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Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex

Received: 22 July 2020    Accepted: 24 August 2020    Published: 26 October 2020
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Abstract

Medicines on the basis of herbs are increasingly used for treating of many diseases and provide the following actions laxative, hepatoprotective, membrane stabilizing and antimicrobial. The plant Prunus domestica L., family Rosaceae is widely cultivated worldwide. The recent studies have shown that fruits have antioxidant, anticancer, antihyperglycemic, anti-hyperlipidemic, antihypertensive, antiosteoporosis, laxative and hepatoprotective activities. As raw material plum of Ukrainian variety ‘vengerka’ was taken for the research and pectin complex from plum fruits was obtained. The yield of the pectin from the fresh plum fruits was 8,6% of a dry weight. The analysis of plum pectin was performed by HPLC and spectrophotometry methods. 16 amino acids were identified and their contents were determined. It was found that plum pectin contained essential amino acids such as threonine, valine, isoleucine, leucine, phenylalanine, and histidine. The total amount of free and bound amino acids was 33.70 μg/mg, while free amino acids – 1.18 μg/mg. The amount of bound amino acids was 32,52 μg/mg. The content of free amino acids was only 3.50% of the amount of all amino acids found in the pectin complex. L-Aspartic and L-Proline predominated among free amino acids. Among the bound amino acids, L-Aspartic and L-Glutamic acids dominated. The content of the total amount of phenolic compounds calculated with reference to gallic acid in the pectin complex was 0.43%, and the content of hydroxycinnamic acids calculated with reference to chlorogenic acid in this substance was 0.09%.

Published in International Journal of Pharmacy and Chemistry (Volume 6, Issue 5)

This article belongs to the Special Issue Drug Research: Current Insights for Search, Development and Analysis

DOI 10.11648/j.ijpc.20200605.12
Page(s) 60-64
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Plum, Fruits, Pectin, Amino Acids, Phenolics, Hydroxycinnamic Acids

References
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[2] Compendium Medicines: A Handbook. URL: https://compendium.com.ua.
[3] Upyr T., Mohammed Shahm Basim, Ali Sahlani Bashar Al-Jabbar, Lenchyk L., Senyuk, I., Kyslychenko V. (2018) Phytochemical and pharmacological study of polysaccharide complexes of Prunus Domestica fruit. ScienceRise: Pharmaceutical Science. №3 (13): 3237.
[4] Mohammed SB, Upyr TV, Shapoval OM, Lenchyk LV, Georgiev K. (2019) Determination of phenolic compounds in Prunus domestica fruits extract and its pharmacological activity. J of IMAB. № 25 (2): 2589-2594. DOI: https://doi.org/10.5272/jimab.2019252.2589.
[5] Lenchyk LV. (2015) Determination of phenolic compounds in Prunus domestica leaves extract. Scripta Scientifica Pharmaceutica № 2 (2): 35-39.
[6] Mohamed Shakhm B., Upyr T. V., Lenchyk L. V., Komisarenko M. A. (2019) Studying of the mineral composition of substances obtained from Prunus domestica fruits. Coliection of scientific works of staff member of P. L. Shupyk NMAPE № 33: 12-18. http://nbuv.gov.ua/UJRN/Znpsnmapo_2019_33_4.
[7] Senyuk I., Al-Sahlani Bashar Jabar, Lenchyk L. (2017) Investigation of different substances cathartic properties made from Prunus domestica Ukrainian Biopharmaceutical Journal, № 5 (52): 21-25.
[8] Zagayko A, Senuyk I, Lenchyk L, Galimullin R. (2014) Study of antioxidant activity of the extract from plum ordinary leaves. Ukrainian biopharmaceutical journal. № 1 (30): 25-28.
[9] Al-Sahlanee Bashar Jabbar Ali, Senyuk I. V., Lenchyk L. V., Upyr T. V., (2019) The study of influence of the extracts obtained from Prunus domestica fruits on lipid peroxydation and antioxidant system indicators in liver. Farmatsevtychnyi zhurnal Т. 74, № 6: 85-9.
[10] Senyuk I. V, Al-Sahlanee Bashar Jabbar Ali, Mohammed Shahm Basim (2018) Study of hepatoprotective action of extracts from plum fruit. Pharmaceutical review № 4: 57-61.
[11] The State Pharmacopoeia of Ukraine/State Enterprise “Ukrainian scientific Pharmacopoeial center”. Kharkiv: RІRЕG, 2008. 620 p.
[12] Henderson John W., Ricker Robert D., Bidlingmeyer Brian A., and Woodward Rapid Cliff, (1999). Accurate, Sensitive, and Reproducible HPLC Analysis of Amino Acids Amino Acid Analysis Using Zorbax Eclipse-AAA Columns and the Agilent 1100 HPLC. Agilent Technical Note: 59801193E. https://www.agilent.com/cs/library/chromatograms/59801193.pdf.
[13] Jámbor A., Molnár-Perl I. (2009) Quantitation of amino acids in plasma by high performance liquid chromatography: Simultaneous deproteinization and derivatization with 9-fluorenylmethyloxycarbonyl chloride. Journal of Chromatography A. 1216: 6218–6223.
[14] Jámbor A., Molnár-Perl I. (2009) Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride. Literature overview and further study. Journal of Chromatography A. № 1216: 3064–3077.
[15] Lenchyk LV. (2016) Study of amino acid composition of extracts obtained from cherry leaves and shoots, leaves of apricot and peach. News of Pharmacy. № 2: 9–11.
[16] Chen LJ, Hrazdina G. (1981) Structural aspects of anthocyanin-flavonoid complex formation and its role in plant color. Phytochemistry № 20 (2): 297-303.
[17] Stacewicz-Sapuntzakis M. (2013) Dried plums and their products: composition and health effects--an updated review. Crit Rev Food Sci Nutr. № 53 (12): 1277-302.
[18] Al-Dhabi N., Arasu M, Park C. Ha, Park S. Un. (2015) An up-to-date review of rutin and its biological and pharmacological activities. EXCLI Journal.№ 14: 59-63.
[19] Qaiser J, Naveed A. (2011) The pharmacological activities of prunes: The dried Plums. J Med Plant Res. № 5 (9): 1508-1511.
[20] Bouayed J, Rammal H, Dicko A, Younos C, Soulimani R. (2007) Chlorogenic acid, a polyphenol from Prunus domestica (Mirabelle), with coupled anxiolytic and antioxidant effects. J Neurol Sci № 15; 262 (1-2): 77-84.
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  • APA Style

