Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets
International Journal of Pharmacy and Chemistry
Volume 5, Issue 4, July 2019, Pages: 42-47
Received: Apr. 5, 2019;
Accepted: Jul. 22, 2019;
Published: Oct. 9, 2019
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Imad Osman Abu Reid, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National Al Ribat University, Khartoum, Sudan
Malak Elrasheed Mohamed, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National Al Ribat University, Khartoum, Sudan
A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.
Imad Osman Abu Reid,
Malak Elrasheed Mohamed,
Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets, International Journal of Pharmacy and Chemistry.
Vol. 5, No. 4,
2019, pp. 42-47.
Cardiovascular Drugs. In Martindale, the Complete Drug Reference, Edited by Sweetman SC. Pharmaceutical Press, London. 2009; 36th Edn: 1214.
Cardiovascular Drugs. In Martindale, the complete drug reference, Edited bySweetman SC. Pharmaceutical Press, London. 2009; 36th Edn: 1218-1219.
McKeage K and Siddiqui MA. Amlodipine/Atorvastatin Fixed-Dose Combination: A Review of its Use in the Prevention of Cardiovascular Disease and in the Treatment of Hypertension and Dyslipidemia. Am. J. Cardiovasc. Drugs 2008; 8: 51–67.
Kamal AH, El-Malla SF and Hammad SF. A review on UV spectrophotometric methods for simultaneous multicomponent analysis. Euro. J. Pharm. Med. Res. 2016; 3 (2): 348-360.
Lotfy HMand Saleh SS. Recent development in ultraviolet spectrophotometry through the last decade (2006–2016): a review. Int. J. Pharm. Pharmac. Sci. 2016; 8 (10): 40-56
Abdallah OM and Badawey AM. Derivative- Ratio Spectrophotometric, Chemometric and HPLC Validated methods for Simultaneous Determination of Amlodipine and Atorvastatin in Combined Dosage Form. Int. J. Ind. Chem. 2011; 2 (2): 78-85.
Darwish HW, Hassan SA and Salem MY, B AEl-Zeand. Development and Validationof H-point Standard Addition Method Appliedforthe Analysisof BinaryMixtrue ofAmlodipineand Atorvastatin. Int. J. Pharm. Bio. Sci. 2013; 4 (2): 230- 243.
Sarrafi AHM, KonozE and Ghiyasvand M. Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate CalibrationMethods in Pharmaceutical Formulations. E-Journal of Chemistry2011; 8 (4): 1670-1679.
Silvia I, Klára K and Daniela-Luci M. Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets byApparent Content Curve and HPLC Methods. Acta Medica Marisiensis 2013; 59 (1): 44-48.
Bernard S, Mathew M, Senthilkumar KL and Girija KN. Simultaneous Estimation of Atorvastatin Calcium and Amlodipine besylate by UV Spectrophotometric method using hydrotropic solubilization. Hygeia. J. D. Med. 2013; 5 (1): 105-112.
Sahu R and Patel BV. Simultaneous Spectrophotometric Determination of Amlodipine besylate and Atorvastatin calcium in Binary Mixture. Indian J. Pharm. Sci. 2007; 69 (1): 110-111.
Hmadi FA and Am GR. Spectrophotometric Determination of Atorvastatin and Amlodipine by H-point Stndard Addition Methodwith Simultaneous Addition of Both Analalytes in Nonaqueous Solution. Collect. Czech. Chem. Commun. 2011; 76 (3): 193–205.
Tehrani MB, Shoorvazi M and Souri E. Stability Indicating Derivative Spectrophotometric Method for Simultaneous Determination of Amlodipine and Atorvastatin in Pharmaceutical Dosage Forms. Research J. Pharm. Biol. Chem. Sci. 2014; 5 (3): 26-34.
Darwish HW, Hassan SA, Salem MY and El-Zeany BA. Three different methods for determination of binary mixture of Amlodipine and Atorvastatin using dual wavelength spectrophotometry. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2013; 104: 70–76.
Sharma K, Sharma Yand Sharma P. Validated Method Developmentfor Estimation of Atorvastatin and Amlodipine in Solid Dosage Regimens. Int. J. Res. Dev. Pharm. L. Sci. 2013; 2 (2): 344-348.
Jena A, Madhu M and Latha S. Analytical Method Developmentand Validation of Simultaneous Determination of Atorvastatin calcium and Amlodipine besylate in Tablet Dosage Form by RP-HPLC. IJPSR, 2010; 1 (11): 100-106.
