Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)
Journal of Health and Environmental Research
Volume 4, Issue 4, December 2018, Pages: 153-160
Received: Nov. 10, 2018;
Accepted: Dec. 9, 2018;
Published: Jan. 22, 2019
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Timothy Omara, Department of Health Sciences, Unicaf University, Lusaka, Zambia; Department of Quality Control, Quality Assurance and Product Development, AgroWays Uganda Limited, Jinja, Uganda; Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda; Department of Quality Control and Quality Assurance, Leading Distillers Uganda Limited, Kampala, Uganda
Fredrick Kizza Kateeba, Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda; Department of Quality Control and Quality Assurance, Leading Distillers Uganda Limited, Kampala, Uganda
Bashir Musau, Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda; Department of Quality Control and Quality Assurance, Leading Distillers Uganda Limited, Kampala, Uganda
Erisa Kigenyi, Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda; Department of Quality Control and Quality Assurance, Leading Distillers Uganda Limited, Kampala, Uganda
Eddie Adupa, Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda; Department of Quality Control and Quality Assurance, Abacus Parenteral Drugs Limited, Mukono, Uganda
Sarah Kagoya, Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda
Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.
Fredrick Kizza Kateeba,
Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), Journal of Health and Environmental Research.
Vol. 4, No. 4,
2018, pp. 153-160.
B. Speiser. ''Molluscides''. In: Encyclopedia of Pest management, vol. 219, pp. 506-08, 2002.
J. Kulkarni, N. Kapse and D. K. Kulkarni. ''Plant-based pesticides for control of Helicoverpa amigera on Cucumis''. Asian Agricultural History, vol. 13, no. 4, 327-332, 2009
The Market Linkages Initiative (MLI). ''Crop Conditioning Handbook''. pp. 1-31, May 2010.
M. D. Moreira, M. C. Picanço, L. C. A. Barbosa, R. N. C. Guedes, E. M. Silva. ''Toxicity of leaf extracts of Ageratum conyzoides to lepidoptera pests of horticultural crops''. Biol. Agric. Hortic., vol. 22, pp. 251–60, 2004.
M. D. Moreira, M. C. Picanço, L. C. A. Barbosa, R. N. C. Guedes, M. R. Campos. ''Compounds from Ageratum conyzoides: Isolation, structural elucidation and insecticide activity''. Pest Management Science, vol. 63, pp. 615–21.
M. D. Moreira, M. C. Picanço, L. C. A. Barbosa, R. N. C. Guedes, M. R. Campos, G. A. Silva, J. C. Martins.'' Plant Compounds insecticide activity against Coleoptera pests of stored products''. Pesq. Agropec. Bras., vol. 42, pp. 909–15, 2007
N. Aarthi, K. Murugan. ''Larvicidal and repellent activity of Vetiveria zizanioides L, Ocimum basilicum L and the microbial pesticide spinosad against malarial vector, Anopheles stephensi Liston (Insecta: Diptera: Culicidae)''. J Biopest, vol. 3, pp. 199–204, 2010.
M. Govindarajan. ''Larvicidal and repellent properties of some Essential Oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae)''. Asian Pacific Journal of Tropical Medicine, vol. 4, pp. 106–11, 2011.
M. Govindarajan. ''Chemical composition and larvicidal activity of leaf essential oil from Clausena anisate (Willd.) Hook. f. ex Benth (Rutaceae) against three mosquito species''. Asian Pacific Journal of Tropical Diseases, vol. 3, pp. 874–77, 2010.
M. Govindarajan, R. Sivakumar. Repellent properties of Cardiospermum halicacabum Linn. (Family: Sapindaceae) plant leaf extracts against three important vector mosquitoes''. Asian Pacific Journal of Tropical Diseases, vol. 8, pp. 602–07, 2012.
K. Krishnappa, K. Elumalai. ''Toxicity of Aristolochia bracteata methanol leaf extract against selected medically important vector mosquitoes (Diptera: Culicidae)''. Asian Pacific Journal of Tropical Diseases, vol. 2, S553–S557, 2002.
S. Phasomkusolsil, M. Soonwera, M. ''Efficacy of herbal Essential oils as insecticide against Aedes aegypti (Linn.), Culex quinquefasciatus (Say.) and Anopheles dirus (Peyton and Harrison)''. Southeast Asian Journal of Tropical Medicine and Public Health, vol. 42, pp. 1083–92, 2011.
W. Choochote, U. Chaithong, K. Kamsuk, A. Jitpakdi, P. Tippawangkosol, B. Tueton, D. Champakaew, B. Pitasawat. ''Repellent activity of selected Essential oils against Aedes aegypti''. Fitoterapia, vol. 78, pp. 359-64, 2007.
