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Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex
International Journal of Pharmacy and Chemistry
Volume 6, Issue 5, September 2020, Pages: 60-64
Received: Jul. 22, 2020; Accepted: Aug. 24, 2020; Published: Oct. 26, 2020
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Larysa Lenchyk, Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine
Taras Upyr, Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine
Shahm Mohammed, Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine
Mykola Komisarenko, Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine
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Medicines on the basis of herbs are increasingly used for treating of many diseases and provide the following actions laxative, hepatoprotective, membrane stabilizing and antimicrobial. The plant Prunus domestica L., family Rosaceae is widely cultivated worldwide. The recent studies have shown that fruits have antioxidant, anticancer, antihyperglycemic, anti-hyperlipidemic, antihypertensive, antiosteoporosis, laxative and hepatoprotective activities. As raw material plum of Ukrainian variety ‘vengerka’ was taken for the research and pectin complex from plum fruits was obtained. The yield of the pectin from the fresh plum fruits was 8,6% of a dry weight. The analysis of plum pectin was performed by HPLC and spectrophotometry methods. 16 amino acids were identified and their contents were determined. It was found that plum pectin contained essential amino acids such as threonine, valine, isoleucine, leucine, phenylalanine, and histidine. The total amount of free and bound amino acids was 33.70 μg/mg, while free amino acids – 1.18 μg/mg. The amount of bound amino acids was 32,52 μg/mg. The content of free amino acids was only 3.50% of the amount of all amino acids found in the pectin complex. L-Aspartic and L-Proline predominated among free amino acids. Among the bound amino acids, L-Aspartic and L-Glutamic acids dominated. The content of the total amount of phenolic compounds calculated with reference to gallic acid in the pectin complex was 0.43%, and the content of hydroxycinnamic acids calculated with reference to chlorogenic acid in this substance was 0.09%.
Plum, Fruits, Pectin, Amino Acids, Phenolics, Hydroxycinnamic Acids
To cite this article
Larysa Lenchyk, Taras Upyr, Shahm Mohammed, Mykola Komisarenko, Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex, International Journal of Pharmacy and Chemistry. Special Issue: Drug Research: Current Insights for Search, Development and Analysis. Vol. 6, No. 5, 2020, pp. 60-64. doi: 10.11648/j.ijpc.20200605.12
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Pharmaceutical encyclopedia (2016) 2nd ed.: Chairman Ed. Council Chernykh V. P. Kiev: “MORION”; 1952.
Compendium Medicines: A Handbook. URL:
Upyr T., Mohammed Shahm Basim, Ali Sahlani Bashar Al-Jabbar, Lenchyk L., Senyuk, I., Kyslychenko V. (2018) Phytochemical and pharmacological study of polysaccharide complexes of Prunus Domestica fruit. ScienceRise: Pharmaceutical Science. №3 (13): 3237.
Mohammed SB, Upyr TV, Shapoval OM, Lenchyk LV, Georgiev K. (2019) Determination of phenolic compounds in Prunus domestica fruits extract and its pharmacological activity. J of IMAB. № 25 (2): 2589-2594. DOI:
Lenchyk LV. (2015) Determination of phenolic compounds in Prunus domestica leaves extract. Scripta Scientifica Pharmaceutica № 2 (2): 35-39.
Mohamed Shakhm B., Upyr T. V., Lenchyk L. V., Komisarenko M. A. (2019) Studying of the mineral composition of substances obtained from Prunus domestica fruits. Coliection of scientific works of staff member of P. L. Shupyk NMAPE № 33: 12-18.
Senyuk I., Al-Sahlani Bashar Jabar, Lenchyk L. (2017) Investigation of different substances cathartic properties made from Prunus domestica Ukrainian Biopharmaceutical Journal, № 5 (52): 21-25.
Zagayko A, Senuyk I, Lenchyk L, Galimullin R. (2014) Study of antioxidant activity of the extract from plum ordinary leaves. Ukrainian biopharmaceutical journal. № 1 (30): 25-28.
Al-Sahlanee Bashar Jabbar Ali, Senyuk I. V., Lenchyk L. V., Upyr T. V., (2019) The study of influence of the extracts obtained from Prunus domestica fruits on lipid peroxydation and antioxidant system indicators in liver. Farmatsevtychnyi zhurnal Т. 74, № 6: 85-9.
Senyuk I. V, Al-Sahlanee Bashar Jabbar Ali, Mohammed Shahm Basim (2018) Study of hepatoprotective action of extracts from plum fruit. Pharmaceutical review № 4: 57-61.
The State Pharmacopoeia of Ukraine/State Enterprise “Ukrainian scientific Pharmacopoeial center”. Kharkiv: RІRЕG, 2008. 620 p.
Henderson John W., Ricker Robert D., Bidlingmeyer Brian A., and Woodward Rapid Cliff, (1999). Accurate, Sensitive, and Reproducible HPLC Analysis of Amino Acids Amino Acid Analysis Using Zorbax Eclipse-AAA Columns and the Agilent 1100 HPLC. Agilent Technical Note: 59801193E.
Jámbor A., Molnár-Perl I. (2009) Quantitation of amino acids in plasma by high performance liquid chromatography: Simultaneous deproteinization and derivatization with 9-fluorenylmethyloxycarbonyl chloride. Journal of Chromatography A. 1216: 6218–6223.
Jámbor A., Molnár-Perl I. (2009) Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride. Literature overview and further study. Journal of Chromatography A. № 1216: 3064–3077.
Lenchyk LV. (2016) Study of amino acid composition of extracts obtained from cherry leaves and shoots, leaves of apricot and peach. News of Pharmacy. № 2: 9–11.
Chen LJ, Hrazdina G. (1981) Structural aspects of anthocyanin-flavonoid complex formation and its role in plant color. Phytochemistry № 20 (2): 297-303.
Stacewicz-Sapuntzakis M. (2013) Dried plums and their products: composition and health effects--an updated review. Crit Rev Food Sci Nutr. № 53 (12): 1277-302.
Al-Dhabi N., Arasu M, Park C. Ha, Park S. Un. (2015) An up-to-date review of rutin and its biological and pharmacological activities. EXCLI Journal.№ 14: 59-63.
Qaiser J, Naveed A. (2011) The pharmacological activities of prunes: The dried Plums. J Med Plant Res. № 5 (9): 1508-1511.
Bouayed J, Rammal H, Dicko A, Younos C, Soulimani R. (2007) Chlorogenic acid, a polyphenol from Prunus domestica (Mirabelle), with coupled anxiolytic and antioxidant effects. J Neurol Sci № 15; 262 (1-2): 77-84.
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