Determining the Efficiency of Malaria Rapid Diagnostic Test to Diagnose Post Transfusion Malaria in Children Under Five Years Old at Komfo Anokye Teaching Hospital
Central African Journal of Public Health
Volume 6, Issue 3, June 2020, Pages: 122-130
Received: Feb. 19, 2020; Accepted: Mar. 3, 2020; Published: Mar. 17, 2020
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Djeneba Bouare, School of Medicine and Health Sciences, University for Developement Studies, Tamale, Ghana
Owusu Ofori Alex, Kumasi Centre for Collaborative Research (KCCR), Kumasi, Ghana
Mohammed Abdul-Rahim, Kumasi Centre for Collaborative Research (KCCR), Kumasi, Ghana
Der Muonir Edmund, Department of Pathology, School of Medicine and Health Sciences, University for Development Studies, Tamale, Ghana
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Background: Post-transfusion malaria in children under five years is associated with increased morbidity and mortality. The RDTs currently are used to supplement microscopy in the diagnosis of malaria in many endemic countries. However their efficiency as a screening tool for the detection of malaria in donors or in transfusion recipients has not been well evaluated. This study aimed to determine the efficiency of malaria RDT in the detection of post transfusion malaria. Material and methods: The study was conducted from November 2014 to April 2015. Blood samples from 179 patients and their corresponding donors were tested for Plasmodium parasitaemia using RDT and microscopy. The recipients’ blood samples were obtained within 48 hours after transfusion for malaria diagnosis. To determine the incidence of post transfusion malaria, 32 patients were purposefully selected. Their pre-transfusion, post transfusion and donor blood samples were tested for malaria using PCR. Results: Approximately 52% of the study population were within the age group of 0 - < 1 year. Prevalence of malaria in blood donors was 27% by RDT and 7% by microscopy. Prevalence at pre-transfusion was 34% by RDT and 13% by microscopy. Prevalence at post transfusion was 59% by RDT and 4% by microscopy. Incidence of post transfusion malaria by PCR was 57%. The sensitivity of RDT in detecting malaria at post transfusion was 100%. Conclusion: Efficiency of malaria RDT as a tool for malaria diagnosis at post transfusion, among children under five years old is good. Clinicians should consider implementing the use of malaria RDT at post transfusion.
Under-Five, Blood Transfusion-Associated Malaria, Post-Transfusion, RDT, PCR, Kumasi
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Djeneba Bouare, Owusu Ofori Alex, Mohammed Abdul-Rahim, Der Muonir Edmund, Determining the Efficiency of Malaria Rapid Diagnostic Test to Diagnose Post Transfusion Malaria in Children Under Five Years Old at Komfo Anokye Teaching Hospital, Central African Journal of Public Health. Vol. 6, No. 3, 2020, pp. 122-130. doi: 10.11648/j.cajph.20200603.13
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Elphinstone, R. E., Higgins, S. J., & Kain, K. C. Prevention of Malaria in Travelers: Bite Avoidance and Chemoprophylactic Measures. Current Treatment Options in Infectious Diseases, 2014; 6: 47-57.
World Health Organization (WHO). World malaria report 2014. From Accessed on July 2015.
Nyarko, S. H., Cobblah, A. Sociodemographic Determinants of Malaria among Under-Five Children in Ghana. Malaria research and treatment, 2014.
Buabeng, K. O., Duwiejua, M., Dodoo, A. N., Matowe, L. K., Enlund, H. Self-reported use of anti-malarial drugs and health facility management of malaria in Ghana. Malaria journal, 2007; 6: 85.
Owusu-Ofori, A. Transfusion-transmitted malaria and bacterial infections in a malaria endemic region. (2012; Doctoral dissertation, University of Liverpool.
O’Brien et al, 2015 O’Brien, S. F., Delage, G., Seed, C. R., Pillonel, J., Fabra, C. C., Davison, K & Leiby, D. A. The Epidemiology of Imported Malaria and Transfusion Policy in 5 Nonendemic Countries. Transfusion medicine reviews. 2015.
Austin N, Adikaibe E, Ethelbert O, Chioma U, Ekene N. Prevalence and Severity of Malaria Parasitemie among Children Requiring Emergency, Blood transfusion in tertiary hospital in Imo state, Nigeria. Annals of Medical and Health Science Research. 2014; 4: 19-23.
