<i>In-vitro</i> Antimicrobial, Anti-oxidant Activities and Cytotoxicty of <i>Carum carvi</i> L

Mohamed N. Abdalaziz; Mahmoud Mohamed Ali; Mohamed I. Garbi; Mohammed Abdalbagi Dafalla; Ahmed S. Kabbashi

doi:doi:10.11648/j.ajhc.20170303.11

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In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L

Mohamed N. Abdalaziz, Mahmoud Mohamed Ali, Mohamed I. Garbi, Mohammed Abdalbagi Dafalla, Ahmed S. Kabbashi

Published in American Journal of Heterocyclic Chemistry (Volume 3, Issue 3)

Received: 28 February 2017 Accepted: 19 April 2017 Published: 25 July 2017

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Abstract

Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC₅₀ less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.

Published in	American Journal of Heterocyclic Chemistry (Volume 3, Issue 3)
DOI	10.11648/j.ajhc.20170303.11
Page(s)	23-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Carum carvi L., Antimicrobial Activity, Anti-Oxidant Activity, Cytoxicity (Brine Shrimp)

References

[1]	Srivastava J, Lambert J and Vietmeyer N. Medicinal plants:An expanding role in development. World Bank Technical, 1996, 320.
[2]	Parekh J and Chanda S. In vitro antimicrobial activity of TrapanatansL. fruit rind extracted in different solvents. African Journal of Biotechnology, 6 (6), 2007, 760-770.
[3]	Rinoldi MG. Problems in the diagnosis of invasive fungaldiseases. Review of infectious diseases, 13, 1991, 493-495.
[4]	Meng JH, Zhao SH, Doyle MP, Joseph SW. Antibiotic resistance of Escherichia coli O157: H7 and O157: NM isolated from animals, food and humans. Journal of Food Protection, 61, 1998, 1511-1514.
[5]	Perreten V, Giampa N, Schuler-Schmid U, Teuber M. Antibiotic resistance genes in coagulase-negative staphylococci isolated from food. Systematic and Applied Microbiology, 21, 1998, 113-120.
[6]	Gruenwald J, Brendler T, Jaenicke C.2004. PDR for Herbal Medicines, 3rd Edition. Medical Economics Company 245-246.
[7]	Grieve M. 1984. A Modern Herbal. Penguin Books, Harmondsworth, London.
[8]	Bown D. 1995. Encyclopaedia of Herbs and Their Uses. DorlingKindersley, London.
[9]	Chevallier A. 1996. The Encyclopedia of Medicinal Plants. Dorling Kindersley. London.
[10]	Foster S. & Duke JA. 1990. A Field Guide to Medicinal Plants: Easternand Central North America. Houghton Mifflin Co, Boston.
[11]	Coffey T. 1993. The History and Folklore of North American WildFlowers. Facts on File, New York.
[12]	Tsarong TJ. 1994. Tibetan Medicinal Plants, 1st edn. Tibetan Medical Publications, Darjeeling.
[13]	Vasil IT.1970. Cruciferae. In Flora of the USSR (Komarov VL ed), p 240. Israel Program for Scientific Translations, Jerusalem.
[14]	Laribi, B., I. Bettaieb, K. Kouki, A. Sahli, A. Mougou and B. Marzouk, 2009. Water deficit effects on caraway (Carum carvi L.) growth, essential oil and fatty acid composition. Ind. Crop Prod., 30: 372-379.
[15]	National Committee for Clinical Laboratory Stan-dards (NCCLS) (1999). Performance standards for antimicrobial susceptibility testing; ninth infor-mational supplement. Wayne, Pensilvaniadocu-ment M100-S9, Vol. 19. No. 1.
[16]	Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992; 40:945–8.
[17]	Mayer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. and McLaughlin, J. L. (1982). Brine shrimp: a convenient bioassay for active plant constituents. PlantaMedica., 45: 31-34.
[18]	Kupahan, S. M., P. S. Steyn, M. D. Grove, S. M. Horsefield and S. W. Meitner, (1969). Tumor inhibitors, xxxv Myrsinesaponin. The active principle of Myrsineafricana. J. Med. Chem., 12: 167-169.
[19]	Ma, W. W., J. E. Anderson, C. J. Chang, D. L. Smith and J. L. McLaughlin, 1989. Majorenolide and majorynolide. A new pair of cytotoxic and pesticidal alkene-alkyne δ-lactones from Persia major. J. Nat. Pdts, 52: 1265-1266.
[20]	Oladimeji, H. O, Nia, R and Essien, E. E. (2006). In-vitro Anti-Microbial and Brine-Shrimp Lethality Potential of the Leaves and Stem of Calotropisprocera (Ait). African Journal of Biomedical Research, 9: 205 – 211.
[21]	Seidler-Lozykowska K, Kedzia B, Karpinska E and Bocianowski J. Microbiological activity of caraway (Carumcarvi L.) essential oil obtained from different origin. ActaScientiarum Maringa, 2013; 35 (4): 495-500.
[22]	Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26spice extracts and characterization of their phenolic constituents. Journal of Agricultural and Food Chemistry. 53, 2005, 7749-7759.

