Journal of Diseases and Medicinal Plants

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Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria

Received: 25 November 2018    Accepted: 17 December 2018    Published: 22 January 2019
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Abstract

The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.

DOI 10.11648/j.jdmp.20180406.11
Published in Journal of Diseases and Medicinal Plants (Volume 4, Issue 6, December 2018)
Page(s) 133-141
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Punica granatum Peels, Antibacterial Activity, Antibiofilm Activity

References
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Author Information
  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon; Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, Beirut, Lebanon; Department of Microbiology and Parasitology, University of Navarra, Pamplona, Spain

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon; Center for Research and Analysis (CAR), Research Unit of Agro-Food Technologies and Valorization, Faculty of Sciences, Saint-Joseph University, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon; Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, Beirut, Lebanon

Cite This Article
  • APA Style

    Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. (2019). Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. Journal of Diseases and Medicinal Plants, 4(6), 133-141. https://doi.org/10.11648/j.jdmp.20180406.11

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    ACS Style

    Salam Nasreddine; Amale Mcheik; Mohammad Abdalla Al-Seblani; Hawraa Shahrour; Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J. Dis. Med. Plants 2019, 4(6), 133-141. doi: 10.11648/j.jdmp.20180406.11

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    AMA Style

    Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J Dis Med Plants. 2019;4(6):133-141. doi: 10.11648/j.jdmp.20180406.11

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  • @article{10.11648/j.jdmp.20180406.11,
      author = {Salam Nasreddine and Amale Mcheik and Mohammad Abdalla Al-Seblani and Hawraa Shahrour and Mariam Hammoud and Ali Chokr},
      title = {Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {4},
      number = {6},
      pages = {133-141},
      doi = {10.11648/j.jdmp.20180406.11},
      url = {https://doi.org/10.11648/j.jdmp.20180406.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jdmp.20180406.11},
      abstract = {The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria
    AU  - Salam Nasreddine
    AU  - Amale Mcheik
    AU  - Mohammad Abdalla Al-Seblani
    AU  - Hawraa Shahrour
    AU  - Mariam Hammoud
    AU  - Ali Chokr
    Y1  - 2019/01/22
    PY  - 2019
    N1  - https://doi.org/10.11648/j.jdmp.20180406.11
    DO  - 10.11648/j.jdmp.20180406.11
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 133
    EP  - 141
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20180406.11
    AB  - The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.
    VL  - 4
    IS  - 6
    ER  - 

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