Hassan SA, Elzanaly ES, Salem MY and EL-Zeany BA. Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatinin the presence of their acidic degradation products in tablets. Acta Pharm. 2016; 66: 479–490.
Rao NM and Sankar DG. Development and Validation of HPTLC Method for theSimultaneous Estimation of Amlodipine Besylate and Atorvastatin Calcium in Combined Dosage Form. Eurasian Journal of Analytical Chemistry 2016; 11 (3): 155-168.
Hafez HM, Elshanawany AA, Abdelaziz LM and Mohram MS. Development of a Stability-Indicating HPLC Method for Simultaneous Determination of Amlodipine Besylate and Atorvastatin Calcium in Tablets. Austin J Anal Pharm Chem. 2014; 1 (6): 1- 11.
Mathew M, Bernard S, Senthil Kumar KL and GirijaKN. Development and validation of a high performance thin layer chromatographic method for the simultaneous estimation of Atorvastatin calcium and Amlodipine besylate as the bulk drugs and in the tablet dosage form. Asian J. Pharm. Hea. Sci. 2014; 4 (2): 986-991.
Eranki RJV, Intil G and JayaramanV. New Stability Indicating Method for Quantification of Impurities in Amlodipine and Atorvastatin calcium Tablets by Validated HPLC. Int. J. Pharm. Res. Schol. 2013; 2: 458-471.
ToanPD, MinhPPAand Trucly DT. Novel HPLC-UV Method Using Volatile Buffer for Simultaneous Determination of Amlodipine Besylateand Atorvastatin Calcium. Int. J. of Pharm. Pharmac. Sci. 2016; 8 (7): 365-368.
Vidyadhara S, Sasidhar RLC, Satyaprasanna P, Aruna KC and Kumari AG. RP-HPLC method development for the simultaneous estimation ofatorvastatin and amlodipine besylate in bulk and pharmaceutical dosage forms. Der Pharmacia Sinica 2015; 6 (10): 1-7.
Moussa BA, El-Zaher A, Mahrouse MA and AhmedMS. simultaneous Determination of Amlodipine Besylate andAtorvastatin Calcium in Binary Mixture by Spectrofluorimetry and HPLC coupled with Fluorescence Detection. Analytical Chemistry Insights 2013; 8: 107-115.
IbrahimN, RizkM, IbrahimA, Tawakkol S and ALI I. Simultaneous Determination of Amlodipine Besylateand Atorvasrtatin Caciumby Using Spectrophotometric Method with Multivariate Calibration and HPLC Method Implementing “Design of Experiment”. Int J Pharm Pharm Sci. 2014; 6 (1): 419-425.
Ahmed M, Alshabrawy A and Nageh A. UPLC-MS/MS Method for Kinetic Studies and Simultaneous Determination of Amlodipine and Atorvastatin in Bulk, and Their Combined Dosage Form. Anal Chem Ind J. 2016; 16 (14): 102-115.
Dogan-Topal B, Bozal B, Demircigil BT, Uslu B and Ozkan SA. Electroanalytical Studies and Simultaneous Determination of Amlodipine Besylate and Atorvastatine Calcium in Binary Mixtures Using First Derivative of the Ratio-Voltammetric Methods. Electroanalysis 2009; 21 (22): 2427–2439.
Rebech I, Salamanca-Neto CAR, Scremin J and Sartori ER. Simultaneous Voltammetric Determination of Amlodipine andAtorvastatin on Anodically Pretreated Boron-Doped Diamond Electrode Orbital: Electron. J. Chem. 2017; 9 (4): 225-233.
MirciaE, Balaci T, HancuG, IonVand Carje A. Simultaneous Determination of Amlodipine and Atorvastatin by Capillary Electrophoresis from Fixed Pharmaceutical Formulations. Farmacia 2016; 64 (3): 398-402.
Al- Rasoul B, Elsamani T. Validated RP- - HPLC Method for the Simultaneous Determination of Amlodipine Besylate and Atorvastatin Calcium in Bulk and Pharmaceutical Formulation. International Journal of Research in Pharmacy and Chemistry. 2016; 6 (4): 816-825.
Brown CW, Lynch PF, Obremski RJ and Lavery DS. Matrix Representations and Criteria for Selecting Analytical Wavelengths for Multicomponent Spectroscopic Analysis Anal. Chem. 1982; 54: 1472-1479.