Erhabor Osaro, Adias Teddy Charles. The challenges of meeting the blood transfusion requirements in Sub-Saharan Africa: the need for the development of alternatives to allogenic blood. Journal of Blood Medicine, 2011; 2: 7–21.
Mark E. Brecher and Shauna N. Hay. Bacterial Contamination of Blood Components Department of Pathology and Laboratory Medicine, University of North Carolina. Journal of Clinical Microbiololy Review, 2005; 18: 195–204.
Jobert Richie N Nansseu, Jean Jacques N Noubiap, Shalom Tchokfe Ndoula, Albert Frank M Zeh and Chavely Gwladys Monamele. (2013). What is the best strategy for the prevention of transfusion-transmitted malaria in sub-Saharan African countries where malaria in endemic? Malaria Journal, 2013; 12: 1475-2875.
Tayou, T. C., Mbanya, D., Garraud, O., & Lefrère, J. J. (2007). [Blood safety: malaria and blood donation in Africa]. Transfusion clinique et biologique: journal de la Societe francaise de transfusion sanguine, 2007; 14: 481-486.
Bates, I., Owusu-Ofori, A. K., & Parry, C. Malaria in the blood supply in Africa: Is it a problem? Vox Sanguinis, 2011; 101: 55-55.
Kátia Luz Torres, Mônica Nascimento dos Santos Moresco, Luciane Rodrigues Sales, Josilene da Silva Abranches, Márcia Almeida Araújo Alexandre and Adriana Malheiro. Transfusion-transmitted malaria in endemic zone: epidemiological profile of blood donors at the Fundação HEMOAM and use of rapid diagnostic tests for malaria screening in Manaus. Rev. Bras. Hematol. Hemoter. 2014; 36: 269–274.
Weekley, C., & Smith, D. S. Malaria: the clinical basics. Global Health Education Consortium. 2013.
Joel C. Mouatcho and J. P. Dean Goldring. Malaria rapid diagnostic tests: challenges and prospects. Journal of Medical Microbiology, 2013; 62: 1491–1505.
Chou, M., Kim, S., Khim, N., Chy, S., Sum, S., Dourng, D & Ménard, D. (2012). Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESS®), a new malaria rapid diagnostic test for detection of symptomatic malaria infections. Malaria Journal, 2012; 11: 10-1186.
Kakkilaya, B. S. Are We Treating Malaria? World, 1990: 3.
Li, Y., Kumar, N., Gopalakrishnan, A., Ginocchio, C., Manji, R., Bythrow, M, Kong, H. Detection and species identification of malaria parasites by isothermal tHDA amplification directly from human blood without sample preparation. The Journal of Molecular Diagnostics, 2013; 15: 634-641.
Mali, S., Kachur, S. P., Arguin, P. M. Malaria surveillance—United States, 2010. MMWR Surveill Summ, 2012; 61: 1.
Johnston, S. P., Pieniazek, N. J., Xayavong, M. V., Slemenda, S. B., Wilkins, P. P., da Silva, A. J. PCR as a confirmatory technique for laboratory diagnosis of malaria. Journal of clinical microbiology, 2006; 44: 1087-1089.
Lund, T. C., Hume, H., Allain, J. P., McCullough, J., Dzik, W. The blood supply in Sub-Saharan Africa: Needs, challenges, and solutions. Transfusion and Apheresis Science, 2013; 49: 416-421.
Bhutta, Z. A., Chopra, M., Axelson, H., Berman, P., Boerma, T., Bryce, J & Wardlaw, T. (2010). Countdown to 2015 decade report (2000–10): taking stock of maternal, newborn, and child survival. The Lancet, volume 375 (9730), pp2032-2044.
Ali, M. S. M., Kadaru, A. A. G. M. Y., Mustafa, M. S. (2004). Screening blood donors for malaria parasite in Sudan. Ethiopian Journal of Health Development, 2004; 18: 70-74.
Endeshaw, T., Gebre, T., Ngondi, J., Graves, P. M., Shargie, E. B., Ejigsemahu, Y., et al. Evaluation of light microscopy and rapid diagnostic test for the detection of malaria under operational field conditions: a household survey in Ethiopia. Malaria journal, 2008; 7: 118.
Gatti, M. Gramegna, Z. Bisoffi, A. Raglio1, M. Gulletta, C. Klersy, A. et al., A comparison of three diagnostic techniques for malaria rapid diagnostic test (NOWH Malaria), PCR and microscopy. Annals of tropical medicine and parasitology, 2007; 101: 195-204.