K. Murugan, P. Murugan, A. Noortheen. ''Larvicidal and repellent potential of Albizzia amara Boivin and Ocimum basilicum Linn against dengue vector, Aedes aegypti (Insecta: Diptera: Culicidae)''. Bioresource Technolology, vol. 98, pp. 198–201, 2007.
J. Gokulakrishnan, E. Kuppusamy, D. Shanmugam, A. Appavu, K. Kaliyamoorthi. ''Pupicidal and repellent activities of Pogostemon cablin essential oil chemical compounds against medically important human vector mosquitoes''. Asian Pacific Journal of Tropical Diseases, vol. 3, pp. 26–33, 2013.
S. Tennyson, J. Ravindran, A. Eapen, J. Willian. ''Repellent activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)''. Asian Pacific Journal of Tropical Diseases, vol. 6, pp. 478–80, 2002.
S. Mandal. ''Repellent activity of Eucalyptus and Azadirachta indica seed oil against the filarial mosquito Culex quinquefasciatus Say. (Diptera: Culicidae) in India''. Asian Pacific Journal of Biomedicine, vol. 1, S109–S112, 2011.
S. Tjahjani. ''Efficacy of several Essential oils against Culex and Aedes repellents''. Proceedings of ASEAN Congress Tropical Medical Parasitology, vol. 3, 33–37, 2008.
N. Sophia and R. S. Pandian. ''Screening of the efficacy of phytochemical repellents against the filarial vector mosquito, Culex quinquefasciatus Say''. Current Biotechnology, vol. 3, pp. 14–31, 2009.
A. Ali, C. C. Murphy, B. Demirci, D. W. Wedge, B. J. Sampson, I. A. Khan, K. H. C. Baser, N. Tabanca. ''Insecticidal and biting deterrent activity of rose-scented geranium (Pelargonium spp.) Essential oils and individual compounds against Stephanitis pyrioides and Aedes aegypti''. Pest Management Science, vol. 69, pp. 1385–92, 2013.
N. Tabanca, U. R. Bernier, A. Ali, M. Wang, B. Demirci, E. K. Blythe, S. I. Khan, K. H. C. Baser, I. A. Khan, I. ''Bioassay-guided investigation of two Monarda Essential oils as repellents of yellow fever mosquito, Aedes aegypti''. J Agric Food Chem, vol. 61, pp. 8573–80, 2013.
D. E. Wedge, J. A. Klun, N. Tabanca, B. Demirci, T. Ozek, K. H. C. Baser, Z. Liu, S. Zhang, C. L. Cantrell, L. Zhan. ''Bioactivity-guided fractionation and GC/MS fingerprinting of Angelica sinensis and Angelica archangelica root components for antifungal and mosquito deterrent activity''. J Agric Food Chem, vol. 57, pp. 464–70, 2009.
UNEP. ‘‘Ridding the World of POPs’’. In: A guide to the Stockholm Convention on Persistent Organic Pollutants, 2005.
E. Y. A. Abdel Daffie, E. A. Elhaj, N. H. H. Bashir. ‘‘Resistance in the cotton whitefly, Bemisiatabaci (Genn.) to insecticides recently introduced into Sudan Gezira’’. Tropical Pest Management, vol. 33, no. 4, 283-286, 1987.
R. Pavela. ''Larvicidal effects of some Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae).'' Parasitol Res, vol. 105, pp. 887–92, 2009.
Trees portal. ''Eucalytpus saligna Smith''. Available at www.treesportal.com. Retrieved 21st January 2017.
L. G. Batista-Pereira, J. B. Fernandes, A. G. Correa, M. F. G. F. da Silva, P. C. Vieira. '' Electrophysiological responses of Atta sexdens rubropilosa workers to Essential oils of Eucalyptus and its chemical composition. Z. Naturforsch, vol. 61, pp. 749–55, 2006.
L. G. Batista-Pereira, J. B. Fernandes, A. G. Correa, M. F. G. F. da Silva, P. C. Vieira. ''Electrophysiological responses of Eucalyptus brown looper Thyrinteina arnobia to Essential Oils of seven Eucalyptus species''. Journal of Brazilian Chemists Society, vol. 17, pp. 555–61, 2006.
P. Bett, A. L. Deng, J. O. Ogendob, S. T. Kariuki, M. Kamatenesi-Mugisha, J. M. Mihale, B. Torto. ''Chemical composition of Cupressus lusitanica and Eucalyptus saligna leaf essential oils and bioactivity against major insect pests of stored food grains''. Indust Crop Prod, vol. 82, pp. 51–62, 2016.
A. A. Estanislau, F. A. S. Barros, A. P. Peña, S. C. Santos, P. H. Ferri, J. R. Paula. ‘‘Chemical composition and antibacterial activity of the essential oils of five species of Eucalyptus grown in Goiás’’. Revista Brasileira de Farmacognosia, vol. 11, pp. 95–100, 2011.
