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Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro

Received: 21 May 2020    Accepted: 15 June 2020    Published: 17 July 2020
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Abstract

Our study focused on “Extraction identification and characterization of saponins from the plant Myrica salicifolia Hochst. ex. A. Rich and molluscicidal activity test in vitro". The objective of this study is to extract the natural substances present in the different parts (leaves, bark of the stem and roots); isolate the saponins, characterize them by chromatography and determine the lethal dose of the saponins isolated against aquatic molluscs. After the analyzes, the Malacology laboratory of the LWIRO Natural Sciences Research Center (CRSN / LWIRO) took place in the Democratic Republic of Congo in the Kabare Territory. Phytochemical screening has enabled us to observe that saponins, quinones, phenols, lipoids, glycosides, terpenoids, tannoids, flavonoids, and alkaloids are in high concentration while steroids are moderately concentrated in the different parts of the plant. The thin layer chromatography of the saponins shows that the plant contains steroidal and triterpene saponins. The Myrica salicifolia Hochst plant. ex. A. Rich has molluscicidal activity, it can be used in the fight against molluscs Biomphalaria pfeifferi and Lymnaea natalensis intermediate hosts of bilharziasis and fascioliosis. The lethal doses (LD50) of the saponin extracts from the bark of the roots and stems were 0.016 and 0.018 mg / mL respectively for Biomphalaria pfeifferi and 0.022 and 0.021 mg / mL for Lymnaea natalensis.

Published in International Journal of Pharmacy and Chemistry (Volume 6, Issue 3)
DOI 10.11648/j.ijpc.20200603.11
Page(s) 26-30
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Extraction, Identification, Characterization, Molluscicide Activity Test

References
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[2] Zirihi G. N., 2006. Botanical, pharmacological and phytochemical studies of some anti-malarial and / or immunogenic medicinal plants used in the Bété of the Department of Issia, in the west of Coted’Ivoire. State Doctorate Thesis, University of Cocody-Abidjan, UFR Biosciences, 126p.
[3] P. enchew, 2010. Study of the extraction and purification processes of bioactive products from plants by coupling separation techniques at low and high pressures. Doctoral thesis, Institut National Polytechnique de Toulouse Discipline or specialty: Process and Environmental Engineering, 239p.
[4] Kloos et MCullough F. S., 1981. Plant molluscicid: A review, Geneva, document no WHO/VBC/813.834-WHO/SCH/81.59, 33p.
[5] Bagalwa M., Voutquenne-Nazabadioko L., Sayagh C., Bashwira S., 2010. Evaluation of the biological activity of the molluscicidal fraction of Solanum sisymbriifolium against non target organisms, Fitoterapia, 81, 767–771.
[6] Bagalwa M., Chifundera K., 2007. Environnemental impact evaluation of the Stem barks extract of Maesa lanceolata used in Democratic Republic of Congo. Journal of Ehno pharmacology, 114, 281-284.
[7] Maroyi A., 2014. Alternative Medicines for HIV/AIDS: Insight from Traditional Medicines Use in Sub-Saharan Africa. Tropical Journal of Pharmaceutical Research, ISSN: 1596-5996, 9p.
[8] Kirira P. G, Rukunga G. M,, Wanyonyi A. W., Muregi F. M,, Gathirwa J. W,, Muthaura C. N,, Omar S. A,, Tolo F,, Mungai G. M,, Ndiege O., 2011. Anti-plasmodial activity and toxicity of extracts of plants used in traditional malaria therapy in Meru and Kilifi Districts of Kenya.
[9] KNjung'e, Gichuru M., Josphat M. i, Kuria K., 2002. Analgesic and effets antipyrétic of Myrica salicifolia.
[10] Silva B. J. C., Ana M. L., 2015. Recent Breakthroughs in the Antioxidant and Anti-Inflammatory Effects of Morella and Myrica Species, International Journal of Molecular Sciences, ISSN 1422-0067, 20p.
[11] Jhoo J-W., Sang S., He K., Cheng X., Zhu N., Stark RE., Zheng Q. Y., Rosen RT,; Ho CT., 2001. Characterization of the triterpene saponins of the roots and rhizomes of bluecohosh (caulophyllum thalictroides). J. Agric. Food. Chem. 49p.
[12] Vauquenne L., Kokougan C., Pouny I., Litoudon M., 2002. Triterpenoid saponines and acyclated, prosapogenins from Harpullia austro-coledsnica. Phytochemistry, 59p.
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    Faustin Safari Habari, Charles Muhigirwa Balagizi, Bahati Rusimbuka Marcel, Jean Jacques Bagalwa Mashimago. (2020). Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro. International Journal of Pharmacy and Chemistry, 6(3), 26-30. https://doi.org/10.11648/j.ijpc.20200603.11