Bahadur, S., Pujani, M., Jain, M. Use of rapid detection tests to prevent transfusion-transmitted malaria in India. Asian journal of transfusion science, 2010; 4: 140.
Akotet, B. M. K., Nkare, C. A., Mbouoronde, O. C., Mawili-Mboumba, D. P. (2014). Journal. Malaria Chemoth Cont Elimination, 2014; 3: 125
Chanda, P., Hamainza, B., Moonga, H. B., Chalwe, V., Pagnoni, F. (2011). Community case management of malaria using ACT and RDT in two districts in Zambia: achieving high adherence to test results using community health workers. Malaria Journal, 2011; 10: 158.
Asante, K. P., Abokyi, L., Zandoh, C., Owusu, R., Awini, E., Sulemana, A et al. Community perceptions of malaria and malaria treatment behaviour in a rural district of Ghana: implications for artemisinin combination therapy. Public Health, 2010; 10: 409.
Daniel Eibach, Boubacar Traore, Mourad Bouchrik, Boubacar Coulibaly, Nianégué Coulibaly, Fanta Siby, Guillaume Bonnot, Anne-Lise Bienvenu and Stéphane Picot. Evaluation of the malaria rapid diagnostic test VIKIA malaria Ag Pf/Pan™ in endemic and non-endemic settings. Malaria Journal, 2013; 12: 188.
Masanja, I. M., Selemani, M., Amuri, B., Kajungu, D., Khatib, R., Kachur, S. P., & Skarbinski, J. (2012). Increased use of malaria rapid diagnostic tests improves targeting of anti-malarial treatment in rural Tanzania: implications for nationwide rollout of malaria rapid diagnostic tests. Malaria Journal, 2012; 11: 221.
Mens, P., Spieker, N., Omar, S., Heijnen, M., Schallig, H. D. F. H., Kager, P. A. Is molecular biology the best alternative for diagnosis of malaria to microscopy? A comparison between microscopy, antigen detection and molecular tests in rural Kenya and urban Tanzania. Tropical Medicine & International Health, 2007; 12: 238-244.
Newman, R. D. World Malaria Report 2011. 2012.
Gerstl, S., Dunkley, S., Mukhtar, A., De Smet, M., Baker, S., Maikere, J. Assessment of two malaria rapid diagnostic tests in children under five years of age, with follow-up of false-positive pLDH test results, in a hyperendemic falciparum malaria area. Sierra Leone. Malaria Journal, 2010; 9: 28.
Zhao, J., Lama, M., Korenromp, E., Aylward, P., Shargie, E., Filler, S & Atun, R. (2012). Adoption of rapid diagnostic tests for the diagnosis of malaria, a preliminary analysis of the global fund program data, 2005 to 2010. PloS one, 2012; 7: e43549.
Ansah, E. K., Narh-Bana, S., Affran-Bonful, H., Bart-Plange, C., Cundill, B., Gyapong, M., et al. The impact of providing rapid diagnostic malaria tests on fever management in the private retail sector in Ghana: a cluster randomized trial. Malaria journal, 2015; 350: 1019.
Reyburn, H., Mbakilwa, H., Mwangi, R., Mwerinde, O., Olomi, R., Drakeley, C., et al. Rapid diagnostic tests compared with malaria microscopy for guiding outpatient treatment of febrile illness in Tanzania: randomised trial. Biomedical journal, 2007; 334: 403.
Boyce, R. M., Muiru, A., Reyes, R., Ntaro, M., Mulogo, E., Matte, M., & Siedner, M. J. (2015). Impact of rapid diagnostic tests for the diagnosis and treatment of malaria at a peripheral health facility in Western Uganda: an interrupted time series analysis. Malaria Journal, 2015; 14: 203.
Hendriksen, I. C., White, L. J., Veenemans, J., Mtove, G., Woodrow, C., Amos, B etal. Defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma PfHRP2 concentration. Journal of Infectious Diseases, 2013; 207: 351-361.
Lassina, Ouattara KLoudou, Jean & Cheikna, Zongo & N, Barro & Savadogo, Aly & Bassolé, Imael & A. S, Ouattara & Traore, Alfred. (2011). Antioxidant and Antibacterial Activities of Three Species of Lannea from Burkina Faso. Journal of Applied Sciences. 11.10.3923/jas.2011.157.162.
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