LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract
Journal of Drug Design and Medicinal Chemistry
Volume 3, Issue 2, April 2017, Pages: 18-26
Received: Mar. 14, 2017; Accepted: Mar. 27, 2017; Published: Apr. 18, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Ariadne Esmene Afaganis, Trivedi Global, Inc., Henderson, Nevada, USA
Barbara Marie Bader, Trivedi Global, Inc., Henderson, Nevada, USA
Brian A. Weekes, Trivedi Global, Inc., Henderson, Nevada, USA
Daphne Luisa Dumas, Trivedi Global, Inc., Henderson, Nevada, USA
Denise Marie Fiedler, Trivedi Global, Inc., Henderson, Nevada, USA
Dennille Mellesia Smith, Trivedi Global, Inc., Henderson, Nevada, USA
Desi Pano, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Felice Galla, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Maria Alija, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine Barbara Mullins, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine M. Scorza, Trivedi Global, Inc., Henderson, Nevada, USA
Ellia O'Donnell, Trivedi Global, Inc., Henderson, Nevada, USA
Fabio Massimo Paciucci, Trivedi Global, Inc., Henderson, Nevada, USA
Frances Goodman Warlick, Trivedi Global, Inc., Henderson, Nevada, USA
Haddon Norman Salt, Trivedi Global, Inc., Henderson, Nevada, USA
Inthirani Arul, Trivedi Global, Inc., Henderson, Nevada, USA
Jacqueline Y. Andrews, Trivedi Global, Inc., Henderson, Nevada, USA
James Jay McLeran, Trivedi Global, Inc., Henderson, Nevada, USA
James Stephen Burnett, Trivedi Global, Inc., Henderson, Nevada, USA
Jean Caroline White, Trivedi Global, Inc., Henderson, Nevada, USA
Parthasarathi Panda, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Kalyan Kumar Sethi, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
Withania somnifera root extract which contains withanolides as major active constituent and it is used traditionally for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® - Consciousness Energy Healing Treatment on the structural properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents at respective retention time was significantly altered. The peak area% at Rt of 5.6, 6.8, 6.9, 7.2, 7.9, 8.4, 8.5, 8.6, and 9.2 minutes of the treated sample were increased significantly in the range of 1.46% to 253.06% compared to the control sample. In the contrary, the peak area% of the treated sample at Rt of 6.4 and 8.2 minutes were significantly decreased by 12.72% and 17.35%, respectively with respect to the control sample. A total of 16 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 621, 489, 473, 767, 471, 505, and 992 at retention times of 6.4, 6.8, 7.2, 7.9, 8.2, 8.4, and 9.1 minutes, respectively with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensities of the Biofield Energy Treated sample were significantly changed in the range of -81.36% to 1720.90% compared with the control sample at the same retention time. These findings suggest that The Trivedi Effect® - Consciousness Energy Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Keywords
Ashwagandha, Biofield Energy Healers, The Trivedi Effect®, Consciousness Energy Healing Treatment, Biofield Energy Healing Treatment, LC-MS, Withanolides, GC-MS
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana, LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract, Journal of Drug Design and Medicinal Chemistry. Vol. 3, No. 2, 2017, pp. 18-26. doi: 10.11648/j.jddmc.20170302.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Kesarwani K, Gupta R (2013) Bioavailability enhancers of herbal origin: An overview. Asian Pac J Trop Biomed 3: 253-266.
[2]
Singh N, Bhalla M, Jager P, Gilca M (2011) An overview on ashwagandha: A rasayana (rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[3]
Kulkarni SK, Dhir A (2008) Withania somnifera: An Indian ginseng. Prog Neuropsychopharmacol Biol Psychiatry 32: 1093-1105.
[4]
Dar NJ, Hamid A, Ahmad M (2015) Pharmacologic overview of Withania somnifera, the Indian ginseng. Cell Mol Life Sci 72: 4445-4460.
[5]
Mir BA, Khazir J, Mir NA, Hasan T-ul, Koul S (2012) Botanical, chemical and pharmacological review of Withania somnifera (Indian ginseng): An Ayurvedic medicinal plant. Indian J Drugs Dis 1: 147-160.
[6]
Mishra LC, Singh BB, Dagenais S (2000) Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A review. Altern Med Rev 5: 334-346.
