The Influence of Biocatalytic Plant Extracts on Biogas Production from Kitchen Wastes at Cryo-mesophilic Temperature Regimes
International Journal of Economy, Energy and Environment
Volume 4, Issue 5, October 2019, Pages: 96-105
Received: Sep. 8, 2019; Accepted: Oct. 5, 2019; Published: Oct. 21, 2019
Views 475      Downloads 112
Bakari Chaka, Department of Mathematics and Physical Sciences, Maasai Mara University, Narok, Kenya
Aloys Osano, The Centre for Innovation, New and Renewable Energy (CINRE), Maasai Mara University, Narok, Kenya
Justin Maghanga, Department of Statistics and Physical Sciences, Taita Taveta University, Voi, Kenya
Martin Magu, Department of Chemistry, Multimedia University of Kenya, Nairobi, Kenya
Article Tools
Follow on us
Radicalization in waste-to-energy systems are on the rise to meet human energy demands. Biogas generation from kitchen wastes is one such scheme, though affected by poor yields and methane levels at low temperatures. In this research, biocatalytic extracts with fermentative properties were hereby assessed on their potential to fasten these processes and increase the biogas yield at ambient temperatures. The variations in kitchen waste substrate anaerobic parameters and elemental composition as well as biogas yields and methane levels were monitored in a 28-day retention period. Three 40-liter batch and unstirred bio-digesters containing biocatalysts Terminalia b., Acanthaceae spp. and a control setup were used. The results indicated rapid saccharification rates in the samples with additives. Terminalia b. additives exhibited high volatile solids hydrolysis rate of 98.3% followed by Acanthaceae spp. (50.8%) and control sample (29.4%). Similar trends were observed in organic carbon reduction as the levels of nitrogen, phosphorus and sulfur linearly increased. The biocatalysts did not affect substrate pH, volatile fatty acids and alkalinity levels. Terminalia b. sample produced 2.32 folds higher while Acanthaceae spp. sample produced 1.375 folds higher than the control sample. Terminalia b. methane levels were highest (45.475±0.922%) followed by the control sample (41.750±1.401) and Acanthaceae spp. sample (39.275±0.263%) after 28-day retention period at 19.5±0.5°C. Use of these biocatalysts in biofuel synthesis can thus optimize biogas production leading to greener economies.
Kitchen Waste, Biogas, Biocatalysts, Low Temperature
To cite this article
Bakari Chaka, Aloys Osano, Justin Maghanga, Martin Magu, The Influence of Biocatalytic Plant Extracts on Biogas Production from Kitchen Wastes at Cryo-mesophilic Temperature Regimes, International Journal of Economy, Energy and Environment. Vol. 4, No. 5, 2019, pp. 96-105. doi: 10.11648/j.ijeee.20190405.12
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Bušić, A., Kundas, S., Morzak, G., et al. Recent Trends in Biodiesel and Biogas Production. Food Technol Biotechnol. 2018; 56 (2): 152–173. doi: 10.17113/ftb.
Ciggin, A. Anaerobic co-digestion of sewage sludge with switchgrass: Experimental and kinetic evaluation. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects 2016; 38: 1, pages 15-21.
Greenough, W. The blue death Disease, disaster, and the water we drink. J Clin Invest. 2008; 118 (1): 4. doi: 10.1172/JCI34394.
Onyido, A., Okolo, P., Obiukwu, M. and Amadi, E. A Survey of Vectors of Public Health Diseases in Un-Disposed Refuse Dumps in Awka Town, Anambra State, South-eastern Nigeria. Research Journal of Parasitology, 2009; 4: 22-27.
Paritosh, K., Kushwaha, S., Yadav, M., Pareek, N., Chawade, A., Vivekanand, V. Food Waste to Energy: An Overview of Sustainable Approaches for Food Waste Management and Nutrient Recycling. Biomed Res Int. 2017; 2017: 2370927. doi: 10.1155/2017/2370927.
Xia, A., Cheng, J., Murphy, D. Innovation in biological production and upgrading of methane and hydrogen for use as gaseous transport biofuel. Biotechnol Adv (in press). 2016.
McKeown, M., Hughes, D., Collins, G., Mahony, T., O’Flaherty, V. Low-temperature anaerobic digestion for wastewater treatment. Curr Opin Biotechnol 2012; 23: 444–451.
