Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model

Mohammad Rezaeisadrabadi; Mohammad Hossein Dashti-Rahmatabadi; Shamin Ghobadi; Fatemeh Haddad; Shokouh Taghipour Zahir; Mansour Ahmadi; Mohammad Reza Lotfaliani; Aghdas Mirjalili; Ali Rezaei

doi:doi:10.11648/j.ijcems.20150103.15

Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model

Mohammad Rezaeisadrabadi, Mohammad Hossein Dashti-Rahmatabadi, Shamin Ghobadi, Fatemeh Haddad, Shokouh Taghipour Zahir, Mansour Ahmadi, Mohammad Reza Lotfaliani, Aghdas Mirjalili, Ali Rezaei

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 1, Issue 3)

Received: 12 August 2015 Accepted: 8 September 2015 Published: 9 September 2015

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Abstract

Background. Dexamethasone is a type of steroid medication which enhances the rate of pericyte differentiation and mineralization in vitro with concomitant suppression of calcification inhibitory molecule matrix Gla-protein (MGP). Vitamin K is an essential cofactor in the carboxylation of glutamate residues in a small group of proteins, including MGP. This study tries to assess the efficacy of vitamin K1 on dexamethasone-induced media elastocalcinosis in aorta artery and heart muscle in a rat model. Materials and Methods. 110 male rats with a normal weight range of 270 ± 20 were enrolled in this study. They received a calcification-inducing diet containing both vitamin K1 and dexamethasone during 6 or 12 weeks and were randomly assigned into two groups; a basic group (n=30), and an experimental group (n=80). The experimental group was divided into two groups receiving treatment during 6 and 12 weeks. Administration of dexamethasone was 0.5 mg/kg, intraperitoneal (IP). Vitamin K intakes were different including 5, 10, and 20 µg/kg, which were considered as low, moderate, and high intake, respectively. Results. Plasma concentrations of calcium were not affected by the different regimes and ranged between 2.27 and 2.31 millimolar (mM) (mean ± SD: 2.29 ± 0.02). According to the findings of pathologic biopsy of aorta artery and heart muscle, treatment of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. Conclusion. Administration of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. On the other hand, structure and histology of vessels did not change following intake of vitamin K1, therefore, different dosages of vitamin K could not affect the aorta artery status.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 1, Issue 3)
DOI	10.11648/j.ijcems.20150103.15
Page(s)	60-64
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Media Elastocalcinosis, Dexamethasone, Vitamin K, Aorta Artery, Heart Muscle

References

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[2]	Vermer C, Braam L. Role of K vitamins in the regulation of tissue calcification. J Bone Miner Metab. 2001;19:201-206.
[3]	Wallin R, Schurgers L, Wajih N. Effects of the blood coagulation vitamin K as an inhibitor of arterial calcification. Thrombosis Research. 2008;122:411-417.
[4]	Spronk HM, Soute BA, Schurgers LJ, Thijssen HH, De Mey JG, Vermeer C. Tissue-specific utilization of menaquinone-4 results in the prevention of arterial calcification in warfarin-treated rats. J Vasc Res. 2003;40:531–537.
[5]	Thane CW, Paul AA, Bates CJ, Bolton-Smith C, Prentice A, Shearer MJ. Intake and sources of phylloquinone (vitamin K1): variation with socio-demographic and lifestyle factors in a national sample of British elderly people. Br J Nutr. 2002;87:605-613.
[6]	McKeown NM, Jacques PF, Gundberg CM, et al. Dietary and nondietary determinants of vitamin K biochemical measures in men and women. J Nutr. 2002;132:1329-1334.
[7]	Shanahan CM, Weissberg PL, Metcalfe JC. Isolation of gene markers of differentiated and proliferating vascular smooth muscle cells. Circ Res. 1993;73:193–204.
[8]	Davidson RT, Foley AL, Engelke JA, Suttie JW. Conversion of dietary phylloquinone to tissue menaquinone-4 in rats is not dependent on gut bacteria. J Nutr. 1998;128:220-223.
[9]	Willenberg T, Diehm N, Zwahlen M, et al. Impact of long-term corticosteroid therapy on the distribution pattern of lower limb atherosclerosis. Eur J Vasc Endovasc Surg. 2010;39:441-446.
[10]	Nashel DJ. Is atherosclerosis a complication of long-term corticosteroid treatment? Am J Med. 1986;80:925-929.
[11]	Miyahara T, Nemoto M, Tukamoto S, et al. Induction of metallothionein and stimulation of calcification by dexamethasone in cultured clonal osteogenic cells, MC3T3-E1. Toxicol Lett. 1991;57:257-267.
[12]	Kirton JP, Wilkinson FL, Canfield AE, Alexander MY. Dexamethasone downregulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification. Circ Res. 2006;98:1264-1272.
[13]	Maas AH, van der Schouw YT, Beijerinck D, et al. Vitamin K intake and calcifications in breast arteries. Maturitas. 2007;56:273-279.
[14]	Rees K, Guraewal S, Wong YL, et al. Is vitamin K consumption associated with cardio-metabolic disorders? A systematic review. Maturitas. 2010; 67:121-128.

