First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered.
| Published in | International Journal of Pharmacy and Chemistry (Volume 2, Issue 2) |
| DOI | 10.11648/j.ijpc.20160202.15 |
| Page(s) | 31-38 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Anthraquinones, Alkaloids, Fluorescent Drugs, Laser-Scanning Confocal Microscopy, Luminescence Microscopy, Secretory Cells, Sesquiterpene Lactones, Terpenes
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APA Style
Victoria V. Roshchina, Andrei V. Kuchin, Valerii A. Yashin. (2016). Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy. International Journal of Pharmacy and Chemistry, 2(2), 31-38. https://doi.org/10.11648/j.ijpc.20160202.15
ACS Style
Victoria V. Roshchina; Andrei V. Kuchin; Valerii A. Yashin. Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy. Int. J. Pharm. Chem. 2016, 2(2), 31-38. doi: 10.11648/j.ijpc.20160202.15
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Download@article{10.11648/j.ijpc.20160202.15,
author = {Victoria V. Roshchina and Andrei V. Kuchin and Valerii A. Yashin},
title = {Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy},
journal = {International Journal of Pharmacy and Chemistry},
volume = {2},
number = {2},
pages = {31-38},
doi = {10.11648/j.ijpc.20160202.15},
url = {https://doi.org/10.11648/j.ijpc.20160202.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20160202.15},
abstract = {First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered.},
year = {2016}
}
TY - JOUR T1 - Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy AU - Victoria V. Roshchina AU - Andrei V. Kuchin AU - Valerii A. Yashin Y1 - 2016/11/03 PY - 2016 N1 - https://doi.org/10.11648/j.ijpc.20160202.15 DO - 10.11648/j.ijpc.20160202.15 T2 - International Journal of Pharmacy and Chemistry JF - International Journal of Pharmacy and Chemistry JO - International Journal of Pharmacy and Chemistry SP - 31 EP - 38 PB - Science Publishing Group SN - 2575-5749 UR - https://doi.org/10.11648/j.ijpc.20160202.15 AB - First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered. VL - 2 IS - 2 ER -
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