International Journal of Biomedical Engineering and Clinical Science

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Effect of Co-Administration of Vitamin E Isoforms d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats

Received: 19 January 2017    Accepted: 15 February 2017    Published: 31 October 2017
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Abstract

Normal wound healing involves sequence of events which is believed to be altered in diabetes due to hyperglycemia, infection and oxidative stress. The latter may be reduced by antioxidants which neutralize the chain formation of free radicals. Vitamin E is a well-known antioxidant and has saturated tocopherols and unsaturated tocotrienols. The most active form being the α-tocopherol. The present study was designed to explore the combined effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on wound healing process in both healthy and alloxan-induced diabetic rats. Diabetes was induced with alloxan (100 mg/kg S. C). Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each orally and daily for 3 weeks. Under general anesthesia, full-thickness excisional skin wounds were created on the dorsal surface of thoracic region. Macroscopic and microscopic features of wound healing stages were recorded at weekly intervals and biochemical parameters were estimated at the end of 3 weeks. It was observed that as compared to control in the treated group there was early reappearance of epidermal and dermal components, reduced serum creatinine level, increased serum antioxidant status and total protein content and controlled glycemic status. It is concluded that oral co-administration of d-α-tocopherol and d-δ-TRF promotes skin wound healing in both healthy and diabetic rats through its antioxidant potency, therefore suggested that vitamin E isoforms hold promising future in the effective management of wounds in both otherwise healthy and diabetics.

DOI 10.11648/j.ijbecs.20170305.11
Published in International Journal of Biomedical Engineering and Clinical Science (Volume 3, Issue 5, September 2017)
Page(s) 52-62
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antioxidant, d-α-Tocopherol, d-δ-Tocotrienol Rich Fraction, Diabetes, Skin, Wounds

