Antioxidant action and therapeutic efficacy of Allium sativum L., Molecules 18. 2013; 1: 690–700, http://dx.doi.org/10.3390/molecules18010690.
Almaca G., Antioxidant effects of sulfur containing amino acids, Yon-seiMed. J. 2004; 45(5): 776–788.
Carmia Borek. Antioxidant Health Effects of Aged Garlic Extract. J. Nutr. 2001; 131(3) 1010-1015.
Arivazhagan, S., S. Balascnthil and S. Nagini, 2000. Friedewald, W. T., R. I. Levy and D. S. Fredrickson, Garlic and neem leaf extracts enhance hepatic glutathione and glutationic-dependent enzymes during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in rats. Phytotherapy Res. 1972; 14: 291-293.
Arivazhagan, S., S. Balascnthil and S. Nagini,. Chemopreventive potential of garlic and neem during gastric carcionogensis induced by N-methyl-N'-nitro-N-nitrosoguanidine. Med. Sci. Res. 1999; 27: 209-212.
Gorinstein S., Leontowicz H., Leontowicz M., Drzewiecki J., Naj-man K., Katrich E., Barasch D., Yamamoto K., Trakhtenberg S. Raw and boiled garlic enhances plasma antioxidant activity and improves plasma lipid metabolism in cholesterol-fed rats, Life Sci. 2006; 78: 655–663.
Arivazhagan, S., S. Balascnthil and S. Nagini,. Modulatory effects of garlic and neem leaf extracts on circulatory lipid peroxides and antioxidants during N-methyl-N'-nitro-N-nitrosogunidine-induced gastric carcinogensis. Med. Sci. Res. 1999; 27: 527-529.
Balascnthil, S., S. Arivazhagan, C. R. Ramachandran and S. Nagini,. Effects of garlic on 7, 12-dimethylbenz [a] anthracene-induced hamster buccal pouch carcinogenesis. Cancer Detection and Prevention. 1999; 23: 534-538.
Ashraf Y. Nasr. Protective effect of aged garlic extract against the oxidative stress induced by cisplatin on blood cells parameters and hepatic antioxidant enzymes in rats. Toxicology Reports. 2014; 1: 682–691.
Wei Z. Lau B. Garlic inhibits free radical generation and augments antioxidant enzyme activity in vascular endothelial cells. Nutr. Res. 1998; 18: p. 61–70.
Pinto, J. T., C. Qiao and J. Zing,. Effects of garlic. thioallyl derivatives on growth, glutathione concentration and polyamine formation of human prostate carcinoma cells in culture. Am. J. Clin. Nutr. 1997; 66: 398-405.
Prasad, K., V. A. Laxdal, M. Yu and B. L. Raney, Antioxidant activity of allicin and active principal in garlic. Mol. Cell. Biochem. 1995; 148: 183-189.
Liu J, Lin X, Milner J. Dietary garlic powder increases glutathione content and glutathione S-transferase activity in rat liver and mammary tissues. FASEB J. 1992; 6: 3230.
Lau BHS. Detoxifying, radioprotective and phagocyte-enhancing effects of garlic. Int. Nutr. 1989; 9: p. 27-31.
Geng Z, Lau BHS. Aged garlic extracts modulate glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells. Phytother. Res. 1999; 11: p. 54-6.