Bioassay Guided Phyto-chemical Investigation of <i>Bergenia ciliata (Haw) Sternb</i>: A Rocky Himalayan Medicinal Plant of Nepal

Pujya Wagle; Birendra Tamrakar; Swagat Shrestha; Lekh Nath Gautam

doi:doi:10.11648/j.jddmc.20160202.11

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Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal

Pujya Wagle, Birendra Tamrakar, Swagat Shrestha, Lekh Nath Gautam

Published in Journal of Drug Design and Medicinal Chemistry (Volume 2, Issue 2)

Received: 23 January 2016 Accepted: 9 February 2016 Published: 14 April 2016

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Abstract

Bergenia ciliata (Haw) Sternb. (Saxifragaceae) is a promising, but rare and vulnerable Himalayan medicinal herb of Nepal. It grows on moist crevices of rocks and bounders. Commonly, it is known as pashanved (pasan = rock stone, ved = piercing) or rock foil. It grows between the rocks possessing lithotropic property. A decoction of rhizomes and roots of this species is used in the Ayurveda, Unani and folk systems of medicine for the treatment of ulcers, fevers, tumors, eye sores and lungs, liver, heart, urinary diseases. Due to over-exploitation for medicinal value, it is in the process of extinction. So conservation by cultivation is highly recommended. The present study was carried out on bio-guided fractionation of rhizomes powder of B. ciliata (Sternb) which is known to possess several pharmacological properties. It afforded the bioactive natural product bergenin (Isocoumarin, 3%) from ethyl acetate fraction. In addition, other four compounds namely afzelechin (2.5%), leucocyanidin, β-sitosterol and β-sitosterol glycoside were isolated from ethyl acetate fraction. co-TLC/2D-TLC, HRMS, LC-MS, NMR, IR methods were employed to identify these compounds.

Published in	Journal of Drug Design and Medicinal Chemistry (Volume 2, Issue 2)
DOI	10.11648/j.jddmc.20160202.11
Page(s)	21-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bergenia ciliata, Saxifragaceae, Rhizomes, Chemical Constituents, Bergenin, Afzelechin, Leucocyanidin, β-sitosterol Glycoside

References

[1]	Rajbhandari, M.; Wegner, U.; Schopke, T.; Lindequist, U.; Mentel, R. Inhibitory effect of Bergenia ligulata on influenza virus A. Pharmazie, 2003, 58, 268-271.
[2]	Shah, M. C.; Joshi, M. C. An Ethnobotanical study of Kumaun Region of India Econ. Bot. 1971, 25, 414-422.
[3]	Manandhar. N. P. Plants and People of Nepal Timber Press. Oregon. 2002, ISBN 0-88192-527-6.
[4]	Bahl, C. P.; Murari, R.; Parthasarathy, M. R.; Seshadri, T. R. Components of Bergenia stracheyi and B. ligulata. Indian J chem., 1974, 12(10), 1038.
[5]	Bohm, B. A.; Donevan, L. S.; Bhat, U. G. Flavonoids of some species of Bergenia. Francoa, Parnassia and Lepuropetalon. Biochem Syst Ecol, 1986, 14(1), 75-77.
[6]	Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobson, L. B., Nichols, D. E., and McLaughlin, J. L.. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 1982, 45, 31-34.
[7]	Seeley, H. W.; Van Denmark, P. J. A Laboratory manual of Microbiology. 2nd ed. Bombay: D B. Taraporewala Sons and Co; 1975. Microbes in action; pp. 55–80.
[8]	Nazir, N.; Koul, S.; Qurishi, M. A.; Najar, M. H.; Zargar, M. I. Evaluation of antioxidant and antimicrobial activities of Bergenin and its derivatives obtained by chemoenzymatic synthesis Eur. J. Med. Chem. 2011, 46(6), 2415-2420.
[9]	Ogan A. U. Humiriaceae. An isocoumarin from the bark of Sacoglttis gabonensis. Photochemistry. 1971, 10, 2832-2833.
[10]	Yoshida, T.; Seno, K.; Takama, Y.; Okuda, T. Bergenin derivatives from Mallotus japonicas Phytochemistry 1982, 21, 1180-1182.
[11]	Haslam E. (+)-catechin-3-gallate and a polymeric proanthocyanidin from Bergenia species. J Chem Soc Perkin 1 1969, 14, 1824-1828.
[12]	Gautam, L. N.; Awale, S; Kalauni, S. K.; Shrestha, K. & Gewali, M. B. Phytochemical and Biological Studies on Podocarpus nerifolius D. Don, A Himalayan Conifer of Nepal Scientific World, 2005, 3(3), 22-25.