References
[1] Latiff AA, Lin TS, and Das S. Wound healing in diabetes mellitus: traditional treatment modalities. ClinicaTerapeutica, 161, 2010, 359-364.
[2] Karasu C, Ozansoy G, Bozkurt O, Erdo_and, Omerolus S. Antioxidant and Triglyceride-lowering effects of vitamin E associated with the prevention of abnormalities in the reactivity and morphology of aorta from streptozotocin-diabetic rats. Antioxidants in Diabetes-Induced Complications (ADIC) Study Group. Metabolism, 46, 1997, 46, 872-79.
[3] Peerapatdit T, Likidlilid A, Patchanans N, Somkasetrin A. Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus. J Med Assoc Thai, 89, 2006, S141-46.
[4] Yoshida Y, Niki E and Noguchi N. Comparative study on the action of tocopherols and tocotrienols as antioxidants: chemical and physical effects. Chem Phys Lipids, 123, 2003, 63-75.
[5] Thiele JJ, Hsieh SN and Ekanayake-Mudiyanselage S. Vitamin E: critical review of its current use in cosmetic and clinical dermatology. Dermatologic Surgery, 31, 2005, 31, 805-813.
[6] Serbinova EA, Kagan VE, Han D, Packer L. Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol. Free RadicBiol Med, 10, 1991, 263-75.
[7] Ahsan H, Ahad A, Iqba J and Siddiqui WA. Pharmacological potential of tocotrienols: a review. Nutrition & Metabolism, 52, 2014, 1-22.
[8] Packer L. Protective role of vitamin E in biological systems. Am J ClinNutr, 53, 1991, 1050S-1055S.
[9] Elson CE. Tropical oils: Nutritional and scientific issues. Crit Rev Food SciNutr, 31, 1992, 79-102.
[10] McIntyre BS, Briski KP, Tirmenstein MA, Fariss MW, Gapor A, Sylvester PW: Antiproliferative and apoptotic effects of tocopherols and tocotrienols on normal mouse mammary epithelial cells. Lipids, 35, 2000, 171-180.
[11] McIntyre BS, Briski KP, Gapor A, Sylvester PW: Antiproliferative and apoptotic effects of tocopherols and tocotrienols on preneoplastic and neoplastic mouse mammary epithelial cells. ProcSocExpBiol Med, 224, 2000, 292-301.
[12] Sylvester PW, McIntyre BS, Gapor A, Briski KP: Vitamin E inhibition of normal mammary epithelialcell growth is associated with a reduction in protein kinase C (alpha) activation. Cell Prolif, 34, 2001, 347- 357.
[13] Zaini AA, Khaza’ai H, Ali RM, Abdul Mutalib MS, Baharuddin AA. Topical Treatment of Tocotrienol-Rich Fraction (TRF) on Deep Partial-Thickness Burn Wounds in Rats. J DermatologClin Res, 4, 2016, 1063-70.
[14] Musalmah M, MuhdFairuz AH, GaporMTand Wan Ngah WZ. Effect of tocotrienol-rich fraction on wound healing in streptozotocin-induced diabetic rats. Malaysian J BiochemMolBiol, 6, 2001, 34-39.
[15] Fatmah A. Matough, Budin SB, Zariyantey AH, Mariati AR, Nasar Al-Wahaibi and Jamaludine M. Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats. Sultan Qaboos University Med J, 14, 2014, 95-103.
[16] Elsy B, Maheshwari V, Khan AA. Effects of d-α-Tocopherol on Progression of Reepithelialization, Matrix Remodeling and Appearance of Epidermal Append¬ages in Secondary Skin Wounds of Diabetic Rats. J DermatologClin Res, 4, 2016, 1081-1087.
[17] Lemo N, Marignac G, Reyes-Gomez E, Lilin T, Crosaz O, DohanEhreenfest M. Cutaneous reepithelialization and wound contraction after skin biopsies in rabbit: a mathematical model for healing and remodelling matrix. Vet. Arhiv, 80, 2010, 637-652.
[18] Sinha AK. Colorimetric assay of catalase. Anal Biochem, 47, 1972, 389-394.
[19] Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochem, 239, 1996, 70-76.
[20] Senel O, Cetinkale O, Ozbay G, Ahcioglu F, Bulan R. Oxygen free radicals impair wound healing in ischemic rat skin. Ann PlastSurg, 39, 1997, 517-523.
[21] Rosenbaum MA, Miyazaki K and Graham LM. Hypercholesterolemia and oxidative stress inhibit endothelial cell healing after arterial injury. J VascSurg, 55, 2012, 489-496.
[22] Lin TS, Latiff AA, Abd Hamid NA, Wan Ngah WZ btand Musalmah M. Evaluation of Topical Tocopherol Cream on Cutaneous Wound Healing in Streptozotocin-Induced Diabetic Rats. Evidence- Based Complementary and Alternative Medicine 2012; doi:10.1155/2012/491027. Hindawi Publishing Corporation: ID 491027.
[23] Musalmah M, Fairuz AH, Gapor MT and Ngah WZ. Effect of vitamin E onplasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats. Asia Pac J ClinNutr, 11, 2002, 448-451.
[24] Musalmah M, Nizrana MY, Fairuz AH, et al. Comparative effects of palm vitamin E and alpha-tocopherol on healing and wound tissue antioxidant enzyme levels in diabetic rats. I ipida, 40, 2005, 575-580.
[25] Azlina MF, Nafeeza MI and Khalid BA. A comparison between tocopherol and tocotrienol effects on gastric parameters in rats exposed to stress. Asia Pac. J. Clin. Nutr, 14, 2005, 358-365.
[26] Dauqan E, Sani HA, Abdullah A and Kasim ZM. Effect of different vegetable oils (red palm olein, palm olein, corn oil and coconut oil) on lipid profile in rat. Food NutrSci 2011; 2: 253-258. DOI:10.4236/fns.2011.24036.