    Larysa Lenchyk, Taras Upyr, Shahm Mohammed, Mykola Komisarenko. (2020). Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex. International Journal of Pharmacy and Chemistry, 6(5), 60-64. https://doi.org/10.11648/j.ijpc.20200605.12

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    ACS Style

    Larysa Lenchyk; Taras Upyr; Shahm Mohammed; Mykola Komisarenko. Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex. Int. J. Pharm. Chem. 2020, 6(5), 60-64. doi: 10.11648/j.ijpc.20200605.12

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    AMA Style

    Larysa Lenchyk, Taras Upyr, Shahm Mohammed, Mykola Komisarenko. Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex. Int J Pharm Chem. 2020;6(5):60-64. doi: 10.11648/j.ijpc.20200605.12

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  • @article{10.11648/j.ijpc.20200605.12,
      author = {Larysa Lenchyk and Taras Upyr and Shahm Mohammed and Mykola Komisarenko},
      title = {Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {6},
      number = {5},
      pages = {60-64},
      doi = {10.11648/j.ijpc.20200605.12},
      url = {https://doi.org/10.11648/j.ijpc.20200605.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20200605.12},
      abstract = {Medicines on the basis of herbs are increasingly used for treating of many diseases and provide the following actions laxative, hepatoprotective, membrane stabilizing and antimicrobial. The plant Prunus domestica L., family Rosaceae is widely cultivated worldwide. The recent studies have shown that fruits have antioxidant, anticancer, antihyperglycemic, anti-hyperlipidemic, antihypertensive, antiosteoporosis, laxative and hepatoprotective activities. As raw material plum of Ukrainian variety ‘vengerka’ was taken for the research and pectin complex from plum fruits was obtained. The yield of the pectin from the fresh plum fruits was 8,6% of a dry weight. The analysis of plum pectin was performed by HPLC and spectrophotometry methods. 16 amino acids were identified and their contents were determined. It was found that plum pectin contained essential amino acids such as threonine, valine, isoleucine, leucine, phenylalanine, and histidine. The total amount of free and bound amino acids was 33.70 μg/mg, while free amino acids – 1.18 μg/mg. The amount of bound amino acids was 32,52 μg/mg. The content of free amino acids was only 3.50% of the amount of all amino acids found in the pectin complex. L-Aspartic and L-Proline predominated among free amino acids. Among the bound amino acids, L-Aspartic and L-Glutamic acids dominated. The content of the total amount of phenolic compounds calculated with reference to gallic acid in the pectin complex was 0.43%, and the content of hydroxycinnamic acids calculated with reference to chlorogenic acid in this substance was 0.09%.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Study of Amino Acid Composition of Prunus Domestica Fruits Pectin Complex
    AU  - Larysa Lenchyk
    AU  - Taras Upyr
    AU  - Shahm Mohammed
    AU  - Mykola Komisarenko
    Y1  - 2020/10/26
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijpc.20200605.12
    DO  - 10.11648/j.ijpc.20200605.12
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 60
    EP  - 64
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20200605.12
    AB  - Medicines on the basis of herbs are increasingly used for treating of many diseases and provide the following actions laxative, hepatoprotective, membrane stabilizing and antimicrobial. The plant Prunus domestica L., family Rosaceae is widely cultivated worldwide. The recent studies have shown that fruits have antioxidant, anticancer, antihyperglycemic, anti-hyperlipidemic, antihypertensive, antiosteoporosis, laxative and hepatoprotective activities. As raw material plum of Ukrainian variety ‘vengerka’ was taken for the research and pectin complex from plum fruits was obtained. The yield of the pectin from the fresh plum fruits was 8,6% of a dry weight. The analysis of plum pectin was performed by HPLC and spectrophotometry methods. 16 amino acids were identified and their contents were determined. It was found that plum pectin contained essential amino acids such as threonine, valine, isoleucine, leucine, phenylalanine, and histidine. The total amount of free and bound amino acids was 33.70 μg/mg, while free amino acids – 1.18 μg/mg. The amount of bound amino acids was 32,52 μg/mg. The content of free amino acids was only 3.50% of the amount of all amino acids found in the pectin complex. L-Aspartic and L-Proline predominated among free amino acids. Among the bound amino acids, L-Aspartic and L-Glutamic acids dominated. The content of the total amount of phenolic compounds calculated with reference to gallic acid in the pectin complex was 0.43%, and the content of hydroxycinnamic acids calculated with reference to chlorogenic acid in this substance was 0.09%.
    VL  - 6
    IS  - 5
    ER  - 

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Author Information
  • Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine

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