Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations
International Journal of Pharmacy and Chemistry
Volume 3, Issue 4, July 2017, Pages: 52-55
Received: Jun. 1, 2017;
Accepted: Jun. 16, 2017;
Published: Jul. 21, 2017
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Idrees F. Al-Momani, Chemistry Department, Yarmouk University, Irbid, Jordan
Majdoleen H. Rababah, Chemistry Department, Yarmouk University, Irbid, Jordan
A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.
Idrees F. Al-Momani,
Majdoleen H. Rababah,
Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations, International Journal of Pharmacy and Chemistry.
Vol. 3, No. 4,
2017, pp. 52-55.
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