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Mohammad Rezaeisadrabadi, Mohammad Hossein Dashti-Rahmatabadi, Shamin Ghobadi, Fatemeh Haddad, Shokouh Taghipour Zahir, et al. (2015). Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. International Journal of Clinical and Experimental Medical Sciences, 1(3), 60-64. https://doi.org/10.11648/j.ijcems.20150103.15

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ACS Style

Mohammad Rezaeisadrabadi; Mohammad Hossein Dashti-Rahmatabadi; Shamin Ghobadi; Fatemeh Haddad; Shokouh Taghipour Zahir, et al. Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. Int. J. Clin. Exp. Med. Sci. 2015, 1(3), 60-64. doi: 10.11648/j.ijcems.20150103.15

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AMA Style

Mohammad Rezaeisadrabadi, Mohammad Hossein Dashti-Rahmatabadi, Shamin Ghobadi, Fatemeh Haddad, Shokouh Taghipour Zahir, et al. Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. Int J Clin Exp Med Sci. 2015;1(3):60-64. doi: 10.11648/j.ijcems.20150103.15

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@article{10.11648/j.ijcems.20150103.15,
  author = {Mohammad Rezaeisadrabadi and Mohammad Hossein Dashti-Rahmatabadi and Shamin Ghobadi and Fatemeh Haddad and Shokouh Taghipour Zahir and Mansour Ahmadi and Mohammad Reza Lotfaliani and Aghdas Mirjalili and Ali Rezaei},
  title = {Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {1},
  number = {3},
  pages = {60-64},
  doi = {10.11648/j.ijcems.20150103.15},
  url = {https://doi.org/10.11648/j.ijcems.20150103.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20150103.15},
  abstract = {Background. Dexamethasone is a type of steroid medication which enhances the rate of pericyte differentiation and mineralization in vitro with concomitant suppression of calcification inhibitory molecule matrix Gla-protein (MGP). Vitamin K is an essential cofactor in the carboxylation of glutamate residues in a small group of proteins, including MGP. This study tries to assess the efficacy of vitamin K1 on dexamethasone-induced media elastocalcinosis in aorta artery and heart muscle in a rat model. Materials and Methods. 110 male rats with a normal weight range of 270 ± 20 were enrolled in this study. They received a calcification-inducing diet containing both vitamin K1 and dexamethasone during 6 or 12 weeks and were randomly assigned into two groups; a basic group (n=30), and an experimental group (n=80). The experimental group was divided into two groups receiving treatment during 6 and 12 weeks. Administration of dexamethasone was 0.5 mg/kg, intraperitoneal (IP). Vitamin K intakes were different including 5, 10, and 20 µg/kg, which were considered as low, moderate, and high intake, respectively. Results. Plasma concentrations of calcium were not affected by the different regimes and ranged between 2.27 and 2.31 millimolar (mM) (mean ± SD: 2.29 ± 0.02). According to the findings of pathologic biopsy of aorta artery and heart muscle, treatment of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. Conclusion. Administration of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. On the other hand, structure and histology of vessels did not change following intake of vitamin K1, therefore, different dosages of vitamin K could not affect the aorta artery status.},
 year = {2015}
}

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TY - JOUR
T1 - Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model
AU - Mohammad Rezaeisadrabadi
AU - Mohammad Hossein Dashti-Rahmatabadi
AU - Shamin Ghobadi
AU - Fatemeh Haddad
AU - Shokouh Taghipour Zahir
AU - Mansour Ahmadi
AU - Mohammad Reza Lotfaliani
AU - Aghdas Mirjalili
AU - Ali Rezaei
Y1 - 2015/09/09
PY - 2015
N1 - https://doi.org/10.11648/j.ijcems.20150103.15
DO - 10.11648/j.ijcems.20150103.15
T2 - International Journal of Clinical and Experimental Medical Sciences
JF - International Journal of Clinical and Experimental Medical Sciences
JO - International Journal of Clinical and Experimental Medical Sciences
SP - 60
EP - 64
PB - Science Publishing Group
SN - 2469-8032
UR - https://doi.org/10.11648/j.ijcems.20150103.15
AB - Background. Dexamethasone is a type of steroid medication which enhances the rate of pericyte differentiation and mineralization in vitro with concomitant suppression of calcification inhibitory molecule matrix Gla-protein (MGP). Vitamin K is an essential cofactor in the carboxylation of glutamate residues in a small group of proteins, including MGP. This study tries to assess the efficacy of vitamin K1 on dexamethasone-induced media elastocalcinosis in aorta artery and heart muscle in a rat model. Materials and Methods. 110 male rats with a normal weight range of 270 ± 20 were enrolled in this study. They received a calcification-inducing diet containing both vitamin K1 and dexamethasone during 6 or 12 weeks and were randomly assigned into two groups; a basic group (n=30), and an experimental group (n=80). The experimental group was divided into two groups receiving treatment during 6 and 12 weeks. Administration of dexamethasone was 0.5 mg/kg, intraperitoneal (IP). Vitamin K intakes were different including 5, 10, and 20 µg/kg, which were considered as low, moderate, and high intake, respectively. Results. Plasma concentrations of calcium were not affected by the different regimes and ranged between 2.27 and 2.31 millimolar (mM) (mean ± SD: 2.29 ± 0.02). According to the findings of pathologic biopsy of aorta artery and heart muscle, treatment of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. Conclusion. Administration of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. On the other hand, structure and histology of vessels did not change following intake of vitamin K1, therefore, different dosages of vitamin K could not affect the aorta artery status.
VL - 1
IS - 3
ER -