[27] Sebastian S, Walter E M and Gunter PE. Tocotrienols: Constitutional Effects in Aging and Disease. Recent Advances in Nutritional Sciences. J. Nutr, 135, 2005, 151-154.
[28] Budin SB, Khairunnisa MY, MuhdHanis MI, Zariyantey AH, Jamaludin M. Tocotrienol-Rich Fraction of Palm Oil Reduced Pancreatic Damage and Oxidative Stress in Streptozotocin-Induced Diabetic Rats. Aust. J. basic appl. sci, 5, 2011, 2367-2374.
[29] Kuhad A, Bishnoi M, Tiwari V and Chopra K.2009. Suppression of NF-kappabeta signaling pathway by tocotrienol can prevent diabetes associated cognitive deficits. Pharmacol. Biochem. Behav, 92, 2009, 251-259.
[30] Escamez MJ, Garcia M, Larcher F, Meana A, Munoz E, Jorcano JL and Del RM. An in vivo model of wound healing in genetically modified skin-humanized mice. J Invest Dermatol, 123, 2004, 1182-91.
[31] Martin P. Wound healing–aiming for perfect skin regeneration. Science, 276, 1997, 75-81.
[32] Wysocki A B. Skin anatomy, physiology, and pathophysiology. NursClin North Am, 34, 1999, 777-97.
[33] Altavilla D, Saitta A, Cucinotta D, Galeano M, Deodato B, Colonna M, et al. Inhibition of Lipid Peroxidation Restores Impaired Vascular Endothelial Growth Factor Expression and Stimulates Wound Healing and Angiogenesis in the Genetically Diabetic Mouse. Diabetes, 50, 2001, 667-674.
[34] Peacock EE. Wound repair. In: Wound repair. Saunders, Philadelphia.1984, 38-55.
[35] Oikarinen A. Aging of the skin connective tissue: how to measure the biochemical and mechanical properties of aging dermis. PhotodermatolPhotoimmunolPhotomed, 10, 1994, 47-52.
[36] Sushma RK, Sreedhara K R Pai, Nayak JK, Hemalatha B, Keerthana P, Kumar MR Bhat. Biomechanical, biochemical and histological evidences for wound healing properties of indian traditional medicines. Int J Pharm PharmSci, 7, 2015, 163-171.
[37] Prost-Squarcioni C, Fraitag S, Heller M, Boehm N. Functional histology of dermis. Ann DermatolVenereol, 135, 2008, 1S5-20.
[38] Liora BW, Nessa S, Ram S and Tamar T. Novel Insights into Wound Healing Sequence of Events. Toxicologic Pathology, 35, 2007, 767-779.
[39] Chertow B, Edwards JC. Advances in Diabetes for the Milennium: Vitamins and Oxidant Stress in Diabetes and Its Complications. Medscape General Med, 6, 2004, 1-10.
[40] Cullen P, Eckardstein A, Souris S, Schulte H. Assmann G. Dyslipidaemia and cardiovascular risk in diabetes. Diabetes Obes. Metab, 1, 1999, 189-98.
[41] Solano MPMD, Goldberg RBMD. Management of dyslipidemia in diabetes. Cardiology in Review, 14, 2006, 125-35.
[42] Sout RW. Diabetes and athoresclerosis. Biomed. Pharmacother, 47, 2005, 1-2.
[43] Danielle AT de Almeida, Camila PB, Ethel LBNand Ana Angélica HF. Evaluation of Lipid Profile and Oxidative Stress in STZ Induced Rats Treated with Antioxidant Vitamin. Braz. Arch. BiolTechnol, 55, 2012, 527-536.
[44] Budin SB, Othman F, Louis SR, Bakar MA, Das S and Mohamed J. The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats. Clinics Sao Paulo, 64, 2009, 235-244.
[45] Ronco C, Grammaticopoulos S, Rosner M, Decal M, Soni, S, Lentini P, Oliguria. Creatinine and other biomarkers of acute kidney injury. Contributions Nephrol, 164, 2010, 118-27.
[46] Lin TS, Latiff AA and Das S. The effect of topical extract of Momordicacharantia(bitter gourd) on wound healing in nondiabetic rats and in rats with diabetes induced by streptozotocin. Clinical and Experimental Dermatology, 34, 2009, 815-822.
[47] Vasudevan DM and Sreekumari S. Textbook of Biochemistry (For Medical Students), 4th edition 2005, 340-341.
[48] Jeyashanthi N, Ashok V. Anti-Oxidative Effect of Cassia auriculata on Streptozotocin Induced Diabetic Rats. Ind J ClinBiochem, 25, 2010, 429-434.
[49] Shirpoor A, KhademAnsari MH, Salami S, GhaderiPakdel F, RasmiY. Effect of vitamin E on oxidative stress status in small intestine of diabetic rat. World J Gastroenterol, 13, 2007, 4340-4344.
[50] Brahm KT, Kanti BP, Abidi AB and Syed Ibrahim R. Markers of Oxidative Stress during Diabetes Mellitus. Journal of Biomarkers 2013, http://dx. doi. org/10.1155 /2013/378790; Hindawi Publishing Corporation: ID 378790.
[51] Cakatay U, Kayali R. The evaluation of altered redox status in plasma and mitochondria of acute and chronic diabetic rats. ClinBiochem, 39, 2006, 907-912.
[52] Ghada ZA, Soliman and Nehal MB. Effect of Vitamin C and/or Vitamin E on Oxidative Stress and Lipid Profile in Diabetic Rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3, 2012, 639-652.
[53] Alireza N, Bokaeian M, Mohsen S, Ali F and Azim A. Attenuation of oxidative stress in streptozotocin-induced diabetic rats by eucalyptus globulus. Indian Journal of Clinical Biochemistry, 24, 2009, 419-425.
Author Information
  • Department of Anatomy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India

