Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis
Volume 3, Issue 6, November 2017, Pages: 114-118
Received: Oct. 19, 2017;
Accepted: Nov. 1, 2017;
Published: Dec. 5, 2017
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Rongkai Zhang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Guowei Li, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Dong Jiang, Department of Urology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Lukun Yang, Department of Anesthesiology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Dawei Zhang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Zhe Wang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Jun Liang, Department of Stomatology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
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Osteoarthritis (OA) is characterized by the degeneration of affected joints leading to pain and reduced mobility. Periostin appears as a potential candidate in transducing mechanical signals to stimulate chondrocyte proliferation. 15 rats (E-group) underwent both medial meniscectomy and medial collateral ligament (MCL) transaction, while the other 15 rats (S-group) underwent sham surgery. Animals were killed at 1, 2, and 4 weeks postsurgery, and 5 animals were put into use per-time point in each treatment group. Cartilage was harvested from tibial plateau and femoral condyle. We examined the level of Postn expression in cartilage of the experimental OA, using real-time PCR and immunohistochemistry analysis. The expression of Postn was about 2-fold higher at 1 week (P=0.08<0.05), 4-fold higher at 2 weeks (P=0.003<0.05) and 4-fold higher at 4 weeks (P=0.034<0.05) post-surgery in E-group compared to S-group. Postn protein expression levels were markedly increased in the E-Group compared with the S-Group at 1, 2 and 4 weeks post-surgery. This study will be of benefit to those subsequent studies using the OA model to evaluate the outcomes of Postn epxpression in mechanism of OA. Postn may play prominent roles in pathogenic mechanisms of OA.
Osteoarthritis (OA), Periostin (Postn), Cartilage, Rat Model of Osteoarthritis
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Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis, Biomedical Sciences.
Vol. 3, No. 6,
2017, pp. 114-118.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Steinberg J, Zeggini E: Functional genomics in osteoarthritis: Past, present and future. J Orthop Res 2016; 13: [Epub ahead of print].
Varady NH, Grodzinsky AJ: Osteoarthritis year in review 2015: mechanics. Osteoarthritis Cartilage 2016; 24:27-35.
Moore AC, Burris DL: Tribological and material properties for cartilage of and throughout the bovine stiﬂe: support for the altered joint kinematics hypothesis of osteoarthritis. Osteoarthritis Cartilage 2015; 23:161-9.
Wang H, Chen T, Torzilli P et al: Dynamic contact stress patterns on the tibial plateaus during simulated gait: a novel application of normalized cross correlation. J Biomech 2014; 47:568-74.
Horiuchi K, Amizuka N, Takeshita S et al: Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J. Bone Miner. Res 1999; 14: 1239-1249.
Rios H, Koushik SV, Wang H et al: periostin null mice exhibit dwarfism, incisor enamel defects, and an early-onset periodontal disease-like phenotype. Mol Cell Biol 2005; 25:11131-44.
Zhang R, Fang H, Chen Y et al: Gene Expression Analyses of Subchondral Bone in Early Experimental Osteoarthritis by Microarray. PLoS One 2012; 7: e32356.
Yucel I, Karaca E, Ozturan K, et al: Biomechanical and histological effects of intra-articular hyaluronic acid on anterior cruciate ligament in rats. Clin Biomech (Bristol, Avon) 2009; 24:571-6.
Botter SM1, Glasson SS, Hopkins B, et al: ADAMTS5L/L mice have less subchondral bone changes after induction of osteoarthritis through surgical instability: implications for a link between cartilage and subchondral bone changes. Osteoarthritis Cartilage 2009; 17:636-45.
Takayama G, Arima K, Kanaji T et al: Periostin: a novel component of subepithelial ﬁbrosis of bronchial asthma downstream of IL-4 and IL-13 signals. Allergy Clin Immunol 2006; 118:98–104.
Chijimatsu R, Kunugiza Y, Taniyama Y et al: Expression and pathological effects of periostin in human osteoarthritis cartilage. BMC Musculoskelet Disord 2015; 16:215.
Attur M, Yang Q, Shimada K et al: Elevated expression of periostin in human osteoarthritic cartilage and its potential role in matrix degradation via matrix metalloproteinase-13. FASEB J 2015; 29:4107-21.
Honsawek S, Wilairatana V, Udomsinprasert W et al: Association of plasma and synovial ﬂuid periostin with radiographic knee osteoarthritis: Cross-sectional study. Joint Bone Spine 2015; 82:352-5.
Rousseau JC, Sornay-Rendu E, Bertholon C et al: Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study. Osteoarthritis Cartilage 2015; 23:1736-42.
Buckwalter JA, Anderson DD, Brown TD et al: The roles of mechanical stresses in the pathogenesis of osteoarthritis: implications for treatment of joint injuries. Cartilage 2013; 4:286–94.
Koike M, Nojiri H, Ozawa Y et al: Mechanical overloading causes mitochondrial superoxide and SOD2 imbalance in chondrocytes resulting in cartilage degeneration. Sci Rep. 2015; 5:11722.
Madry H, van Dijk CN, Mueller-Gerbl M et al: The basic science of the subchondral bone. Knee Surg Sports Traumatol Arthrosc. 2010; 18:419-33.
Lajeunesse D1, Reboul P et al: Subchondral bone in osteoarthritis: a biologic link with articular cartilage leading to abnormal remodeling. Curr Opin Rheumatol. 2003; 15:628-33.