[7]
Kumar V, Dey A, Hadimani MB, Marcović T, Emerald M (2015) Chemistry and pharmacology of Withania somnifera: An update. Tang (Humanitas Medicine) 5: e1.
[8]
Bolleddula J, Fitch W, Vareed SK, Nair MG (2012) Identification of metabolites in Withania sominfera fruits by liquid chromatography and high-resolution mass spectrometry. Rapid Commun Mass Spectrom 26: 1277-1290.
[9]
Mirjalili MH, Moyano E, Bonfill M, Cusido RM, Palazón J (2009) Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 14: 2373-2393.
[10]
Singh A, Duggal S, Singh H, Singh J, Katekhaye S (2010) Withanolides: Phytoconstituents with significant pharmacological activities. Int J Green Pharm 4: 229-237.
[11]
Chen LX, He H, Qiu F (2011) Natural withanolides: An overview. Nat Prod Rep 28: 705-740.
[12]
Budhiraja RD, Krishan P, Sudhir S (2000) Biological activity of withanolides. J Sci Ind Res 59: 904-911.
[13]
Stenger VJ (1999) Bioenergetic fields. Sci Rev Alternative Med 3.
[14]
Rogers, M (1989) "Nursing: A Science of Unitary Human Beings." In J. P. Riehl-Sisca (ed.) Conceptual Models for Nursing Practice. 3rd edition. Norwark: Appleton & Lange.
[15]
Rosa L, Rosa E, Sarner L, Barrett S (1998) A close look at therapeutic touch. JAMA 279: 1005-1010.
[16]
Warber SL, Cornelio D, Straughn, J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
[17]
Nelson LA, Schwartz GE (2005) Human biofield and intention detection: Individual differences. J Altern Complement Med 11: 93-101.
[18]
Nemeth L (2008) Energy and biofield therapies in practice. Beginnings. Summer 28: 4-5.
[19]
Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5: 18-20.
[20]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[21]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Bacterial identification using 16S rDNA gene sequencing and antibiogram analysis on biofield treated Pseudomonas fluorescens. Clin Med Biochemistry Open Access 1: 101.
[22]
Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015) Effect of biofield treatment on physical, thermal, and spectral properties of SFRE 199-1 mammalian cell culture medium. Advances in Biochemistry 3: 77-85.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
[24]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[25]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[26]
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Effect of biofield energy treatment on Streptococcus group B: A postpartum pathogen. J Microb Biochem Technol 7: 269-273.
[27]
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Effect of biofield treatment on antimicrobials susceptibility pattern of Acinetobacter baumannii - An Experimental Study. J Clin Diagn Res 3: 1.
[28]
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[29]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[30]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
[31]
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
[32]
Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
[33]
Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015) Spectroscopic characterization of disodium hydrogen orthophosphate and sodium nitrate after biofield treatment. J Chromatogr Sep Tech 6: 282.
[34]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
[35]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5: 414.
[36]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[37]
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[38]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[39]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[40]
Chatterjee S, Srivastava S, Khalid A, Singh N, Sangwan RS, Sidhu OP, Roy R, Khetrapal CL, Tuli R (2010) Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts. Phytochemistry 71: 1085-1094.
[41]
Chaurasiya ND, Uniyal GC, Lal P, Misra L, Sangwan NS, Tuli R, Sangwan RS (2008) Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal 19: 148-154.
[42]
Musharraf SG, Ali A, Ali RA, Yousuf S, Rahman AU, Choudhary MI (2011) Analysis and development of structure-fragmentation relationships in withanolides using an electrospray ionization quadropole time-of-flight tandem mass spectrometry hybrid instrument. Rapid Commun Mass Spectrom 25: 104-114.
[43]
Trivedi MK, Panda P, Sethi KK, Jana S (2016) Metabolite profiling of Withania somnifera roots hydroalcoholic extract using LC-MS, GC-MS and NMR spectroscopy. Chem Biodivers (Article in press) doi:10.1002/cbdv.201600280.
[44]
https://www.scribd.com/document/220440935/Theory-of-HPLC-Quantitative-and-Qualitative-HPLC.
[45]
Mulabagal V, Subbaraju GV, Rao CV, Sivaramakrishna C, Dewitt DL, Holmes D, Sung B, Aggarwal BB, Tsay HS, Nair MG (2009) Withanolide sulfoxide from Aswagandha roots inhibits nuclear transcription factor-B, cyclooxygenase and tumor cell proliferation. Phytother Res 23: 987-992.
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