International Journal of Pharmacy and Chemistry

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Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves

Received: 03 September 2016    Accepted: 14 April 2017    Published: 06 May 2017
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Abstract

The objective of this study was to determine the phytoconstituents and antimicrobial activities of the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves. The phytochemical analysis was carried out using standard methods. The Agar well diffusion method was adopted in carrying out the antimicrobial screening. The results of the phytochemical analysis indicated the presence of saponins, tannins, steroids, terpenoids, resins and volatile oils in hexane extract; saponins, alkaloids, tannins and balsams in ethanolic extract and tannins, steroids, terpenoids and volatile oils in ethyl acetate extract. The antimicrobial screening indicated that the hexane extract was active on Escherichia coli, Salmonela typhi, Klebsiela pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. The ethanolic extract was active on all the test organisms. The ethyl acetate extract was active on Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. This indicated that the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves can serve as potential antibiotics in ethno medicines.

DOI 10.11648/j.ijpc.20170303.11
Published in International Journal of Pharmacy and Chemistry (Volume 3, Issue 3, May 2017)
Page(s) 29-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Klebsiela Pneumonia, Phytochemical Analysis, Tannins, Agar well Diffusion, Ethno Medicines

References
[1] Nathaniel L. B. and Addison B. (2015). An illustrated flora of the Northern United States, Canada and the British possessions. The plant list. Retrieved 2015.
[2] Alex V. P., Katherine G. M., Jose C. M. S., Carmen M. M. and Lena S. (2011). Spigeliagenuflexa, phytokeys, 6:47-65.
[3] Neuwinger H. D. 2000. African traditional medicine: a dictionary of plant Use and applications. Med pharm. Scientific, Stuttgart, Germany, pp:589.
[4] Olorunfemi C. J., Joseph O. A., Ambrose O. A. and Rowland I. S. A. (2006). Anthelmintic efficacy of extracts of Spigelia anthelmintic of extracts of Spigelia anthelmia Linn on experimental Nippostrongylusbraziliensis in rats, J. Vet. Sc. 7(3): 229-232.
[5] Okwu D. E. (2001). Evaluation of the Chemical Composition of Indigenous Spices and Flavouring Agents. Global J. Appl. Sci. 7(3): 455-459.
[6] Uzama D. (2009). Phytochemical screening and antibacterial activity of Guava (Psidium guajava L.) crude extracts. Bio. Env. Sc. J. Trop. 6(4):139-142.
[7] Sofowora L. A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Harbone. 1993; 55-71.
[8] Esimone C. O., Adiku M. U. and Okonta J. M. (1998). Preliminary Antimicrobial screening of the ethanolic extract from the Lichen Usnea Subfloridans (L.). J. Pharmaceutic. Res. Dev. 3(2):99-102.
[9] Nwaogu L. A., Ujuwundu C. O. and Mgbemena A. I. 2000. Studies on the Nutritional and phytochemical composition of Amaratus hybridus leaves. Bis-Res. 4:28-31.
[10] Evans, W. C. and Trease, G. E. (2002). Pharmacognosy, 15th Ed. London: Saunders publishers. pp 42- 44, 246-249, 304-306, 331-332.
[11] Brain, K. R. and Tuner (1975). The practical evaluation of phytochemistry pharmaceuticals. Wright science chila Bright. pp. 120-140.
[12] Uzama D., Bwai M. D. and Thomas S. A. (2012). The phytochemical, proximate and elemental analyses of Securinega virosa leaf extracts, Research J. in Engg and Applied Sciences. 1(6):351- 354.
[13] Adeyeyi E. I. and Okokiti M. K. O. (1999). Proximate composition andsome nutritional valuable mineral of two varieties of Capsicum annum (Bell and Cherry peppers). Discovery Innovation. 11:75 – 81.
[14] Akindahunsi A. A. and Salawu S. O. (2005). Phytochemical screening and nutrient-antinutrient composition of selected tropical green leafy vegetables. Afr. J. Biotech. 4:497-501.
[15] Antia B. S., Akpan E. J., Okon P. A. and Umoren U. I. (2006). Proximate composition and phytochemical constituents of leaves of some acalypha species. Park J. Nutri. 5:166-168.
Author Information
  • Chemistry Advanced Research Centre, Sheda Science and Technology Complex, Garki, Abuja, Nigeria

