Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats

Zhumei Sun; Xu Zhao; Jianmin Li; Yaning Zhao; Changxiang Chen

doi:doi:10.11648/j.bs.20200603.14

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Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats

Zhumei Sun, Xu Zhao, Jianmin Li, Yaning Zhao, Changxiang Chen

Published in Biomedical Sciences (Volume 6, Issue 3)

Received: 5 August 2020 Accepted: 21 August 2020 Published: 10 September 2020

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Abstract

Background Acute ischemic stroke causes long-term neurological and neurobehavioral dysfunctions. With the development of clinical medicine, the importance of pre-ischemic exercise intervention has been gradually recognized, but its mechanism remains to be further explored. Objective This study investigates the effects of different exercise intensity preconditioning in changes of hippocampal neurons and the expression of Gadd45β and DNA-PKcs in the hippocampal region after cerebral ischemia-reperfusion in rats. Method 160 SD rats were divided into control group (n=40), cerebral ischemia reperfusion group (I/R group, n=40), middle intensity exercise preconditioning group (EI 1+I/R group, n=40), high intense exercise preconditioning group (EI 2+I/R group, n=40). Stroke was induced by improved Pulsinelli four blood vessel blocking after exercise preconditioning. Morphological changes of neurons in the hippocampal region of rats were observed by HE staining at 6 h, 1d, 3d and 7d after ischemia in each group. Immunohistochemistry method was used to detect the expression of Gadd45β and DNA-PKcs in hippocampus CA1. The mRNA level of Gadd45β and DNA-PKcs in hippocampal CA1 was detected by Real Time PCR. Results Compared with I/R group, the neuronal cell necrosis of was alleviated in EI 1+I/R group, but more serious in EI 2+I/R group; The expression of Gadd45β and DNA-PKcs were significantly higher in the EI 1+ I/R group, but lower in EI 2+I/R group (P<0.01). Conclusion Moderate intensity exercise preconditioning can improve the survival of neurons after cerebral ischemia-reperfusion injury in rats. However, high-intensity motor preconditioning increased the damage and loss of neurons, and its mechanism may be related to the regulation of the expression of Gadd45β and DNA-PKcs in the hippocampus of cerebral ischemia-reperfusion rats, thus protecting and promoting the function of DNA repair system.

Published in	Biomedical Sciences (Volume 6, Issue 3)
DOI	10.11648/j.bs.20200603.14
Page(s)	61-66
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Exercise, Cerebral Ischemia/Reperfusion, Gadd45β, DNA-PKcs

References

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[15]	Andrews SC, Curtin D, Hawi Z, et al. ntensity Matters: High-intensity Interval Exercise Enhances Motor Cortex Plasticity More Than Moderate Exercise [J]. Cereb Cortex. 2020 Jan 10; 30 (1): 101-112.
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APA Style

Zhumei Sun, Xu Zhao, Jianmin Li, Yaning Zhao, Changxiang Chen. (2020). Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomedical Sciences, 6(3), 61-66. https://doi.org/10.11648/j.bs.20200603.14

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ACS Style

Zhumei Sun; Xu Zhao; Jianmin Li; Yaning Zhao; Changxiang Chen. Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomed. Sci. 2020, 6(3), 61-66. doi: 10.11648/j.bs.20200603.14

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AMA Style

Zhumei Sun, Xu Zhao, Jianmin Li, Yaning Zhao, Changxiang Chen. Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomed Sci. 2020;6(3):61-66. doi: 10.11648/j.bs.20200603.14