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Pujya Wagle, Birendra Tamrakar, Swagat Shrestha, Lekh Nath Gautam. (2016). Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. Journal of Drug Design and Medicinal Chemistry, 2(2), 21-25. https://doi.org/10.11648/j.jddmc.20160202.11

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ACS Style

Pujya Wagle; Birendra Tamrakar; Swagat Shrestha; Lekh Nath Gautam. Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. J. Drug Des. Med. Chem. 2016, 2(2), 21-25. doi: 10.11648/j.jddmc.20160202.11

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AMA Style

Pujya Wagle, Birendra Tamrakar, Swagat Shrestha, Lekh Nath Gautam. Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. J Drug Des Med Chem. 2016;2(2):21-25. doi: 10.11648/j.jddmc.20160202.11

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@article{10.11648/j.jddmc.20160202.11,
  author = {Pujya Wagle and Birendra Tamrakar and Swagat Shrestha and Lekh Nath Gautam},
  title = {Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {2},
  number = {2},
  pages = {21-25},
  doi = {10.11648/j.jddmc.20160202.11},
  url = {https://doi.org/10.11648/j.jddmc.20160202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20160202.11},
  abstract = {Bergenia ciliata (Haw) Sternb. (Saxifragaceae) is a promising, but rare and vulnerable Himalayan medicinal herb of Nepal. It grows on moist crevices of rocks and bounders. Commonly, it is known as pashanved (pasan = rock stone, ved = piercing) or rock foil. It grows between the rocks possessing lithotropic property. A decoction of rhizomes and roots of this species is used in the Ayurveda, Unani and folk systems of medicine for the treatment of ulcers, fevers, tumors, eye sores and lungs, liver, heart, urinary diseases. Due to over-exploitation for medicinal value, it is in the process of extinction. So conservation by cultivation is highly recommended. The present study was carried out on bio-guided fractionation of rhizomes powder of B. ciliata (Sternb) which is known to possess several pharmacological properties. It afforded the bioactive natural product bergenin (Isocoumarin, 3%) from ethyl acetate fraction. In addition, other four compounds namely afzelechin (2.5%), leucocyanidin, β-sitosterol and β-sitosterol glycoside were isolated from ethyl acetate fraction. co-TLC/2D-TLC, HRMS, LC-MS, NMR, IR methods were employed to identify these compounds.},
 year = {2016}
}

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TY  - JOUR
T1  - Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal
AU  - Pujya Wagle
AU  - Birendra Tamrakar
AU  - Swagat Shrestha
AU  - Lekh Nath Gautam
Y1  - 2016/04/14
PY  - 2016
N1  - https://doi.org/10.11648/j.jddmc.20160202.11
DO  - 10.11648/j.jddmc.20160202.11
T2  - Journal of Drug Design and Medicinal Chemistry
JF  - Journal of Drug Design and Medicinal Chemistry
JO  - Journal of Drug Design and Medicinal Chemistry
SP  - 21
EP  - 25
PB  - Science Publishing Group
SN  - 2472-3576
UR  - https://doi.org/10.11648/j.jddmc.20160202.11
AB  - Bergenia ciliata (Haw) Sternb. (Saxifragaceae) is a promising, but rare and vulnerable Himalayan medicinal herb of Nepal. It grows on moist crevices of rocks and bounders. Commonly, it is known as pashanved (pasan = rock stone, ved = piercing) or rock foil. It grows between the rocks possessing lithotropic property. A decoction of rhizomes and roots of this species is used in the Ayurveda, Unani and folk systems of medicine for the treatment of ulcers, fevers, tumors, eye sores and lungs, liver, heart, urinary diseases. Due to over-exploitation for medicinal value, it is in the process of extinction. So conservation by cultivation is highly recommended. The present study was carried out on bio-guided fractionation of rhizomes powder of B. ciliata (Sternb) which is known to possess several pharmacological properties. It afforded the bioactive natural product bergenin (Isocoumarin, 3%) from ethyl acetate fraction. In addition, other four compounds namely afzelechin (2.5%), leucocyanidin, β-sitosterol and β-sitosterol glycoside were isolated from ethyl acetate fraction. co-TLC/2D-TLC, HRMS, LC-MS, NMR, IR methods were employed to identify these compounds.
VL  - 2
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Author Information

Pujya Wagle

Nepal Academy of Science and Technology, Khumaltar, Nepal
Birendra Tamrakar

Tri-Chandra Multiple Campus, Department of Chemistry, Ghantaghar, Kathmandu, Nepal
Swagat Shrestha