  • Department of Anatomy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India

  • Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India

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    Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. (2017). Effect of Co-Administration of Vitamin E Isoforms d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats. International Journal of Biomedical Engineering and Clinical Science, 3(5), 52-62. https://doi.org/10.11648/j.ijbecs.20170305.11

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    Bijo Elsy; Aijaz Ahmed Khan; Veena Maheshwari. Effect of Co-Administration of Vitamin E Isoforms d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats. Int. J. Biomed. Eng. Clin. Sci. 2017, 3(5), 52-62. doi: 10.11648/j.ijbecs.20170305.11

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    AMA Style

    Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. Effect of Co-Administration of Vitamin E Isoforms d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats. Int J Biomed Eng Clin Sci. 2017;3(5):52-62. doi: 10.11648/j.ijbecs.20170305.11

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  • @article{10.11648/j.ijbecs.20170305.11,
      author = {Bijo Elsy and Aijaz Ahmed Khan and Veena Maheshwari},
      title = {Effect of Co-Administration of Vitamin E Isoforms  d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {3},
      number = {5},
      pages = {52-62},
      doi = {10.11648/j.ijbecs.20170305.11},
      url = {https://doi.org/10.11648/j.ijbecs.20170305.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijbecs.20170305.11},
      abstract = {Normal wound healing involves sequence of events which is believed to be altered in diabetes due to hyperglycemia, infection and oxidative stress. The latter may be reduced by antioxidants which neutralize the chain formation of free radicals. Vitamin E is a well-known antioxidant and has saturated tocopherols and unsaturated tocotrienols. The most active form being the α-tocopherol. The present study was designed to explore the combined effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on wound healing process in both healthy and alloxan-induced diabetic rats. Diabetes was induced with alloxan (100 mg/kg S. C). Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each orally and daily for 3 weeks. Under general anesthesia, full-thickness excisional skin wounds were created on the dorsal surface of thoracic region. Macroscopic and microscopic features of wound healing stages were recorded at weekly intervals and biochemical parameters were estimated at the end of 3 weeks. It was observed that as compared to control in the treated group there was early reappearance of epidermal and dermal components, reduced serum creatinine level, increased serum antioxidant status and total protein content and controlled glycemic status. It is concluded that oral co-administration of d-α-tocopherol and d-δ-TRF promotes skin wound healing in both healthy and diabetic rats through its antioxidant potency, therefore suggested that vitamin E isoforms hold promising future in the effective management of wounds in both otherwise healthy and diabetics.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Effect of Co-Administration of Vitamin E Isoforms  d-α-Tocopherol and d-δ-Tocotrienol Rich Fraction on the Healing of Skin Wounds in Diabetic Rats
    AU  - Bijo Elsy
    AU  - Aijaz Ahmed Khan
    AU  - Veena Maheshwari
    Y1  - 2017/10/31
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijbecs.20170305.11
    DO  - 10.11648/j.ijbecs.20170305.11
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
    SP  - 52
    EP  - 62
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20170305.11
    AB  - Normal wound healing involves sequence of events which is believed to be altered in diabetes due to hyperglycemia, infection and oxidative stress. The latter may be reduced by antioxidants which neutralize the chain formation of free radicals. Vitamin E is a well-known antioxidant and has saturated tocopherols and unsaturated tocotrienols. The most active form being the α-tocopherol. The present study was designed to explore the combined effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on wound healing process in both healthy and alloxan-induced diabetic rats. Diabetes was induced with alloxan (100 mg/kg S. C). Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each orally and daily for 3 weeks. Under general anesthesia, full-thickness excisional skin wounds were created on the dorsal surface of thoracic region. Macroscopic and microscopic features of wound healing stages were recorded at weekly intervals and biochemical parameters were estimated at the end of 3 weeks. It was observed that as compared to control in the treated group there was early reappearance of epidermal and dermal components, reduced serum creatinine level, increased serum antioxidant status and total protein content and controlled glycemic status. It is concluded that oral co-administration of d-α-tocopherol and d-δ-TRF promotes skin wound healing in both healthy and diabetic rats through its antioxidant potency, therefore suggested that vitamin E isoforms hold promising future in the effective management of wounds in both otherwise healthy and diabetics.
    VL  - 3
    IS  - 5
    ER  - 

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