The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris

doi:doi:10.11648/j.ijcda.20150104.12

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The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris

Published in International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 4)

Received: 7 February 2016 Accepted: 14 February 2016 Published: 26 February 2016

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Abstract

Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 4)
DOI	10.11648/j.ijcda.20150104.12
Page(s)	89-95
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Dimethylnitrosmine, Fibrosis, Liver, Rat, Cymbopogon Citratus

References

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APA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. (2016). The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. International Journal of Clinical and Developmental Anatomy, 1(4), 89-95. https://doi.org/10.11648/j.ijcda.20150104.12

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ACS Style

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int. J. Clin. Dev. Anat. 2016, 1(4), 89-95. doi: 10.11648/j.ijcda.20150104.12

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AMA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int J Clin Dev Anat. 2016;1(4):89-95. doi: 10.11648/j.ijcda.20150104.12

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@article{10.11648/j.ijcda.20150104.12,
  author = {Naglaa Ali Saber Sarg and Usama Fouad Ahmed and Ali Mohamed Ali and Saleh Sayed Idris},
  title = {The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {4},
  pages = {89-95},
  doi = {10.11648/j.ijcda.20150104.12},
  url = {https://doi.org/10.11648/j.ijcda.20150104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150104.12},
  abstract = {Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.},
 year = {2016}
}

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TY  - JOUR
T1  - The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats
AU  - Naglaa Ali Saber Sarg
AU  - Usama Fouad Ahmed
AU  - Ali Mohamed Ali
AU  - Saleh Sayed Idris
Y1  - 2016/02/26
PY  - 2016
N1  - https://doi.org/10.11648/j.ijcda.20150104.12
DO  - 10.11648/j.ijcda.20150104.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 89
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20150104.12
AB  - Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.
VL  - 1
IS  - 4
ER  -

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Author Information

Naglaa Ali Saber Sarg

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Usama Fouad Ahmed

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Ali Mohamed Ali

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Saleh Sayed Idris

Department of Health Education & Health Promotion, Umm Al-Qura University, Makkah City, Saudi Arabia

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Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. (2016). The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. International Journal of Clinical and Developmental Anatomy, 1(4), 89-95. https://doi.org/10.11648/j.ijcda.20150104.12

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ACS Style

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int. J. Clin. Dev. Anat. 2016, 1(4), 89-95. doi: 10.11648/j.ijcda.20150104.12

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AMA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int J Clin Dev Anat. 2016;1(4):89-95. doi: 10.11648/j.ijcda.20150104.12

Copy | Download

@article{10.11648/j.ijcda.20150104.12,
  author = {Naglaa Ali Saber Sarg and Usama Fouad Ahmed and Ali Mohamed Ali and Saleh Sayed Idris},
  title = {The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {4},
  pages = {89-95},
  doi = {10.11648/j.ijcda.20150104.12},
  url = {https://doi.org/10.11648/j.ijcda.20150104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150104.12},
  abstract = {Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats
AU  - Naglaa Ali Saber Sarg
AU  - Usama Fouad Ahmed
AU  - Ali Mohamed Ali
AU  - Saleh Sayed Idris
Y1  - 2016/02/26
PY  - 2016
N1  - https://doi.org/10.11648/j.ijcda.20150104.12
DO  - 10.11648/j.ijcda.20150104.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 89
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20150104.12
AB  - Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.
VL  - 1
IS  - 4
ER  -

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