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Author Information

Mohammad Rezaeisadrabadi

Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mohammad Hossein Dashti-Rahmatabadi

Department of Physiology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Shamin Ghobadi

Shahrekord University of Medical Sciences, Shahrekord, Iran
Fatemeh Haddad

Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Shokouh Taghipour Zahir

Department of Pathology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mansour Ahmadi

Department of Physiology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mohammad Reza Lotfaliani

Department of Food and Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Aghdas Mirjalili

Department of Histopathology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Ali Rezaei

Shahrekord University of Medical Sciences, Shahrekord, Iran

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APA Style

Mohammad Rezaeisadrabadi, Mohammad Hossein Dashti-Rahmatabadi, Shamin Ghobadi, Fatemeh Haddad, Shokouh Taghipour Zahir, et al. (2015). Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. International Journal of Clinical and Experimental Medical Sciences, 1(3), 60-64. https://doi.org/10.11648/j.ijcems.20150103.15

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ACS Style

Mohammad Rezaeisadrabadi; Mohammad Hossein Dashti-Rahmatabadi; Shamin Ghobadi; Fatemeh Haddad; Shokouh Taghipour Zahir, et al. Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. Int. J. Clin. Exp. Med. Sci. 2015, 1(3), 60-64. doi: 10.11648/j.ijcems.20150103.15

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AMA Style

Mohammad Rezaeisadrabadi, Mohammad Hossein Dashti-Rahmatabadi, Shamin Ghobadi, Fatemeh Haddad, Shokouh Taghipour Zahir, et al. Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model. Int J Clin Exp Med Sci. 2015;1(3):60-64. doi: 10.11648/j.ijcems.20150103.15

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@article{10.11648/j.ijcems.20150103.15,
  author = {Mohammad Rezaeisadrabadi and Mohammad Hossein Dashti-Rahmatabadi and Shamin Ghobadi and Fatemeh Haddad and Shokouh Taghipour Zahir and Mansour Ahmadi and Mohammad Reza Lotfaliani and Aghdas Mirjalili and Ali Rezaei},
  title = {Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {1},
  number = {3},
  pages = {60-64},
  doi = {10.11648/j.ijcems.20150103.15},
  url = {https://doi.org/10.11648/j.ijcems.20150103.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20150103.15},
  abstract = {Background. Dexamethasone is a type of steroid medication which enhances the rate of pericyte differentiation and mineralization in vitro with concomitant suppression of calcification inhibitory molecule matrix Gla-protein (MGP). Vitamin K is an essential cofactor in the carboxylation of glutamate residues in a small group of proteins, including MGP. This study tries to assess the efficacy of vitamin K1 on dexamethasone-induced media elastocalcinosis in aorta artery and heart muscle in a rat model. Materials and Methods. 110 male rats with a normal weight range of 270 ± 20 were enrolled in this study. They received a calcification-inducing diet containing both vitamin K1 and dexamethasone during 6 or 12 weeks and were randomly assigned into two groups; a basic group (n=30), and an experimental group (n=80). The experimental group was divided into two groups receiving treatment during 6 and 12 weeks. Administration of dexamethasone was 0.5 mg/kg, intraperitoneal (IP). Vitamin K intakes were different including 5, 10, and 20 µg/kg, which were considered as low, moderate, and high intake, respectively. Results. Plasma concentrations of calcium were not affected by the different regimes and ranged between 2.27 and 2.31 millimolar (mM) (mean ± SD: 2.29 ± 0.02). According to the findings of pathologic biopsy of aorta artery and heart muscle, treatment of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. Conclusion. Administration of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. On the other hand, structure and histology of vessels did not change following intake of vitamin K1, therefore, different dosages of vitamin K could not affect the aorta artery status.},
 year = {2015}
}

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