Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes
International Journal of Biomedical Engineering and Clinical Science
Volume 2, Issue 1, November 2016, Pages: 8-17
Received: Nov. 29, 2016; Accepted: Dec. 26, 2016; Published: Jan. 7, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
William Dean Plikerd, Trivedi Global, Inc., Henderson, Nevada, USA
Peter L. Surguy, Trivedi Global, Inc., Henderson, Nevada, USA
Robert John Kock, Trivedi Global, Inc., Henderson, Nevada, USA
Rolando Baptista Piedad, Trivedi Global, Inc., Henderson, Nevada, USA
Russell Phillip Callas, Trivedi Global, Inc., Henderson, Nevada, USA
Sakina A. Ansari, Trivedi Global, Inc., Henderson, Nevada, USA
Sandra Lee Barrett, Trivedi Global, Inc., Henderson, Nevada, USA
Sara Friedman, Trivedi Global, Inc., Henderson, Nevada, USA
Steven Lee Christie, Trivedi Global, Inc., Henderson, Nevada, USA
Su-Mei Chen Liu, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Elizabeth Starling, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Jones, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Mardis Allen, Trivedi Global, Inc., Henderson, Nevada, USA
Susanne Kathrin Wasmus, Trivedi Global, Inc., Henderson, Nevada, USA
Terry Ann Benczik, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Charles Slade, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Orban, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria L. Vannes, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria Margot Schlosser, Trivedi Global, Inc., Henderson, Nevada, USA
Yusif Sarkis Yamin Albino, Trivedi Global, Inc., Henderson, Nevada, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pr
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pr
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Abstract
With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Keywords
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Immune-Modulation, Pro-inflammatory Cytokines, Splenocytes
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Sambhu Charan Mondal, Snehasis Jana, Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes, International Journal of Biomedical Engineering and Clinical Science. Vol. 2, No. 1, 2016, pp. 8-17. doi: 10.11648/j.ijbecs.20160201.12
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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