International Journal of Clinical and Experimental Medical Sciences

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A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis

Received: 06 September 2018    Accepted: 20 September 2018    Published: 12 October 2018
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Abstract

Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.

DOI 10.11648/j.ijcems.20180404.12
Published in International Journal of Clinical and Experimental Medical Sciences (Volume 4, Issue 4, July 2018)
Page(s) 63-67
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Inflammatory Bowel Disease, Tumorigenesis, Mice Model

References
[1] Endo K, Shiga H, Kinouchi Y, Shimosegawa T. Inflammatory bowel disease: IBD. Rinsho Byori, 2009, 57(6): 527-532.
[2] Soderlund S, Brandt L, Lapidus A, Karlen P, Brostrom O, Lofberg R, et al. Decreasing time-trends of colorectal cancer in a large cohort of patients with inflammatory bowel disease. Gastroenterology, 2009, 136(5): 1561-1567, 1818-1819.
[3] Adami H O, Bretthauer M, Emilsson L, Hernan M A, Kalager M, Ludvigsson J F, et al. The continuing uncertainty about cancer risk in inflammatory bowel disease. Gut, 2016, 65(6): 889-893.
[4] Itzkowitz S H, Harpaz N. Diagnosis and management of dysplasia in patients with inflammatory bowel diseases. Gastroenterology, 2004, 126(6): 1634-1648.
[5] Vagefi P A, Longo W E. Colorectal cancer in patients with inflammatory bowel disease. Clin Colorectal Cancer, 2005, 4(5): 313-319.
[6] Herszenyi L, Miheller P, Tulassay Z. Carcinogenesis in inflammatory bowel disease. Dig Dis, 2007, 25(3): 267-269.
[7] Eaden J A, Abrams K R, Mayberry J F. The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut, 2001, 48(4): 526-535.
[8] Jess T, Gamborg M, Matzen P, Munkholm P, Sorensen T I. Increased risk of intestinal cancer in Crohn's disease: a meta-analysis of population-based cohort studies. Am J Gastroenterol, 2005, 100(12): 2724-2729.
[9] Lakatos L, Mester G, Erdelyi Z, David G, Pandur T, Balogh M, et al. Risk factors for ulcerative colitis-associated colorectal cancer in a Hungarian cohort of patients with ulcerative colitis: results of a population-based study. Inflamm Bowel Dis, 2006, 12(3): 205-211.
[10] Loftus E J. Epidemiology and risk factors for colorectal dysplasia and cancer in ulcerative colitis. Gastroenterol Clin North Am, 2006, 35(3): 517-531.
[11] Grivennikov S I, Greten F R, Karin M. Immunity, inflammation, and cancer. Cell, 2010, 140(6): 883-899.
[12] Fitzpatrick F A. Inflammation, carcinogenesis and cancer. Int Immunopharmacol, 2001, 1(9-10): 1651-1667.
[13] Ullman T A, Itzkowitz S H. Intestinal inflammation and cancer. Gastroenterology, 2011, 140(6): 1807-1816.
[14] Baker A M, Cross W, Curtius K, Al B I, Choi C R, Davis H L, et al. Evolutionary history of human colitis-associated colorectal cancer. Gut, 2018.
[15] Liu Y, Lu Y, Zhou M, Zhang C, Li X. Construction of colorectal cancer cell line stably expressing mir-101 and identification of the target gene of mir-101. Nan Fang Yi Ke Da Xue Xue Bao, 2014, 34(7): 928-933.
[16] Mathieu A A, Ohl-Seguy E, Dubois M L, Jean D, Jones C, Boudreau F, et al. Subcellular proteomics analysis of different stages of colorectal cancer cell lines. Proteomics, 2016, 16(23): 3009-3018.
[17] Boivin G P, Washington K, Yang K, Ward J M, Pretlow T P, Russell R, et al. Pathology of mouse models of intestinal cancer: consensus report and recommendations. Gastroenterology, 2003, 124(3): 762-777.
[18] Day C P, Merlino G, Van Dyke T. Preclinical mouse cancer models: a maze of opportunities and challenges. Cell, 2015, 163(1): 39-53.
[19] Dotti I, Salas A. Potential Use of Human Stem Cell-Derived Intestinal Organoids to Study Inflammatory Bowel Diseases. Inflamm Bowel Dis, 2018.
[20] Manicassamy S, Manoharan I. Mouse models of acute and chronic colitis. Methods Mol Biol, 2014, 1194: 437-448.
[21] Ostanin D V, Bao J, Koboziev I, Gray L, Robinson-Jackson S A, Kosloski-Davidson M, et al. T cell transfer model of chronic colitis: concepts, considerations, and tricks of the trade. Am J Physiol Gastrointest Liver Physiol, 2009, 296(2): G135-G146.
Author Information
  • Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

  • Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

  • Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

  • Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

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  • APA Style

    Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. (2018). A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. International Journal of Clinical and Experimental Medical Sciences, 4(4), 63-67. https://doi.org/10.11648/j.ijcems.20180404.12

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    ACS Style

    Zexian Chen; Yongle Chen; Xiaowen He; Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int. J. Clin. Exp. Med. Sci. 2018, 4(4), 63-67. doi: 10.11648/j.ijcems.20180404.12

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    AMA Style

    Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan. A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis. Int J Clin Exp Med Sci. 2018;4(4):63-67. doi: 10.11648/j.ijcems.20180404.12

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  • @article{10.11648/j.ijcems.20180404.12,
      author = {Zexian Chen and Yongle Chen and Xiaowen He and Ping Lan},
      title = {A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis},
      journal = {International Journal of Clinical and Experimental Medical Sciences},
      volume = {4},
      number = {4},
      pages = {63-67},
      doi = {10.11648/j.ijcems.20180404.12},
      url = {https://doi.org/10.11648/j.ijcems.20180404.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijcems.20180404.12},
      abstract = {Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis
    AU  - Zexian Chen
    AU  - Yongle Chen
    AU  - Xiaowen He
    AU  - Ping Lan
    Y1  - 2018/10/12
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijcems.20180404.12
    DO  - 10.11648/j.ijcems.20180404.12
    T2  - International Journal of Clinical and Experimental Medical Sciences
    JF  - International Journal of Clinical and Experimental Medical Sciences
    JO  - International Journal of Clinical and Experimental Medical Sciences
    SP  - 63
    EP  - 67
    PB  - Science Publishing Group
    SN  - 2469-8032
    UR  - https://doi.org/10.11648/j.ijcems.20180404.12
    AB  - Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.
    VL  - 4
    IS  - 4
    ER  - 

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