Hwang, M., Jang, N., Hyum, S., Kim, I. “Anaerobic biohydrogen production from ethanol fermentation: the role of pH. J Biotechnol 2004; 111: 297–309.
Abdelgadir, A., Chen, X., Liu, J., Xie, X., Zhang, J., Zhang, K., Wang, H., Liu, N. Characteristics, process parameters, and inner components of anaerobic bioreactors. BioMed Res Int 2014: 1–10,
Van Lier, J., Sanz M., Lettinga, G. Effect of temperature on the anaerobic thermophilic conversion of volatile fatty acids by dispersed and granular sludge. Water Res 1996; 30: 199–207.
Abe, S., Chika, S., and Yuji, S. Testing Method of the Total Amount of Sulfur Content (as Sulfur Trioxide): A Collaborative Study, Research Report of Fertilizer, 2014; 7, p. 28-35.
Yasushi, S. Validation of Gravimetric Analysis for Determination of Sulfur Content (as Sulfur Trioxide) in Sulfur and its Compounds as Fertilizers, Research Report of Fertilizer, 2011; 4, 9-15.
Wagner, A., Malin, C., Lins, P., Gstraunthaler, G., & Illmer, P. Reactor performance of a 750 m3 anaerobic digestion plant: Varied substrate input conditions impacting methanogenic community. Anaerobe, 2014; 29, 29-33.
Ziels, R., Svensson, B., Sundberg, C., Larsson, M., Karlsson, A. & Yekta, S. Microbial rRNA gene expression and co-occurrence profiles associate with biokinetics and elemental composition in full-scale anaerobic digesters. Microbial Biotechnology, 2018; 11, 4, 694-709.
Jimenez, J., Latrille, E., Harmand, J., Robles, A., Ferrer, J., Gaida, D., Wolf, C;... Steyer, J. Instrumentation and control of anaerobic digestion processes: a review and some research challenges. Reviews in Environmental Science and Bio/technology, 2015; 14, 4, 615-648.
Yat, S., Berger, A., Shonnard, D. Kinetic characterization of dilute surface acid hydrolysis of timber varieties and switchgrass. Bioresour. Technol. 2008; 99, 3855–3863.
Gumisiriza, R., Hawumba, J., Okure, M., Hensel, O. Biomass waste-to-energy valorisation technologies: a review case for banana processing in Uganda. Biotechnology for Biofuels. 2017; 10 (1). 1754-6834.
Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y., Holtzapple, M., Ladisch, M. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 2005; 96, 673–686.
Wang, S., Ma, F., Ma, W., Wang, P., Zhao, G., Lu, X. Influence of Temperature on Biogas Production Efficiency and Microbial Community in a Two-Phase Anaerobic Digestion System. Water, 2019; 11, 133.
Al-Wabel, M., Hussain, Q., Usman, A., Ahmad, M., Abduljabbar, A., Sallam, A., & Ok, Y. Impact of biochar properties on soil conditions and agricultural sustainability: A review. Land Degradation & Development, 2018; 29, 7, 2124-2161.
Liu, Y. and Tay, J. State of the art of biogranulation technology for wastewater treatment. Biotechnol. Adv. 2004; 22: 533–563.
Klassen, V., Blifernez-Klassen, O., Hoekzema, Y., Mussgnug, J., & Kruse, O. A novel one-stage cultivation/fermentation strategy for improved biogas production with microalgal biomass. Journal of Biotechnology, 2015; 215, 44-51.
Lindner, J., Zielonka, S., Oechsner, H., & Lemmer, A. Effect of different pH-values on process parameters in two-phase anaerobic digestion of high-solid substrates. Environmental Technology, 2015; 36, 2, 198-207.
Ma, J., Zhao, Q.-B., Laurens, L., Jarvis, E., Nagle, N., Chen, S., & Frear, C.; Mechanism, kinetics and microbiology of inhibition caused by long-chain fatty acids in anaerobic digestion of algal biomass. Biotechnology for Biofuels, 2015; 8, 1.
Girotto, F.; Peng, W.; Rafieenia, R. & Cossu, R. Effect of Aeration Applied During Different Phases of Anaerobic Digestion. Waste and Biomass Valorization, 2018; 9, 2, 161-174.
