HPLC Determination of Fructo-Oligosaccharides in Dairy Products
International Journal of Food Engineering and Technology
Volume 2, Issue 2, December 2018, Pages: 36-40
Received: Nov. 7, 2018; Accepted: Dec. 4, 2018; Published: Jan. 11, 2019
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Authors
Zhen Zhao, Quality Management Department, Inner Mongolia Yili Industrial Group Co. Ltd, Hohhot, China
Guoyan Wen, Quality Management Department, Inner Mongolia Yili Industrial Group Co. Ltd, Hohhot, China
Cuizhi Li, Quality Management Department, Inner Mongolia Yili Industrial Group Co. Ltd, Hohhot, China
Peng Wan, Quality Management Department, Inner Mongolia Yili Industrial Group Co. Ltd, Hohhot, China
Zhiyong Lv, Quality Management Department, Inner Mongolia Yili Industrial Group Co. Ltd, Hohhot, China
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Abstract
Fructo-oligosaccharides is a natural active substance with excellent physiological functions such as improving the gastrointestinal tract, regulating intestinal flora, etc. It is an important nutrient in infant dairy products. Therefore, it is extremely important to accurately detect fructo-oligosaccharides in infant dairy products. Nowadays, the main methods for detection of fructo-oligosaccharides at home and abroad include high performance liquid chromatography, ion exchange chromatography, mass spectrometry, etc. In this article, a method for the determination of fructo-oligosaccharides in modified milk and infant formula milk powder by high performance liquid chromatography (HPLC) was developed. Fructo-oligosaccharides was extracted by water. Protein was precipitated by acetonitrile. The method was performed on NH2 column, the detector was refractive index detector (RID) and the mobile phase was 70% acetonitrile. Fructo-oligosaccharides content of modified milk was in the range of 20-500mg/100g, the recovery was 80.2%-107.1%. The content of Fructo-oligosaccharides in infant formula milk powder was in the range of 100-500mg/100g, the recovery was 71.9%-95.3%. The quantitative detection limit was 20mg/100g. This method was easily to be operated with a high accuracy and the results indicated that it was suitable for the analysis of fructo-oligosaccharides in modified milk and infant formula milk powder.
Keywords
HPLC, Fructo-Oligosaccharides, Modified Milk, Infant Formula Milk Powder
To cite this article
Zhen Zhao, Guoyan Wen, Cuizhi Li, Peng Wan, Zhiyong Lv, HPLC Determination of Fructo-Oligosaccharides in Dairy Products, International Journal of Food Engineering and Technology. Vol. 2, No. 2, 2018, pp. 36-40. doi: 10.11648/j.ijfet.20180202.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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