Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method
Journal of Photonic Materials and Technology
Volume 4, Issue 1, June 2018, Pages: 33-38
Received: Jan. 27, 2018; Accepted: Feb. 16, 2018; Published: Mar. 19, 2018
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Authors
Li Chen, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Baosheng Liu, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Chundan Wang, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Lihua Ma, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Hongcai Zhang, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Xu Guo, College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
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Abstract
The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.
Keywords
Cefepime, Papain, Reaction Mechanism, Spectroscopy, Determination
To cite this article
Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, Xu Guo, Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method, Journal of Photonic Materials and Technology. Vol. 4, No. 1, 2018, pp. 33-38. doi: 10.11648/j.jmpt.20180401.16
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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