Antimicrobial Activity and Phytochemical Analysis of Medicinal Plant Cassia tora
International Journal of Pharmacy and Chemistry
Volume 3, Issue 4, July 2017, Pages: 56-61
Received: Jun. 22, 2017; Accepted: Aug. 3, 2017; Published: Sep. 6, 2017
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Mithilesh P. Pandya, Department of Chemistry. C. U. Shah University, Wadhwan, India
Krunal D. Sameja, Department of Chemistry. C. U. Shah University, Wadhwan, India
Dixita N. Patel, Department of Chemistry. C. U. Shah University, Wadhwan, India
Keyur D. Bhatt, Department of Chemistry, Ganpat University, Kherva, India
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Present study was carried out to investigate in vitro anti-bacterial and anti-fungal activity from the seeds of an Indian traditional medicinal plant Cassia tora. Plant material was separated and successively extracted with various organic solvents. Extracts was evaluated for solubility, moisture content, melting point, FTIR and other qualitative analysis for Photo constituents. In vitro antibacterial and antifungal studies were carried out by disc diffusion method. Two test samples were prepared in concentrations of 100mg/ml. Extract was found efficacious against various strains of bacteria and fungal species. 100mg/ml test sample shows better zone of inhibitions in bacterial strains. It was obtained in the range of 16.67 to 23.00 among them maximum inhibition was observed in pseudomonasaeruginosa (gram positive bacteria) and last inhibition was observed in E. coli (gram negative). It has also shown satisfactory inhibition in fungal species like Candida albicans and Candida glabrata. Thus, cassia tora can be considered as a safer and efficacious herbal candidate for antimicrobial formulation.
Cassia-tora, Vitro Study, Anti-bacterial, Anti-fungal, Isolation
To cite this article
Mithilesh P. Pandya, Krunal D. Sameja, Dixita N. Patel, Keyur D. Bhatt, Antimicrobial Activity and Phytochemical Analysis of Medicinal Plant Cassia tora, International Journal of Pharmacy and Chemistry. Vol. 3, No. 4, 2017, pp. 56-61. doi: 10.11648/j.ijpc.20170304.12
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Choi JS, Lee HJ, Park K-Y, Ha J-O, Kang SS. In vitro antimutagenic effects of anthraquinone aglycones and naphthopyrone glycosides from Cassia tora. Planta medica 1997; 63:11-4.
Ma JF, Zheng SJ, Matsumoto H. Specific secretion of citric acid induced by Al stress in Cassia tora L. Plant and Cell Physiology 1997;38:1019-25.
Maity TK, Mandal SC, Mukherjee PK, Saha K, Das J, Pal M, et al. Studies on antiinflammatory effect of Cassia tora leaf extract (fam. Leguminosae). Phytotherapy research 1998; 12:221-3.
Patil UK, Saraf S, Dixit V. Hypolipidemic activity of seeds of Cassia tora Linn. Journal of ethnopharmacology 2004;90:249-52.
Wang Y-S, Yang Z-M. Nitric oxide reduces aluminum toxicity by preventing oxidative stress in the roots of Cassia tora L. Plant and Cell Physiology 2005; 46:1915-23.
Kim Y-M, Lee C-H, Kim H-G, Lee H-S. Anthraquinones isolated from Cassia tora (Leguminosae) seed show an antifungal property against phytopathogenic fungi. Journal of agricultural and food chemistry 2004; 52:6096-100.
Mukherjee PK, Saha K, Saha B, Pal M, Das J. Antifungal activities of the leaf extract of Cassia tora Linn.(Fam. Leguminosae). Phytotherapy Research 1996; 10:521-2.
Hocking P, Pate J. Mobilization of minerals to developing seeds of legumes. Annals of Botany 1977; 41:1259-78.
Garcia-Martinez J-L, Sponsel V, Gaskin P. Gibberellins in developing fruits of Pisum sativum cv. Alaska: studies on their role in pod growth and seed development. Planta 1987; 170:130-7.
Lee YP, Puddey IB, Hodgson JM. Protein, fibre and blood pressure: potential benefit of legumes. Clinical and Experimental Pharmacology and Physiology 2008; 35:473-6.