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    ACS Style

    Faustin Safari Habari; Charles Muhigirwa Balagizi; Bahati Rusimbuka Marcel; Jean Jacques Bagalwa Mashimago. Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro. Int. J. Pharm. Chem. 2020, 6(3), 26-30. doi: 10.11648/j.ijpc.20200603.11

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    AMA Style

    Faustin Safari Habari, Charles Muhigirwa Balagizi, Bahati Rusimbuka Marcel, Jean Jacques Bagalwa Mashimago. Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro. Int J Pharm Chem. 2020;6(3):26-30. doi: 10.11648/j.ijpc.20200603.11

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  • @article{10.11648/j.ijpc.20200603.11,
      author = {Faustin Safari Habari and Charles Muhigirwa Balagizi and Bahati Rusimbuka Marcel and Jean Jacques Bagalwa Mashimago},
      title = {Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {6},
      number = {3},
      pages = {26-30},
      doi = {10.11648/j.ijpc.20200603.11},
      url = {https://doi.org/10.11648/j.ijpc.20200603.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20200603.11},
      abstract = {Our study focused on “Extraction identification and characterization of saponins from the plant Myrica salicifolia Hochst. ex. A. Rich and molluscicidal activity test in vitro". The objective of this study is to extract the natural substances present in the different parts (leaves, bark of the stem and roots); isolate the saponins, characterize them by chromatography and determine the lethal dose of the saponins isolated against aquatic molluscs. After the analyzes, the Malacology laboratory of the LWIRO Natural Sciences Research Center (CRSN / LWIRO) took place in the Democratic Republic of Congo in the Kabare Territory. Phytochemical screening has enabled us to observe that saponins, quinones, phenols, lipoids, glycosides, terpenoids, tannoids, flavonoids, and alkaloids are in high concentration while steroids are moderately concentrated in the different parts of the plant. The thin layer chromatography of the saponins shows that the plant contains steroidal and triterpene saponins. The Myrica salicifolia Hochst plant. ex. A. Rich has molluscicidal activity, it can be used in the fight against molluscs Biomphalaria pfeifferi and Lymnaea natalensis intermediate hosts of bilharziasis and fascioliosis. The lethal doses (LD50) of the saponin extracts from the bark of the roots and stems were 0.016 and 0.018 mg / mL respectively for Biomphalaria pfeifferi and 0.022 and 0.021 mg / mL for Lymnaea natalensis.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Extraction Identification Characterization of Saponins from the Myrica Salicifolia Hochst Plant. ex. A. Rich (Myricaceae) and Molluscicidal Activity Test in Vitro
    AU  - Faustin Safari Habari
    AU  - Charles Muhigirwa Balagizi
    AU  - Bahati Rusimbuka Marcel
    AU  - Jean Jacques Bagalwa Mashimago
    Y1  - 2020/07/17
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijpc.20200603.11
    DO  - 10.11648/j.ijpc.20200603.11
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 26
    EP  - 30
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20200603.11
    AB  - Our study focused on “Extraction identification and characterization of saponins from the plant Myrica salicifolia Hochst. ex. A. Rich and molluscicidal activity test in vitro". The objective of this study is to extract the natural substances present in the different parts (leaves, bark of the stem and roots); isolate the saponins, characterize them by chromatography and determine the lethal dose of the saponins isolated against aquatic molluscs. After the analyzes, the Malacology laboratory of the LWIRO Natural Sciences Research Center (CRSN / LWIRO) took place in the Democratic Republic of Congo in the Kabare Territory. Phytochemical screening has enabled us to observe that saponins, quinones, phenols, lipoids, glycosides, terpenoids, tannoids, flavonoids, and alkaloids are in high concentration while steroids are moderately concentrated in the different parts of the plant. The thin layer chromatography of the saponins shows that the plant contains steroidal and triterpene saponins. The Myrica salicifolia Hochst plant. ex. A. Rich has molluscicidal activity, it can be used in the fight against molluscs Biomphalaria pfeifferi and Lymnaea natalensis intermediate hosts of bilharziasis and fascioliosis. The lethal doses (LD50) of the saponin extracts from the bark of the roots and stems were 0.016 and 0.018 mg / mL respectively for Biomphalaria pfeifferi and 0.022 and 0.021 mg / mL for Lymnaea natalensis.
    VL  - 6
    IS  - 3
    ER  - 

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Author Information
  • Geochemistry and Environment Department, Goma Volcano Observatory, Goma, Democratic Republic of Congo

  • Geochemistry and Environment Department, Goma Volcano Observatory, Goma, Democratic Republic of Congo

  • Geochemistry and Environment Department, Goma Volcano Observatory, Goma, Democratic Republic of Congo

  • Malacology Laboratory, Biology Department, Natural Sciences Research Center, Lwiro, Bukavu, Democratic Republic of Congo

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