Refai, S., Berger, S., Wassmann, K., Hecht, M., Dickhaus, T. & Deppenmeier, U. BEAP profiles as rapid test system for status analysis and early detection of process incidents in biogas plants. Journal of Industrial Microbiology & Biotechnology: Official Journal of the Society for Industrial Microbiology and Biotechnology, 2017; 44, 3, 465-476.
Yuan, H., and Zhu, N. Progress in inhibition mechanisms and process control of intermediates and by-products in sewage sludge anaerobic digestion. Renew Sustain Energ Rev 2016; 58: 429–438.
Gilbert, R.,Tomkins, N., Padmanabha, J., Gough, J., Krause, D. & McSweeney, C. Effect of finishing diets on Escherichia coli populations and prevalence of enterohaemorrhagic E. coli virulence genes in cattle faeces. Journal of Applied Microbiology, 2005; 99, 4, 885-894.
Vasconcelos, E., Leitão, R. & Santaella, S. Factors that affect bacterial ecology in hydrogen-producing anaerobic reactors. Bioenergy Research, 2016; 9, 4, 1260-1271.
Winde, L., Berghoff, A., Schories, G., & Mahro, B. Comparative evaluation of sludge surface charge as an indicator of process fluctuations in a biogas reactor. Engineering in Life Sciences, 2018; 18, 7, 484-491.
Zehnsdorf, A., Moeller, L., Stärk, H., Auge, H., Röhl, M. & Stinner, W. The study of the variability of biomass from plants of the Elodea genus from a river in Germany over a period of two hydrological years for investigating their suitability for biogas production. Energy, Sustainability and Society, 2017; 7, 1, 1-7.
Dykstra, C., & Pavlostathis, S. Evaluation of gas and carbon transport in a methanogenic bioelectrochemical system (BES). Biotechnology and Bioengineering, 2017; 114, 5, 961-969.
Ahmad, Z., Kai, H. and Harada, T. “Factors affecting immobilization and release of nitrogen in soil and chemical characteristics of the nitrogen newly immobilized II. Effect of carbon sources on immobilization and release of nitrogen in soil,” Soil Science and Plant Nutrition, 1969; 15, 6, pp. 252–258.
Chen, G., Zhao, G., Zhang, H., Shen, Y., Fei, H. & Cheng, W. Biogas slurry use as N fertilizer for two-season Zizania aquatica Turcz. in China. Nutrient Cycling in Agroecosystems: (formerly Fertilizer Research), 2017; 107, 3, 303-320.
Ezekoye, V., Ezekoye, B., and Offor, P. Effect of Retention Time on Biogas Production from Poultry Droppings and Cassava Peels. Nigerian Journal of Biotechnology, 2011; 22.
Yang, S., Tang, Y., Gou, M. & Jiang, X. Effect of sulfate addition on methane production and sulfate reduction in a mesophilic acetate-fed anaerobic reactor. Applied Microbiology and Biotechnology 2015; 99, 7, 3269-3277.
Krich, K., Augenstein, A., Batmale, J., Benemann, J., Rutledge, B. and Salour, D. “Upgrading dairy biogas to biomethane and other fuels,” in Biomethane from Dairy Waste-A Sourcebook for the Production and Use of Renewable Natural Gas in California, K. Andrews, Ed.,2005; pp. 47–69, Clear Concepts, California, Calif, USA.
Mahdy, A., Mendez, L., Tomás-Pejó, E., Del, M.; Ballesteros, M. & González-Fernández, C. Influence of enzymatic hydrolysis on the biochemical methane potential of Chlorella vulgaris and Scenedesmus sp. Journal of Chemical Technology & Biotechnology 2016; 91, 5, 1299-1305.
Lv, Z., Hu, M., Harms, H., Richnow, H., Liebetrau, J. and Nikolausz, M. Stable isotope composition of biogas allows early warning of complete process failure as a result of ammonia inhibition in anaerobic digesters. Bioresour. Technol. 2014; 167: 251–259. doi: 0.1016/j.biortech.2014.06.029.
Gaby, J., Zamanzadeh, M. and Horn, S. The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste. Biotechnol Biofuels. 2017; 10: 302. doi: 10.1186/s13068-017-0989-4.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186