Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum
International Journal of Applied Agricultural Sciences
Volume 2, Issue 6, November 2016, Pages: 79-82
Received: Jul. 25, 2016; Accepted: Sep. 14, 2016; Published: Oct. 14, 2016
Views 3653      Downloads 129
Authors
Endang Nurcahyani, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Rochmah Agustrina, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Erdi Suroso, Dept. of Agricultural Technology, Faculty of Agriculture, University of Lampung, Bandar Lampung, Indonesia
Gardis Andari, Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Article Tools
Follow on us
Abstract
Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.
Keywords
Spathoglottis plicata, Induced Resistance, Fusarium oxysporum, Peroxidase Activity, Total Phenol
To cite this article
Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari, Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum, International Journal of Applied Agricultural Sciences. Vol. 2, No. 6, 2016, pp. 79-82. doi: 10.11648/j.ijaas.20160206.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Aberouman A. A and Deokule S. S. 2008. Comparison of Phenolic Compounds of Some Edible Plants of Iran and India. Pakistan Journal of Nutrition 7(4): 582-585.
[2]
Arai M and Takeuchi M. 1993. Influence of Fusarium Wilt toxin (s) on Carnation cell. Plant Cells, Tissue and Organ Culture (34): 287–293.
[3]
Bacon CW, Porter JK, Norred WP dan Leslie JF. 1996. Production of Fusaric Acid by Fusarium sp. Applied and Enviromental Microbiology. 62 (11): 4039-4043.
[4]
Bouizgarne B, Bouteau HEM, Frankart C, Reboutier D, Madiona K, Pennarun AM, Monestiez M, Trouverie J, Amiar Z, Briand J, Brault M, Rona JP, Ouhdouch Y, & Hadramu EI. 2006. Early Physiological Responses of Arabidopsis thaliana Cells to Fusaric Acid: Toxic and Signallling Effects. New Phytologist 169: 209–218.
[5]
Goodman RN, Zoltan K, and Milton Z, 1986. The Biochemistry and Physiology of Plant Disease. D. Van Nostrand Company, Inc. New Jersey, Toronto, London, Melbourne.
[6]
Hadisutrisno, B. 1995. Taktik dan Strategi Perlindungan Tanaman Menghadapi Gangguan Penyakit Layu Fusarium. Makalah Simposium Nasional I di Purwokerto, 2-3 Maret.
[7]
Khan I. A, Alam S. S, Haq A, & Jabbar A. 2005. Biochemistry of Resistance in Chicpea Against Wilt Disease Caused by Fusarium oxysporum f. sp. ciceris. Pak. J. Bot. 37: 97-104.
[8]
Landa BB, Cachinero-Diaz JM, Lemanceu P, Jimenez-Diaz RM & Alabouvette C. 2002. Effect of fusaric acid and phytoanticipans on growth of rhizobacteria and Fusarium oxysporum. Canadian Journal of Microbiology 48: 971-985.
[9]
Nurcahyani E, Sumardi I, Hadisutrisno B, & Suharyanto E. 2012. Supression of Development of Vanilla Foot Rot Disease (Fusarium oxysporum f. sp. vanillae) Throught In Vitro Fusaric Acid Selection. Journal of Tropical Plant Pests and Diseases. Acreditation of DIKTI No.110/DIKTI/Kep/2009. ISSN: 1411-7525. Vol. 12 /No. 1: 12-22.
[10]
Palmer GD. 2011. The control of orchids. http://www.ebow.com/info_8525784_control_fusarium-wilt-orchids html. Accesed on January 20, 2016.
[11]
Remotti PC, Lofler HJM & Lotten-Doting LV. 1997. Selection of cell lines and regeneration of plants resistance to fusaric acid from Gladiolus x gradiflorus c.v ‘Peteer Pear’. Euphytica 96: 237-245.
[12]
Ruzin, SE. 1999. Plant Microtechnique and Microscopy. Oxford University Press, New York.
[13]
Saravanan T, Bhaskaran R, and Muthusamy M. 2004. Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (cv. Rasthali) against Fusarium Wilt Disease. Plant Pathology Journal. 3: 72-80.
[14]
Sticher L, Mauch-Mani B and Metraux JP. 1997. Systemic acquired resistance. Annual Review Phytopathology 35: 235-270.
[15]
Toyoda H, Hasyashi H and Yamamoto K. 1984. Selection of resistant tomato calli to fusaric acid. Ann. Phytopathol. Soc. Japan 50: 538–540.
[16]
Umamaheswari R and Mohanan K. V. 2009. A Study of the Asociation of Agronomic Characters in Vanilla planifolia Andrews. International Journal of Plant Breeding and Genetics 5 (1): 53-58.
[17]
Vidhyasekaran P. 1997. Fungal Pathogenesis in Plants and Crops, Molecular Biology and Host Defense mechanism. Marcel Dekker. New York. 553 p.
[18]
Windels CE. 1993. Fusarium. pp. 115-128 In: Methods for Research on Soilborne Phytopathogenic fungi. Singleton LL, Mihail JD & Rush CM. eds. APS Press. St. Paul, Minnesota.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186