International Journal of Applied Agricultural Sciences
Volume 2, Issue 1, January 2016, Pages: 12-16
Received: Dec. 29, 2015;
Accepted: Jan. 4, 2016;
Published: Jan. 23, 2016
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Ibrahim Ismail Al-Mashhadani, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Duha Mysire Majeed, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Eman Noaman Ismail, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Maysaa Sameer Kadhim, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.
Ibrahim Ismail Al-Mashhadani,
Duha Mysire Majeed,
Eman Noaman Ismail,
Maysaa Sameer Kadhim,
Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions, International Journal of Applied Agricultural Sciences.
Vol. 2, No. 1,
2016, pp. 12-16.
Sairam, R. K. and Tyagi, A. (2004). Physiological and molecular biology of salinity stress tolerance in plants. Curr. Sci. 86(3): 407–421.
Al-Mishhadani, I. I. H. (2012). Breeding and selection of some Lines of Bread Wheat for salt tolerance. Journal of Agricultural Science and Techbology B. 2(8): 934-939.
Al-Mishhadani, I. I. H.; Abdula, K. H.; Isamil, E. N.; Thahre, Y. D. and Weab, I. A. (2014). Estimation of new wheat genotypes for salt tolerance which induced through plant breeding programs. Journal of Agricultural Science and Techbology B. 4 (2): 150-156.
Tester, K. M. R. and Roy, S. J. (2009). Quantifying the three main components of salinity tolerance in cereals. Plant, Cell and Environment. 32, 237-249.
Wang, W.; Vinocur, B. and Altman, A. (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta; 218: 1-14.
Chen, G. P.; Ma, W. S.; Huang, Z. J.; Xu, T.; Xue, Y. B. and Shen, Y. Z. (2003). Isolation and characterization of TaGSK1 involved in wheat salt tolerance. Plant Sci. 165, 1369–1375.
Ge, R-C, Chen, G-P, Zhao, B-C, Shen, Y-Z and Huang, Z-J (2012). Cloning and functional characterization of a wheat serine/threonine kinase gene ( TaSTK) related to salt-resistance. Plant Science 173, 55-60.
Ismail, E. N. (2013). Determination of gene expression of salt tolerant gene TaGSK1 in wheat cultivars Triticum aestivum L. Baghdad University.
Majeed, D. M.; Shwkat, M. S. and Sabbah, M. A. (2014). Determination of TaSC salt tolerance gene expression in selected wheat under different salt stresses. Egypt. Acad. J. Biolog. Sci. 5(1): 105-113.
Al-Mishhadani, I. I. H.; Zakariya, B. F.; Ismail, E. N. and Wisam, M. D. (2015). Detection for salt tolerance character in two selected genotypes of wheat. International Journal of Biology. 7(1): 54-60.
Gao, Z.; He, X.; Zhao, B. (2010) Overexpressing a putative aquaporin gene from wheat, TaNIP, enhances salt tolerance in transgenic Arabidopsis. Plant Cell Physiol.; 51(5): 767–775.
Guang Y, Yu F, and Liang D, (2011). Molecular cloning of a novel GSK3/shaggy-like gene from Triticum monococcum L. and its expression in response to salt, drought and other abiotic stresses. Afr. J. Biotechnol. 10 (20): 4065-4071.
Livak K, and Schmittgen T, (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 25: 402-408.
Barkla, B. J. and Pantoja, O. (1996) physiology of ion transport across the tonoplast of higher plants. Annu. Rev. Plant physiol. 47: 159-184.
Munns M, (2005). Genes and salt tolerance: bringing them together. New Phytologist; 167: 645-663.
Cuin, T. A.; Betts, S. A.; Chalmandrier, R. and Shabala, S (2008) A root’s ability to retain K+ correlates with salt tolerance in wheat. J. Exp. Bot.; 59: 2697–2706.
Cabanero, F. J., Martinez-Bellest, M. C. and Teruel, J. A. (2005) new evidence about the relationship between water channel activity and calcium in salinity – stressed pepper plants. Plant physiol. 13: 745-752.
Sheen, j. (1996) Ca+2 dependent protein kinases and stress signal transduction in plants. Science 274: 1900-1902.
Al-Mishhadani, I. I. H. (2015) Estimation of salt tolerance degree in some selected wheat genotypes by using detection of salt tolerant gene (TaSTK) and its expression under salinity conditions. Int. of Appl. Agric. Sci. 1 (2): 31-35.
Al-Mishhadani, I. I. H; ismail, E. N.; Jaddoa, K. A.; Majeed, D. M. and Mohammed, O. A (2015) Estimation of the interaction effect between salinity and growth regulators on salt tolerance of two bread wheat cultivar. Int. of Appl. Agric. Sci. 1 (4): 95-101.