Kathmandu Valley College, Chhauni, Nepal
Lekh Nath Gautam

Department of Plant Resources, Natural Product Research Laboratory (NPRL), Thapathali, Nepal

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APA Style

Pujya Wagle, Birendra Tamrakar, Swagat Shrestha, Lekh Nath Gautam. (2016). Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. Journal of Drug Design and Medicinal Chemistry, 2(2), 21-25. https://doi.org/10.11648/j.jddmc.20160202.11

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ACS Style

Pujya Wagle; Birendra Tamrakar; Swagat Shrestha; Lekh Nath Gautam. Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. J. Drug Des. Med. Chem. 2016, 2(2), 21-25. doi: 10.11648/j.jddmc.20160202.11

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AMA Style

Pujya Wagle, Birendra Tamrakar, Swagat Shrestha, Lekh Nath Gautam. Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal. J Drug Des Med Chem. 2016;2(2):21-25. doi: 10.11648/j.jddmc.20160202.11

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@article{10.11648/j.jddmc.20160202.11,
  author = {Pujya Wagle and Birendra Tamrakar and Swagat Shrestha and Lekh Nath Gautam},
  title = {Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {2},
  number = {2},
  pages = {21-25},
  doi = {10.11648/j.jddmc.20160202.11},
  url = {https://doi.org/10.11648/j.jddmc.20160202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20160202.11},
  abstract = {Bergenia ciliata (Haw) Sternb. (Saxifragaceae) is a promising, but rare and vulnerable Himalayan medicinal herb of Nepal. It grows on moist crevices of rocks and bounders. Commonly, it is known as pashanved (pasan = rock stone, ved = piercing) or rock foil. It grows between the rocks possessing lithotropic property. A decoction of rhizomes and roots of this species is used in the Ayurveda, Unani and folk systems of medicine for the treatment of ulcers, fevers, tumors, eye sores and lungs, liver, heart, urinary diseases. Due to over-exploitation for medicinal value, it is in the process of extinction. So conservation by cultivation is highly recommended. The present study was carried out on bio-guided fractionation of rhizomes powder of B. ciliata (Sternb) which is known to possess several pharmacological properties. It afforded the bioactive natural product bergenin (Isocoumarin, 3%) from ethyl acetate fraction. In addition, other four compounds namely afzelechin (2.5%), leucocyanidin, β-sitosterol and β-sitosterol glycoside were isolated from ethyl acetate fraction. co-TLC/2D-TLC, HRMS, LC-MS, NMR, IR methods were employed to identify these compounds.},
 year = {2016}
}

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TY  - JOUR
T1  - Bioassay Guided Phyto-chemical Investigation of Bergenia ciliata (Haw) Sternb: A Rocky Himalayan Medicinal Plant of Nepal
AU  - Pujya Wagle
AU  - Birendra Tamrakar
AU  - Swagat Shrestha
AU  - Lekh Nath Gautam
Y1  - 2016/04/14
PY  - 2016
N1  - https://doi.org/10.11648/j.jddmc.20160202.11
DO  - 10.11648/j.jddmc.20160202.11
T2  - Journal of Drug Design and Medicinal Chemistry
JF  - Journal of Drug Design and Medicinal Chemistry
JO  - Journal of Drug Design and Medicinal Chemistry
SP  - 21
EP  - 25
PB  - Science Publishing Group
SN  - 2472-3576
UR  - https://doi.org/10.11648/j.jddmc.20160202.11
AB  - Bergenia ciliata (Haw) Sternb. (Saxifragaceae) is a promising, but rare and vulnerable Himalayan medicinal herb of Nepal. It grows on moist crevices of rocks and bounders. Commonly, it is known as pashanved (pasan = rock stone, ved = piercing) or rock foil. It grows between the rocks possessing lithotropic property. A decoction of rhizomes and roots of this species is used in the Ayurveda, Unani and folk systems of medicine for the treatment of ulcers, fevers, tumors, eye sores and lungs, liver, heart, urinary diseases. Due to over-exploitation for medicinal value, it is in the process of extinction. So conservation by cultivation is highly recommended. The present study was carried out on bio-guided fractionation of rhizomes powder of B. ciliata (Sternb) which is known to possess several pharmacological properties. It afforded the bioactive natural product bergenin (Isocoumarin, 3%) from ethyl acetate fraction. In addition, other four compounds namely afzelechin (2.5%), leucocyanidin, β-sitosterol and β-sitosterol glycoside were isolated from ethyl acetate fraction. co-TLC/2D-TLC, HRMS, LC-MS, NMR, IR methods were employed to identify these compounds.
VL  - 2
IS  - 2
ER  -

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