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@article{10.11648/j.bs.20200603.14,
  author = {Zhumei Sun and Xu Zhao and Jianmin Li and Yaning Zhao and Changxiang Chen},
  title = {Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats},
  journal = {Biomedical Sciences},
  volume = {6},
  number = {3},
  pages = {61-66},
  doi = {10.11648/j.bs.20200603.14},
  url = {https://doi.org/10.11648/j.bs.20200603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20200603.14},
  abstract = {Background Acute ischemic stroke causes long-term neurological and neurobehavioral dysfunctions. With the development of clinical medicine, the importance of pre-ischemic exercise intervention has been gradually recognized, but its mechanism remains to be further explored. Objective This study investigates the effects of different exercise intensity preconditioning in changes of hippocampal neurons and the expression of Gadd45β and DNA-PKcs in the hippocampal region after cerebral ischemia-reperfusion in rats. Method 160 SD rats were divided into control group (n=40), cerebral ischemia reperfusion group (I/R group, n=40), middle intensity exercise preconditioning group (EI 1+I/R group, n=40), high intense exercise preconditioning group (EI 2+I/R group, n=40). Stroke was induced by improved Pulsinelli four blood vessel blocking after exercise preconditioning. Morphological changes of neurons in the hippocampal region of rats were observed by HE staining at 6 h, 1d, 3d and 7d after ischemia in each group. Immunohistochemistry method was used to detect the expression of Gadd45β and DNA-PKcs in hippocampus CA1. The mRNA level of Gadd45β and DNA-PKcs in hippocampal CA1 was detected by Real Time PCR. Results Compared with I/R group, the neuronal cell necrosis of was alleviated in EI 1+I/R group, but more serious in EI 2+I/R group; The expression of Gadd45β and DNA-PKcs were significantly higher in the EI 1+ I/R group, but lower in EI 2+I/R group (PConclusion Moderate intensity exercise preconditioning can improve the survival of neurons after cerebral ischemia-reperfusion injury in rats. However, high-intensity motor preconditioning increased the damage and loss of neurons, and its mechanism may be related to the regulation of the expression of Gadd45β and DNA-PKcs in the hippocampus of cerebral ischemia-reperfusion rats, thus protecting and promoting the function of DNA repair system.},
 year = {2020}
}

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TY  - JOUR
T1  - Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats
AU  - Zhumei Sun
AU  - Xu Zhao
AU  - Jianmin Li
AU  - Yaning Zhao
AU  - Changxiang Chen
Y1  - 2020/09/10
PY  - 2020
N1  - https://doi.org/10.11648/j.bs.20200603.14
DO  - 10.11648/j.bs.20200603.14
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 61
EP  - 66
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20200603.14
AB  - Background Acute ischemic stroke causes long-term neurological and neurobehavioral dysfunctions. With the development of clinical medicine, the importance of pre-ischemic exercise intervention has been gradually recognized, but its mechanism remains to be further explored. Objective This study investigates the effects of different exercise intensity preconditioning in changes of hippocampal neurons and the expression of Gadd45β and DNA-PKcs in the hippocampal region after cerebral ischemia-reperfusion in rats. Method 160 SD rats were divided into control group (n=40), cerebral ischemia reperfusion group (I/R group, n=40), middle intensity exercise preconditioning group (EI 1+I/R group, n=40), high intense exercise preconditioning group (EI 2+I/R group, n=40). Stroke was induced by improved Pulsinelli four blood vessel blocking after exercise preconditioning. Morphological changes of neurons in the hippocampal region of rats were observed by HE staining at 6 h, 1d, 3d and 7d after ischemia in each group. Immunohistochemistry method was used to detect the expression of Gadd45β and DNA-PKcs in hippocampus CA1. The mRNA level of Gadd45β and DNA-PKcs in hippocampal CA1 was detected by Real Time PCR. Results Compared with I/R group, the neuronal cell necrosis of was alleviated in EI 1+I/R group, but more serious in EI 2+I/R group; The expression of Gadd45β and DNA-PKcs were significantly higher in the EI 1+ I/R group, but lower in EI 2+I/R group (PConclusion Moderate intensity exercise preconditioning can improve the survival of neurons after cerebral ischemia-reperfusion injury in rats. However, high-intensity motor preconditioning increased the damage and loss of neurons, and its mechanism may be related to the regulation of the expression of Gadd45β and DNA-PKcs in the hippocampus of cerebral ischemia-reperfusion rats, thus protecting and promoting the function of DNA repair system.
VL  - 6
IS  - 3
ER  -

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Author Information

Zhumei Sun

Department of Clinical Medical, North China University of Science and Technology, Tangshan, China
Xu Zhao

Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan, China
Jianmin Li

Department of Clinical Medical, North China University of Science and Technology, Tangshan, China
Yaning Zhao

Department of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan, China
Changxiang Chen

Department of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan, China

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APA Style

Zhumei Sun, Xu Zhao, Jianmin Li, Yaning Zhao, Changxiang Chen. (2020). Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomedical Sciences, 6(3), 61-66. https://doi.org/10.11648/j.bs.20200603.14

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ACS Style

Zhumei Sun; Xu Zhao; Jianmin Li; Yaning Zhao; Changxiang Chen. Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomed. Sci. 2020, 6(3), 61-66. doi: 10.11648/j.bs.20200603.14

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AMA Style

Zhumei Sun, Xu Zhao, Jianmin Li, Yaning Zhao, Changxiang Chen. Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats. Biomed Sci. 2020;6(3):61-66. doi: 10.11648/j.bs.20200603.14