  • Department of Forestry and Environmental Management, College of Natural Resources and Environmental Management, Micheal Okpara University of Agriculture, Umudike, Umuahia, Nigeria

  • Chemistry Advanced Research Centre, Sheda Science and Technology Complex, Garki, Abuja, Nigeria

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    Uzama Danlami, Osuagwu Eberechi Cecilia, Osuagwu Magnus Ifeanyi. (2017). Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves. International Journal of Pharmacy and Chemistry, 3(3), 29-32. https://doi.org/10.11648/j.ijpc.20170303.11

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    Uzama Danlami; Osuagwu Eberechi Cecilia; Osuagwu Magnus Ifeanyi. Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves. Int. J. Pharm. Chem. 2017, 3(3), 29-32. doi: 10.11648/j.ijpc.20170303.11

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    AMA Style

    Uzama Danlami, Osuagwu Eberechi Cecilia, Osuagwu Magnus Ifeanyi. Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves. Int J Pharm Chem. 2017;3(3):29-32. doi: 10.11648/j.ijpc.20170303.11

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  • @article{10.11648/j.ijpc.20170303.11,
      author = {Uzama Danlami and Osuagwu Eberechi Cecilia and Osuagwu Magnus Ifeanyi},
      title = {Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {3},
      number = {3},
      pages = {29-32},
      doi = {10.11648/j.ijpc.20170303.11},
      url = {https://doi.org/10.11648/j.ijpc.20170303.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijpc.20170303.11},
      abstract = {The objective of this study was to determine the phytoconstituents and antimicrobial activities of the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves. The phytochemical analysis was carried out using standard methods. The Agar well diffusion method was adopted in carrying out the antimicrobial screening. The results of the phytochemical analysis indicated the presence of saponins, tannins, steroids, terpenoids, resins and volatile oils in hexane extract; saponins, alkaloids, tannins and balsams in ethanolic extract and tannins, steroids, terpenoids and volatile oils in ethyl acetate extract. The antimicrobial screening indicated that the hexane extract was active on Escherichia coli, Salmonela typhi, Klebsiela pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. The ethanolic extract was active on all the test organisms. The ethyl acetate extract was active on Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. This indicated that the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves can serve as potential antibiotics in ethno medicines.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of the Phytochemicals and Antimicrobial Activities of the Ethanolic, Hexane and Ethyl Acetate Extracts of Spigelia Anthelmia Leaves
    AU  - Uzama Danlami
    AU  - Osuagwu Eberechi Cecilia
    AU  - Osuagwu Magnus Ifeanyi
    Y1  - 2017/05/06
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijpc.20170303.11
    DO  - 10.11648/j.ijpc.20170303.11
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 29
    EP  - 32
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20170303.11
    AB  - The objective of this study was to determine the phytoconstituents and antimicrobial activities of the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves. The phytochemical analysis was carried out using standard methods. The Agar well diffusion method was adopted in carrying out the antimicrobial screening. The results of the phytochemical analysis indicated the presence of saponins, tannins, steroids, terpenoids, resins and volatile oils in hexane extract; saponins, alkaloids, tannins and balsams in ethanolic extract and tannins, steroids, terpenoids and volatile oils in ethyl acetate extract. The antimicrobial screening indicated that the hexane extract was active on Escherichia coli, Salmonela typhi, Klebsiela pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. The ethanolic extract was active on all the test organisms. The ethyl acetate extract was active on Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Pseudomonas ariginosa but inactive on staphylococcus aureus. This indicated that the hexane, ethanolic and ethyl acetate extracts of Spigelia anthelmia leaves can serve as potential antibiotics in ethno medicines.
    VL  - 3
    IS  - 3
    ER  - 

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