American Journal of Nanosciences

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Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria

Received: 24 October 2019    Accepted: 14 November 2019    Published: 19 November 2019
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Abstract

This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.

DOI 10.11648/j.ajn.20190504.11
Published in American Journal of Nanosciences (Volume 5, Issue 4, December 2019)
Page(s) 27-38
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bioremediation, Nitrogen-fixing Bacteria, Phosphate-solubilizing Bacteria, Biofertilizer, Crude Oil Polluted Soil

References
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Author Information
  • Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria

  • Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria

  • Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria

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  • APA Style

    Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. (2019). Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. American Journal of Nanosciences, 5(4), 27-38. https://doi.org/10.11648/j.ajn.20190504.11

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    ACS Style

    Barivule Girigiri; Caroline Nchedu Ariole; Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am. J. Nanosci. 2019, 5(4), 27-38. doi: 10.11648/j.ajn.20190504.11

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    AMA Style

    Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am J Nanosci. 2019;5(4):27-38. doi: 10.11648/j.ajn.20190504.11

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  • @article{10.11648/j.ajn.20190504.11,
      author = {Barivule Girigiri and Caroline Nchedu Ariole and Herbert Okechukwu Stanley},
      title = {Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria},
      journal = {American Journal of Nanosciences},
      volume = {5},
      number = {4},
      pages = {27-38},
      doi = {10.11648/j.ajn.20190504.11},
      url = {https://doi.org/10.11648/j.ajn.20190504.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajn.20190504.11},
      abstract = {This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria
    AU  - Barivule Girigiri
    AU  - Caroline Nchedu Ariole
    AU  - Herbert Okechukwu Stanley
    Y1  - 2019/11/19
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajn.20190504.11
    DO  - 10.11648/j.ajn.20190504.11
    T2  - American Journal of Nanosciences
    JF  - American Journal of Nanosciences
    JO  - American Journal of Nanosciences
    SP  - 27
    EP  - 38
    PB  - Science Publishing Group
    SN  - 2575-4858
    UR  - https://doi.org/10.11648/j.ajn.20190504.11
    AB  - This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.
    VL  - 5
    IS  - 4
    ER  - 

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