European Journal of Clinical and Biomedical Sciences

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Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats

Received: 19 October 2016    Accepted: 11 November 2016    Published: 12 December 2016
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Abstract

Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.

DOI 10.11648/j.ejcbs.20160206.13
Published in European Journal of Clinical and Biomedical Sciences (Volume 2, Issue 6, December 2016)
Page(s) 69-82
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Irradiation, Oxidative Stress, AGE, Liver, Antioxidant Enzymes, Rats

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Author Information
  • Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

  • Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon

  • Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon

  • Faculty of Sciences, University of Dschang, Dschang, Cameroon

  • Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

  • National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon

  • Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

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    Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. (2016). Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. European Journal of Clinical and Biomedical Sciences, 2(6), 69-82. https://doi.org/10.11648/j.ejcbs.20160206.13

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    Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur. J. Clin. Biomed. Sci. 2016, 2(6), 69-82. doi: 10.11648/j.ejcbs.20160206.13

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    Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur J Clin Biomed Sci. 2016;2(6):69-82. doi: 10.11648/j.ejcbs.20160206.13

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  • @article{10.11648/j.ejcbs.20160206.13,
      author = {Kouam Foubi Brice Bertrand and Chuisseu Djamen Dieudonné Pascal and Dzeufiet Djomeni Paul Désiré and Samba Ngano Odette and Mouelle Sone and Tiedeu Alain Bertin and Gonsu Fotsin Joseph},
      title = {Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats},
      journal = {European Journal of Clinical and Biomedical Sciences},
      volume = {2},
      number = {6},
      pages = {69-82},
      doi = {10.11648/j.ejcbs.20160206.13},
      url = {https://doi.org/10.11648/j.ejcbs.20160206.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejcbs.20160206.13},
      abstract = {Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats
    AU  - Kouam Foubi Brice Bertrand
    AU  - Chuisseu Djamen Dieudonné Pascal
    AU  - Dzeufiet Djomeni Paul Désiré
    AU  - Samba Ngano Odette
    AU  - Mouelle Sone
    AU  - Tiedeu Alain Bertin
    AU  - Gonsu Fotsin Joseph
    Y1  - 2016/12/12
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ejcbs.20160206.13
    DO  - 10.11648/j.ejcbs.20160206.13
    T2  - European Journal of Clinical and Biomedical Sciences
    JF  - European Journal of Clinical and Biomedical Sciences
    JO  - European Journal of Clinical and Biomedical Sciences
    SP  - 69
    EP  - 82
    PB  - Science Publishing Group
    SN  - 2575-5005
    UR  - https://doi.org/10.11648/j.ejcbs.20160206.13
    AB  - Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.
    VL  - 2
    IS  - 6
    ER  - 

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