Antioxidant Properties of Aqueous Extract of Salvadora persica in Rats Subjected to Forced Swimming Test
Biomedical Sciences
Volume 3, Issue 1, January 2017, Pages: 10-20
Received: Dec. 11, 2016; Accepted: Jan. 13, 2017; Published: Feb. 9, 2017
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Kholoud S. Ramadan, Department of Biochemistry, Faculty of Science-Al Faisaliah, King Abdulaziz University, Jeddah, Saudi Arabia
Hoda E. A. Farid, Department of Biochemistry, Faculty of Science-Al Faisaliah, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Biochemistry, Faculty of Agriculture, Minufiya University, Cairo, Egypt
Jummanah J., King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
Raya M. M. Almarashi, Department of Biochemistry, Faculty of Science-Al Faisaliah, King Abdulaziz University, Jeddah, Saudi Arabia
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Salvadora persica (SP) has been used as a medicinal purpose among global Muslim community. The present study was carried out to evaluate the antioxidant activity of aqueous extract of SP in male albino rats subjected to forced swimming test (FST) and related biochemical parameters. These activities were tested at dose of 900 mg/kg extracts of SP administered orally for 28 successive days. Rats were divided into two groups (12 animals/group): control group (distilled water) and extract group (SP aqueous extract at 900 mg/kg for two weeks. On day 14, animals will be further classified into four equal groups (six animals each): Undepressed control, depressed rats, extract plus undepressed rats and extract plus depressed for next two weeks. The weight of the animals will be measured every week. HPLC analysis revealed the profile of SP extract. It was found that extract significantly (p<0.001) increases mobility time in rats. It also showed significant (p<0.001) decreased in blood glucose and DNA fragmentation as compared to depressed group. Additionally, there was an imbalance of oxidants/antioxidants level in brain of depressed rats and a significant inhibition on serum butyrylcholinesterase (BuChE) activity in rats exposed to FST. The obtained results revealed that SP extract has got significant antioxidant activity. Hence, aqueous extract of SP may be explored further for the management of mental depression.
Salvadora persica, Depression, Force Swimming Test, DNA Fragmentation, Oxidative Stress
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Kholoud S. Ramadan, Hoda E. A. Farid, Jummanah J., Raya M. M. Almarashi, Antioxidant Properties of Aqueous Extract of Salvadora persica in Rats Subjected to Forced Swimming Test, Biomedical Sciences. Vol. 3, No. 1, 2017, pp. 10-20. doi: 10.11648/
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