A. O. Oyedeji, O. Ekundayo, O. N. Olawore, B. A. Adeniyi, W. A. Koenig. ''Antimicrobial activity of the Essential oils of five Eucalyptus species growing in Nigeria''. Fitoterapia, vol. 70, pp. 526–28, 1999.
P. Sartorelli, A. D. Marquioreto, A. Amaral-Baroli, M. Enoque, L. Lima, P. Roberto, H. Moreno. ''Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus''. Phytother Res, vol. 21, no. 23, pp. 231-33, 2007.
K. Cimanga, K. Kambu, L. Tona, S. Apers, T. de Bruyne, N. Hermans, J. Totté, L. Pieters, A. J. Vlietinck. ''Correlation between chemical composition and antibacterial activity of Essential Oils of some aromatic medicinal plants growing in the Democratic Republic of Congo''. J Ethnopharmacol, vol. 79, pp. 213–220, 2002.
C. A. Filomeno, L. C. A. Barbosa, J. L. Pereira, A. L. Pinheiro, P. H. Fidencio, R. M. Montanari. ''The chemical diversity of Eucalyptus spp. essential oils from plants grown in Brazil'. Chemical Biodiversity, 2008.
Y. G. Gillij, R. M. Gleiser, J. A. Zygadlo. ''Mosquito repellent activity of Essential oils of aromatic plants growing in Argentina''. Bioresource Technology, vol. 99, pp. 2507–15, 2008.
A. J. Mossi, V. Astolfi, G. Kubiak, V. G. Lerin, C. Zanella, G. Toniazzo, D. Oliveira, H. Treichel, I. A Devilla, R. Cansiana, R. Restello. ''Insecticidal and repellency activity of essential oil of Eucalyptus sp. Against Sitophilus zeamais Motschulsky (Coleoptera, Curculionidae)''. J Food Sci Agric, vol. 91, pp. 273–77, 2011.
A. C. Toloza, J. Zygadlo, C. G. Mougabure, F. Biurrun, E. Zerba, M. I. Picollo. ''Fumigant and repellent properties of Essential Oils and component compounds against permethrin-resistant Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina''. J Med Entomol, vol. 43, pp. 889–95, 2006.
A. Lucia, S. Licastro, E. Zerba, H. Masuh, H. Yield. ''Chemical composition, and bioactivity of Essential oils from 12 species of Eucalyptus on Aedes aegypti larvae''. Entomologia Experimentalis et Applicata, vol. 129, pp. 107–14, 2008.
A. Lucia, S. Licastro, E. Zerba, A. P. Gonzalez, H. Masuh. ''Sensitivity of Aedes aegypti adults (Diptera: Culicidae) to the vapors of Eucalyptus Essential oils''. Biores Tech, vol. 100, pp. 6083–87, 2009.
S. Lee, C. J. Peterson, J. R. Coats. Fumigation Toxicity of Monoterpenoids to several stored product pests. J Stored Prod Res, vol. 39, no. 1,pp. 77-85, 2002.
S. Rajendran and V. Sriranjini. ''Plant products as fumigants for stored-product insect control''. Journal of Stored Products and Resources, vol. 44, pp. 126–35, 2008.
P. J. Houghton, Y. Ren, M. J. Howes. ''Acetylcholinesterase inhibitors from plants and fungi''. Natural Products Rep., vol. 23, pp. 181–99, 2006.
J. S. Zhang, N. N. Zhao, Q. Z. Liu, et al. “Repellent constituents of essential oil of Cymbopogon distans aerial parts against two stored-product insects”. Journal of Agricultural Food Chemistry, vol. 59, no. 18, pp. 9910–9915, 2011.
A. Y. Abdelghany, S. S. Awadalla, N. F. Abdel-Baky, H. A. El-Syrafi and P. G. Fields. “Stored-product insects in botanical warehouses”. Journal of Stored Products Research, vol. 46, no. 2, pp. 93–97, 2010.
Z. L. Liu, S. H. Ho. “Bioactivity of the essential oil extracted from Evodia rutaecarpa Hook f. et Thomas against the grain storage insects, S. zeamais Motsch. and Tribolium castaneum (Herbst)''. J Stored Prod Res, vol. 35, no. 4, pp. 317-28, 1999.
L. A. Gonçalves, L. C. A. Barbosa, A. A. Azevedo and V. W. D. Casali.''Production and composition of essential oil of alfavaquinha (Ocimum selloi Benth) in response to two levels of solar radiation. Brazilian J Med Plants, vol. 6, 8-14, 2003.
F. Bakkali, S. Averbeck, D. Averbeck, M. Idaomar. ''Biological Effects of Essential oils-A review''. Food Chemical Toxicology, vol. 46, pp. 446–75, 2008.