Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity
International Journal of Pharmacy and Chemistry
Volume 5, Issue 6, November 2019, Pages: 68-71
Received: Oct. 11, 2019;
Accepted: Oct. 29, 2019;
Published: Nov. 13, 2019
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Ivan Polischuk, Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine
Mykola Komisarenko, Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine
Taras Upyr, Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine
Alla Kovaleva, Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine
Andriy Komisarenko, Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine
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Medicines on the basis of herbs are increasingly used for treating of many diseases, providing the following actions antimicrobial, antiviral, anticancer, anti-enzymatic and immunomodulatory. Ellagitannins are a group of polyphenolic compounds of plant origin, which exhibiting a strong antibacterial effect in virtue of their ability to form a bond with the microbial membrane. Raspberry contains a large amount of ellagitannins, so it is advisable to study their composition and content in raspberry juice, obtained in Ukraine and determine its antimicrobial activity. As raw material Ukrainian variety ‘Phenomenon’ was taken for the research. It was bred in 1991 in Kharkiv region by crossing the varieties ‘Stolichnaya’ and ‘Odarka’. Analysis of raspberry juice ellagitannins was performed by HPLC method. 4 ellagic acid derivatives were identified and their contents were established. Unbound ellagic acid (55% of all) and sanguiin H-6 (21% of all) had the highest content among them Antimicrobial activity of raspberry juice was studied by agar diffusion method. The juice showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The antimicrobial activity of the juice was higher than the comparison drug " Chlorophyllipt " for more than twice in relation to all strains. The greatest effect was manifested relative to Staphylococcus aureus, which can be the cause of purulent-inflammatory processes in almost all human organs with severe consequences. This is especially true in connection with the increasing resistance of strains not only to traditional antibiotics, but also to new groups.
Raspberry, Juice, Ellagitannins, Antimicrobial Activity
To cite this article
Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity, International Journal of Pharmacy and Chemistry. Special Issue: Drug Research: Current Insights for Search, Development and Analysis.
Vol. 5, No. 6,
2019, pp. 68-71.
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Okuda T., Yoshida T., Hatano T. (1995). Progress in the chemistry of organic natural products. Springer: New York, NY, USA: 420 p.)
Haslam E. (1989). Plant Polyphenols. Cambridge University Press: Cambridge-UK: 273 p.
Okuda T., Yoshida T., Ashida M. (1981). Casuarictin and casuarinin, two new ellagitannins from Casuarina stricta. Heterocycles № 16: 1681–1685.
Kolodziej H., Kayser O., Kiderlen, A. F., Ito H., Hatano T., Yoshida T., Foo L. Y. (2001). Antileishmanial activity of hydrolyzable tannins and their modulatory effects on nitric oxide and tumor necrosis factor-α release in macrophages in vitro. Planta Med. № 67: 825–832.
Cheng H., Lin C. C., Lin T. C. (2002) Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antiviral Res. № 55: 447–455.
Po-Lin K., Ya-Ling H., Ta-Chen L., Liang-Tzung L., Jiunn Kae C., Chun-Ching L. (2005). Casuarinin from the bark of Terminalia arjuna induces apoptosis and cell cycle arrest in human breast adenocarcinoma MCF-7 cells. Planta Medica № 71: 237–243.
Reddy D. B., Reddanna P. (2009). Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-kappaB and MAPK activation in RAW 264.7 macrophages. Biochem. Biophys. Res. Commun № 381: 112–117.
Cushnie T. P. T., Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents № 26 (5): 343–356.
Chirumbolo S., Bjørklund G., Lysiuk R., Vella A., Lenchyk L., Upyr T. (2018). Targeting Cancer with Phytochemicals via Their Fine Tuning of the Cell Survival Signaling Pathways. International Journal of Molecular Sciences № 19 (11): 3568.
Pharmaceutical encyclopedia. 2nd ed.: Chairman Ed. Council Chernykh V. P. Kiev: “MORION”; 2016: 1952.
Zhang X., Sandhu A., Edirisinghe I., Burton-Freeman B. (2018). An exploratory study of red raspberry (Rubus idaeus L.) (poly) phenols/metabolites in human biological samples. Food & Function № 9 (2): 806–818.
Kula M., Głód D., Krauze-Baranowska M. (2016). Two-dimensional liquid chromatography (LC) of phenolic compounds from the shoots of Rubus idaeus “Glen Ample” cultivar variety. Journal of Pharmaceutical and Biomedical Analysis № 121: 99–106.
Zhbanova E. V. (2018). Fruit of raspberry Rubus idaeus L. as a source of functional ingredients (review) Food Processing: Techniques and Technology. Vol. 48. № 1: 5-14.
Sójka M., Macierzyński J., Zaweracz W., Buczek M. (2016). Transfer and Mass Balance of Ellagitannins, Anthocyanins, Flavan-3-ols, and Flavonols during the Processing of Red Raspberries (Rubus idaeus L.) to Juice. Journal of Agricultural and Food Chemistry №64 (27): 5549–5563.
Yeromina H. O., Upyr T. V., Osolodchenko T. P., Ieromina Z. G., Demchenko N. R., Perekhoda L. O. (2019). The search for substances with the antimicrobial activity among morpholine-containing 2-R-phenyliminothiazole derivatives. Journal of organic and pharmaceutical chemistry Vol. 17, Iss. 1 (65): 58–62.