Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum
International Journal of Applied Agricultural Sciences
Volume 2, Issue 6, November 2016, Pages: 79-82
Received: Jul. 25, 2016; Accepted: Sep. 14, 2016; Published: Oct. 14, 2016
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Endang Nurcahyani, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Rochmah Agustrina, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Erdi Suroso, Dept. of Agricultural Technology, Faculty of Agriculture, University of Lampung, Bandar Lampung, Indonesia
Gardis Andari, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
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Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.
Spathoglottis plicata, Induced Resistance, Fusarium oxysporum, Peroxidase Activity, Total Phenol
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Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari, Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum, International Journal of Applied Agricultural Sciences. Vol. 2, No. 6, 2016, pp. 79-82. doi: 10.11648/j.ijaas.20160206.11
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