Copy | Download

@article{10.11648/j.bs.20200603.14,
  author = {Zhumei Sun and Xu Zhao and Jianmin Li and Yaning Zhao and Changxiang Chen},
  title = {Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats},
  journal = {Biomedical Sciences},
  volume = {6},
  number = {3},
  pages = {61-66},
  doi = {10.11648/j.bs.20200603.14},
  url = {https://doi.org/10.11648/j.bs.20200603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20200603.14},
  abstract = {Background Acute ischemic stroke causes long-term neurological and neurobehavioral dysfunctions. With the development of clinical medicine, the importance of pre-ischemic exercise intervention has been gradually recognized, but its mechanism remains to be further explored. Objective This study investigates the effects of different exercise intensity preconditioning in changes of hippocampal neurons and the expression of Gadd45β and DNA-PKcs in the hippocampal region after cerebral ischemia-reperfusion in rats. Method 160 SD rats were divided into control group (n=40), cerebral ischemia reperfusion group (I/R group, n=40), middle intensity exercise preconditioning group (EI 1+I/R group, n=40), high intense exercise preconditioning group (EI 2+I/R group, n=40). Stroke was induced by improved Pulsinelli four blood vessel blocking after exercise preconditioning. Morphological changes of neurons in the hippocampal region of rats were observed by HE staining at 6 h, 1d, 3d and 7d after ischemia in each group. Immunohistochemistry method was used to detect the expression of Gadd45β and DNA-PKcs in hippocampus CA1. The mRNA level of Gadd45β and DNA-PKcs in hippocampal CA1 was detected by Real Time PCR. Results Compared with I/R group, the neuronal cell necrosis of was alleviated in EI 1+I/R group, but more serious in EI 2+I/R group; The expression of Gadd45β and DNA-PKcs were significantly higher in the EI 1+ I/R group, but lower in EI 2+I/R group (PConclusion Moderate intensity exercise preconditioning can improve the survival of neurons after cerebral ischemia-reperfusion injury in rats. However, high-intensity motor preconditioning increased the damage and loss of neurons, and its mechanism may be related to the regulation of the expression of Gadd45β and DNA-PKcs in the hippocampus of cerebral ischemia-reperfusion rats, thus protecting and promoting the function of DNA repair system.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Effect of Different Exercise Intensity Preconditioning on Global Ischemia-Induced Neuronal Death and Expression of Gadd45β and DNA-PKcs in Rats
AU  - Zhumei Sun
AU  - Xu Zhao
AU  - Jianmin Li
AU  - Yaning Zhao
AU  - Changxiang Chen
Y1  - 2020/09/10
PY  - 2020
N1  - https://doi.org/10.11648/j.bs.20200603.14
DO  - 10.11648/j.bs.20200603.14
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 61
EP  - 66
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20200603.14
AB  - Background Acute ischemic stroke causes long-term neurological and neurobehavioral dysfunctions. With the development of clinical medicine, the importance of pre-ischemic exercise intervention has been gradually recognized, but its mechanism remains to be further explored. Objective This study investigates the effects of different exercise intensity preconditioning in changes of hippocampal neurons and the expression of Gadd45β and DNA-PKcs in the hippocampal region after cerebral ischemia-reperfusion in rats. Method 160 SD rats were divided into control group (n=40), cerebral ischemia reperfusion group (I/R group, n=40), middle intensity exercise preconditioning group (EI 1+I/R group, n=40), high intense exercise preconditioning group (EI 2+I/R group, n=40). Stroke was induced by improved Pulsinelli four blood vessel blocking after exercise preconditioning. Morphological changes of neurons in the hippocampal region of rats were observed by HE staining at 6 h, 1d, 3d and 7d after ischemia in each group. Immunohistochemistry method was used to detect the expression of Gadd45β and DNA-PKcs in hippocampus CA1. The mRNA level of Gadd45β and DNA-PKcs in hippocampal CA1 was detected by Real Time PCR. Results Compared with I/R group, the neuronal cell necrosis of was alleviated in EI 1+I/R group, but more serious in EI 2+I/R group; The expression of Gadd45β and DNA-PKcs were significantly higher in the EI 1+ I/R group, but lower in EI 2+I/R group (PConclusion Moderate intensity exercise preconditioning can improve the survival of neurons after cerebral ischemia-reperfusion injury in rats. However, high-intensity motor preconditioning increased the damage and loss of neurons, and its mechanism may be related to the regulation of the expression of Gadd45β and DNA-PKcs in the hippocampus of cerebral ischemia-reperfusion rats, thus protecting and promoting the function of DNA repair system.
VL  - 6
IS  - 3
ER  -

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