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Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi

Paul Malaba Makoye, Innocent John Daniel, Mourice Nyangabo Mbunde, Nelson Enos Masota, Joseph Sempombe, Veronica Mugoyela
Received: 23 July 2020    Accepted: 4 August 2020    Published: 18 August 2020
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Abstract

This study investigated the antibacterial activities of crude extracts of three Tanzanian plants; Sorindeia madagascariensis, Mucuna stans and Albizia harveyi, following reports on their ethnomedicinal applications and those of their related species. The reported ethnomedicinal applications of the selected plants include treatment of; tuberculosis, urinary tract infections and bacterial infections of the skin among other applications. Plant material were collected from Njombe, Iringa and Pwani regions of Tanzania. Phytochemical screening and bioautography were conducted as per adopted methods. Screening for antibacterial activity was done by broth microdilution assay against the standard and clinical isolates of bacteria. Phytochemical screening revealed the presence of phenolics, tannins, flavonoids, terpenoids and glycosides among the plant extracts. Antibacterial activity-study displayed weak to moderate antibacterial activities of the plant extracts, whereby S. madagascariensis leaf extract displayed the highest activity against; Staphylococcus aureus (ATCC 25923), clinical isolate of S. aureus and a methicillin-resistant S. aureus (MRSA) isolate, at a minimum inhibitory concentration (MIC) of 192±0.00 µg/mL. Bioautography of S. madagascariensis indicated this antibacterial activity to be associated with polar compounds. MICs observed due to M. stans ranged from 770 to 3080 µg/mL against all tested bacterial species whereas the observed MICs due to A. harveyi ranged from 1283 to > 3080 µg/mL. These findings reveal the antibacterial activities of the selected plants, corroborating their ethnomedicinal applications. Bioautography-guided isolation of compounds from these plants particularly S. madagascariensis, may give leads for newer antibacterial agents.

DOI 10.11648/j.jdmp.20200603.12
Published in Journal of Diseases and Medicinal Plants (Volume 6, Issue 3, September 2020)
Page(s) 65-71
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Keywords

Phytochemical Screening, Antibacterial Activity, Bioautography, S. madagascariensis, M. stans, A. harveyi

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    Paul Malaba Makoye, Innocent John Daniel, Mourice Nyangabo Mbunde, Nelson Enos Masota, Joseph Sempombe, et al. (2020). Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi. Journal of Diseases and Medicinal Plants, 6(3), 65-71. https://doi.org/10.11648/j.jdmp.20200603.12

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    ACS Style

    Paul Malaba Makoye; Innocent John Daniel; Mourice Nyangabo Mbunde; Nelson Enos Masota; Joseph Sempombe, et al. Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi. J. Dis. Med. Plants 2020, 6(3), 65-71. doi: 10.11648/j.jdmp.20200603.12

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    AMA Style

    Paul Malaba Makoye, Innocent John Daniel, Mourice Nyangabo Mbunde, Nelson Enos Masota, Joseph Sempombe, et al. Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi. J Dis Med Plants. 2020;6(3):65-71. doi: 10.11648/j.jdmp.20200603.12

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  • @article{10.11648/j.jdmp.20200603.12,
  author = {Paul Malaba Makoye and Innocent John Daniel and Mourice Nyangabo Mbunde and Nelson Enos Masota and Joseph Sempombe and Veronica Mugoyela},
  title = {Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {6},
  number = {3},
  pages = {65-71},
  doi = {10.11648/j.jdmp.20200603.12},
  url = {https://doi.org/10.11648/j.jdmp.20200603.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20200603.12},
  abstract = {This study investigated the antibacterial activities of crude extracts of three Tanzanian plants; Sorindeia madagascariensis, Mucuna stans and Albizia harveyi, following reports on their ethnomedicinal applications and those of their related species. The reported ethnomedicinal applications of the selected plants include treatment of; tuberculosis, urinary tract infections and bacterial infections of the skin among other applications. Plant material were collected from Njombe, Iringa and Pwani regions of Tanzania. Phytochemical screening and bioautography were conducted as per adopted methods. Screening for antibacterial activity was done by broth microdilution assay against the standard and clinical isolates of bacteria. Phytochemical screening revealed the presence of phenolics, tannins, flavonoids, terpenoids and glycosides among the plant extracts. Antibacterial activity-study displayed weak to moderate antibacterial activities of the plant extracts, whereby S. madagascariensis leaf extract displayed the highest activity against; Staphylococcus aureus (ATCC 25923), clinical isolate of S. aureus and a methicillin-resistant S. aureus (MRSA) isolate, at a minimum inhibitory concentration (MIC) of 192±0.00 µg/mL. Bioautography of S. madagascariensis indicated this antibacterial activity to be associated with polar compounds. MICs observed due to M. stans ranged from 770 to 3080 µg/mL against all tested bacterial species whereas the observed MICs due to A. harveyi ranged from 1283 to > 3080 µg/mL. These findings reveal the antibacterial activities of the selected plants, corroborating their ethnomedicinal applications. Bioautography-guided isolation of compounds from these plants particularly S. madagascariensis, may give leads for newer antibacterial agents.},
 year = {2020}
}

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  • TY  - JOUR
T1  - Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi
AU  - Paul Malaba Makoye
AU  - Innocent John Daniel
AU  - Mourice Nyangabo Mbunde
AU  - Nelson Enos Masota
AU  - Joseph Sempombe
AU  - Veronica Mugoyela
Y1  - 2020/08/18
PY  - 2020
N1  - https://doi.org/10.11648/j.jdmp.20200603.12
DO  - 10.11648/j.jdmp.20200603.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 65
EP  - 71
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20200603.12
AB  - This study investigated the antibacterial activities of crude extracts of three Tanzanian plants; Sorindeia madagascariensis, Mucuna stans and Albizia harveyi, following reports on their ethnomedicinal applications and those of their related species. The reported ethnomedicinal applications of the selected plants include treatment of; tuberculosis, urinary tract infections and bacterial infections of the skin among other applications. Plant material were collected from Njombe, Iringa and Pwani regions of Tanzania. Phytochemical screening and bioautography were conducted as per adopted methods. Screening for antibacterial activity was done by broth microdilution assay against the standard and clinical isolates of bacteria. Phytochemical screening revealed the presence of phenolics, tannins, flavonoids, terpenoids and glycosides among the plant extracts. Antibacterial activity-study displayed weak to moderate antibacterial activities of the plant extracts, whereby S. madagascariensis leaf extract displayed the highest activity against; Staphylococcus aureus (ATCC 25923), clinical isolate of S. aureus and a methicillin-resistant S. aureus (MRSA) isolate, at a minimum inhibitory concentration (MIC) of 192±0.00 µg/mL. Bioautography of S. madagascariensis indicated this antibacterial activity to be associated with polar compounds. MICs observed due to M. stans ranged from 770 to 3080 µg/mL against all tested bacterial species whereas the observed MICs due to A. harveyi ranged from 1283 to > 3080 µg/mL. These findings reveal the antibacterial activities of the selected plants, corroborating their ethnomedicinal applications. Bioautography-guided isolation of compounds from these plants particularly S. madagascariensis, may give leads for newer antibacterial agents.
VL  - 6
IS  - 3
ER  -

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Author Information

  • Paul Malaba Makoye

    Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

  • Innocent John Daniel

    Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

  • Mourice Nyangabo Mbunde

    Department of Natural Products Development and Formulation, Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

  • Nelson Enos Masota

    Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania; Institute for Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany

  • Joseph Sempombe

    Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

  • Veronica Mugoyela

    Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

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