Messina MJ. Legumes and soybeans: overview of their nutritional profiles and health effects. The American journal of clinical nutrition 1999; 70:439s-50s.
de Almeida Costa GE, da Silva Queiroz-Monici K, Reis SMPM, de Oliveira AC. Chemical composition, dietary fibre and resistant starch contents of raw and cooked pea, common bean, chickpea and lentil legumes. Food chemistry 2006; 94:327-30.
Jezierny D, Mosenthin R, Bauer E. The use of grain legumes as a protein source in pig nutrition: A review. Animal Feed Science and Technology 2010; 157:111-28.
Hargrove W. Winter legumes as a nitrogen source for no-till grain sorghum. Agronomy Journal 1986; 78:70-4.
Popkin BM, Du S. Dynamics of the nutrition transition toward the animal foods sector in China and its implications: a worried perspective. The Journal of nutrition 2003;133:3898S-906S.
Madar Z, Stark AH. New legume sources as therapeutic agents. British Journal of Nutrition 2002; 88:287-92.
Clemente A, Domoney C. Therapeutic properties of legume protease inhibitors from the Bowman–Birk class. Recent progress in medicinal plants 2007; 20:397-417.
Bruneau A, Forest F, Herendeen PS, Klitgaard BB, Lewis GP. Phylogenetic relationships in the Caesalpinioideae (Leguminosae) as inferred from chloroplast trnL intron sequences. Systematic Botany 2001; 26:487-514.
Sharma S, Dangi MS, Wadhwa S, Daniel V, Tiwari A. Antibacterial activity of Cassia tora leaves. Int J Pharmaceutical and Biological Archives 2010; 1:84-6.
Jenkins D, Wolever T, Bacon S, Nineham R, Lees R, Rowden R, et al. Diabetic diets: high carbohydrate combined with high fiber. The American journal of clinical nutrition 1980; 33:1729-33.
Lean M, James W. Prescription of diabetic diets in the 1980s. The Lancet 1986; 327:723-5.
Roopashree T, Dang R, Rani RS, Narendra C. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied research in Natural products 2008; 1:20-8.
Bhatt KD, Vyas DJ, Makwana BA, Darjee SM, Jain VK. Highly stable water dispersible calix [4] pyrrole octa-hydrazide protected gold nanoparticles as colorimetric and fluorometric chemosensors for selective signaling of Co (II) ions. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2014; 121:94-100.
Bhatt KD, Gupte HS, Makwana BA, Vyas DJ, Maity D, Jain VK. Calix receptor edifice; scrupulous turn off fluorescent sensor for Fe (III), Co (II) and Cu (II). Journal of fluorescence 2012; 22:1493-500.
Bhatt KD, Makwana BA, Vyas DJ, Mishra DR, Jain VK. Selective recognition by novel calix system: ICT based chemosensor for metal ions. Journal of Luminescence 2014; 146:450-7.
Bhatt KD, Vyas DJ, Makwana BA, Darjee SM, Jain VK, Shah H. Turn-on fluorescence probe for selective detection of Hg (II) by calixpyrrole hydrazide reduced silver nanoparticle: Application to real water sample. Chinese Chemical Letters 2016; 27:731-7.
Albasarah YY, Somavarapu S, Stapleton P, Taylor KM. Chitosan‐coated antifungal formulations for nebulisation. Journal of Pharmacy and Pharmacology 2010; 62:821-8.
Porter R. The Greatest Benefit to Mankind: A Medical History of Humanity (The Norton History of Science): WW Norton & Company; 1999.
Organization WH. European status report on road safety: towards safer roads and healthier transport choices.2009.
Anand K, Agrawal P, Kumar S, Kapila K. Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian journal of medical microbiology 2009; 27:27.
Ho P, Chow K, Yuen K, Ng W, Chau P. Comparison of a novel, inhibitor-potentiated disc-diffusion test with other methods for the detection of extended-spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae. Journal of Antimicrobial Chemotherapy 1998; 42:49-54.
Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology 2000; 31:247-56.
Ali‐Shtayeh M, Abu Ghdeib SI. Antifungal activity of plant extracts against dermatophytes. mycoses 1999; 42:665-72.
Hammer KA, Carson C, Riley T. Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology 1999; 86:985-90.
Ayoola G, Coker H, Adesegun S, Adepoju-Bello A, Obaweya K, Ezennia E, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research 2008; 7:1019-24.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Internationale pharmaceutica sciencia 2011; 1:98-106.
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