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APA Style

Mohamed N. Abdalaziz, Mahmoud Mohamed Ali, Mohamed I. Garbi, Mohammed Abdalbagi Dafalla, Ahmed S. Kabbashi. (2017). In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. American Journal of Heterocyclic Chemistry, 3(3), 23-27. https://doi.org/10.11648/j.ajhc.20170303.11

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ACS Style

Mohamed N. Abdalaziz; Mahmoud Mohamed Ali; Mohamed I. Garbi; Mohammed Abdalbagi Dafalla; Ahmed S. Kabbashi. In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. Am. J. Heterocycl. Chem. 2017, 3(3), 23-27. doi: 10.11648/j.ajhc.20170303.11

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AMA Style

Mohamed N. Abdalaziz, Mahmoud Mohamed Ali, Mohamed I. Garbi, Mohammed Abdalbagi Dafalla, Ahmed S. Kabbashi. In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. Am J Heterocycl Chem. 2017;3(3):23-27. doi: 10.11648/j.ajhc.20170303.11

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@article{10.11648/j.ajhc.20170303.11,
  author = {Mohamed N. Abdalaziz and Mahmoud Mohamed Ali and Mohamed I. Garbi and Mohammed Abdalbagi Dafalla and Ahmed S. Kabbashi},
  title = {In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L},
  journal = {American Journal of Heterocyclic Chemistry},
  volume = {3},
  number = {3},
  pages = {23-27},
  doi = {10.11648/j.ajhc.20170303.11},
  url = {https://doi.org/10.11648/j.ajhc.20170303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhc.20170303.11},
  abstract = {Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.},
 year = {2017}
}

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TY  - JOUR
T1  - In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L
AU  - Mohamed N. Abdalaziz
AU  - Mahmoud Mohamed Ali
AU  - Mohamed I. Garbi
AU  - Mohammed Abdalbagi Dafalla
AU  - Ahmed S. Kabbashi
Y1  - 2017/07/25
PY  - 2017
N1  - https://doi.org/10.11648/j.ajhc.20170303.11
DO  - 10.11648/j.ajhc.20170303.11
T2  - American Journal of Heterocyclic Chemistry
JF  - American Journal of Heterocyclic Chemistry
JO  - American Journal of Heterocyclic Chemistry
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2575-5722
UR  - https://doi.org/10.11648/j.ajhc.20170303.11
AB  - Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.
VL  - 3
IS  - 3
ER  -

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Author Information

Mohamed N. Abdalaziz

Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa, Khartoum, Sudan; Medicinal and Aromatic Plants and Traditional Medicine Research Institute (MAPTMRI), National Center for Research, Khartoum, Sudan
Mahmoud Mohamed Ali

Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa, Khartoum, Sudan
Mohamed I. Garbi

Department of Microbiology, Faculty of Medical Laboratory Sciences, International University of Africa, Khartoum, Sudan
Mohammed Abdalbagi Dafalla

Medicinal and Aromatic Plants and Traditional Medicine Research Institute (MAPTMRI), National Center for Research, Khartoum, Sudan
Ahmed S. Kabbashi

Department of Microbiology, Faculty of Medical Laboratory Sciences, International University of Africa, Khartoum, Sudan; Medicinal and Aromatic Plants and Traditional Medicine Research Institute (MAPTMRI), National Center for Research, Khartoum, Sudan

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APA Style

Mohamed N. Abdalaziz, Mahmoud Mohamed Ali, Mohamed I. Garbi, Mohammed Abdalbagi Dafalla, Ahmed S. Kabbashi. (2017). In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. American Journal of Heterocyclic Chemistry, 3(3), 23-27. https://doi.org/10.11648/j.ajhc.20170303.11

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ACS Style

Mohamed N. Abdalaziz; Mahmoud Mohamed Ali; Mohamed I. Garbi; Mohammed Abdalbagi Dafalla; Ahmed S. Kabbashi. In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. Am. J. Heterocycl. Chem. 2017, 3(3), 23-27. doi: 10.11648/j.ajhc.20170303.11

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AMA Style

Mohamed N. Abdalaziz, Mahmoud Mohamed Ali, Mohamed I. Garbi, Mohammed Abdalbagi Dafalla, Ahmed S. Kabbashi. In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L. Am J Heterocycl Chem. 2017;3(3):23-27. doi: 10.11648/j.ajhc.20170303.11

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@article{10.11648/j.ajhc.20170303.11,
  author = {Mohamed N. Abdalaziz and Mahmoud Mohamed Ali and Mohamed I. Garbi and Mohammed Abdalbagi Dafalla and Ahmed S. Kabbashi},
  title = {In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L},
  journal = {American Journal of Heterocyclic Chemistry},
  volume = {3},
  number = {3},
  pages = {23-27},
  doi = {10.11648/j.ajhc.20170303.11},
  url = {https://doi.org/10.11648/j.ajhc.20170303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhc.20170303.11},
  abstract = {Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.},
 year = {2017}
}

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TY  - JOUR
T1  - In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L
AU  - Mohamed N. Abdalaziz
AU  - Mahmoud Mohamed Ali
AU  - Mohamed I. Garbi
AU  - Mohammed Abdalbagi Dafalla
AU  - Ahmed S. Kabbashi
Y1  - 2017/07/25
PY  - 2017
N1  - https://doi.org/10.11648/j.ajhc.20170303.11
DO  - 10.11648/j.ajhc.20170303.11
T2  - American Journal of Heterocyclic Chemistry
JF  - American Journal of Heterocyclic Chemistry
JO  - American Journal of Heterocyclic Chemistry
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2575-5722
UR  - https://doi.org/10.11648/j.ajhc.20170303.11
AB  - Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.
VL  - 3
IS  - 3
ER  -

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