Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant <i>Pseudomonas</i> Wastewater Isolates

Elizabeth Omolola Oladapo; Fiona Stainsby; Mohammed Sani Abdulsalami; Enimie Endurance Oaikhena

doi:doi:10.11648/j.fem.20170302.12

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Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates

Elizabeth Omolola Oladapo, Fiona Stainsby, Mohammed Sani Abdulsalami, Enimie Endurance Oaikhena

Published in Frontiers in Environmental Microbiology (Volume 3, Issue 2)

Received: 28 February 2017 Accepted: 24 March 2017 Published: 30 May 2017

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Abstract

Silver get washed into sewerage systems and eventually to wastewater treatment plant (WWTP) due to its utilization in industries. This poses concerns about the toxicity of these particles to microorganisms which are involved in biodegradation of organic wastes in biological WWTP. Pseudomonas species (Biosensor cell A, B, C, D and E) originally isolated from WWTP and modified by incorporating a stable chromosomal copy of the lux operon (lux CDABE) derived from Escherichia coli S17ƛ pir were sensitive immediately upon addition of silver nanoparticles (AgNPs) and bulk silver in short terms of incubation ranging from 0 to 300minutes. Microtitre plate luminometre was used to generate detailed luminescence reduction data for the silver particles tested against the bacterial cells in various concentrations ranging from 9µg/ml to 2500µg/ml. The EC₅₀ values generated at various time points showed that the highest toxicity was observed at time point, 0 of incubation for both AgNPs and bulk silver (158µg/ml and 618µg/ml EC₅₀ values respectively); these EC₅₀ values also indicate that AgNPs are much more toxic than bulk silver. Two putative biosensors, E and D showed proportional responses of bioluminescence reduction with increasing toxicant concentrations up to 2500µg/ml, hence displaying dose-dependent responses, superior operational range and sensing capabilities; good features for toxicity assay. Therefore, the recombinant isolate can be used to assay the toxicity of silver particles.

Published in	Frontiers in Environmental Microbiology (Volume 3, Issue 2)
DOI	10.11648/j.fem.20170302.12
Page(s)	30-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pseudomonas Species, Recombinant, Bioluminescence, Biosensor Cells

References

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APA Style

Elizabeth Omolola Oladapo, Fiona Stainsby, Mohammed Sani Abdulsalami, Enimie Endurance Oaikhena. (2017). Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Frontiers in Environmental Microbiology, 3(2), 30-38. https://doi.org/10.11648/j.fem.20170302.12

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ACS Style

Elizabeth Omolola Oladapo; Fiona Stainsby; Mohammed Sani Abdulsalami; Enimie Endurance Oaikhena. Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Front. Environ. Microbiol. 2017, 3(2), 30-38. doi: 10.11648/j.fem.20170302.12

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AMA Style

Elizabeth Omolola Oladapo, Fiona Stainsby, Mohammed Sani Abdulsalami, Enimie Endurance Oaikhena. Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Front Environ Microbiol. 2017;3(2):30-38. doi: 10.11648/j.fem.20170302.12

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@article{10.11648/j.fem.20170302.12,
  author = {Elizabeth Omolola Oladapo and Fiona Stainsby and Mohammed Sani Abdulsalami and Enimie Endurance Oaikhena},
  title = {Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates},
  journal = {Frontiers in Environmental Microbiology},
  volume = {3},
  number = {2},
  pages = {30-38},
  doi = {10.11648/j.fem.20170302.12},
  url = {https://doi.org/10.11648/j.fem.20170302.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20170302.12},
  abstract = {Silver get washed into sewerage systems and eventually to wastewater treatment plant (WWTP) due to its utilization in industries. This poses concerns about the toxicity of these particles to microorganisms which are involved in biodegradation of organic wastes in biological WWTP. Pseudomonas species (Biosensor cell A, B, C, D and E) originally isolated from WWTP and modified by incorporating a stable chromosomal copy of the lux operon (lux CDABE) derived from Escherichia coli S17ƛ pir were sensitive immediately upon addition of silver nanoparticles (AgNPs) and bulk silver in short terms of incubation ranging from 0 to 300minutes. Microtitre plate luminometre was used to generate detailed luminescence reduction data for the silver particles tested against the bacterial cells in various concentrations ranging from 9µg/ml to 2500µg/ml. The EC50 values generated at various time points showed that the highest toxicity was observed at time point, 0 of incubation for both AgNPs and bulk silver (158µg/ml and 618µg/ml EC50 values respectively); these EC50 values also indicate that AgNPs are much more toxic than bulk silver. Two putative biosensors, E and D showed proportional responses of bioluminescence reduction with increasing toxicant concentrations up to 2500µg/ml, hence displaying dose-dependent responses, superior operational range and sensing capabilities; good features for toxicity assay. Therefore, the recombinant isolate can be used to assay the toxicity of silver particles.},
 year = {2017}
}

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TY  - JOUR
T1  - Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates
AU  - Elizabeth Omolola Oladapo
AU  - Fiona Stainsby
AU  - Mohammed Sani Abdulsalami
AU  - Enimie Endurance Oaikhena
Y1  - 2017/05/30
PY  - 2017
N1  - https://doi.org/10.11648/j.fem.20170302.12
DO  - 10.11648/j.fem.20170302.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 30
EP  - 38
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20170302.12
AB  - Silver get washed into sewerage systems and eventually to wastewater treatment plant (WWTP) due to its utilization in industries. This poses concerns about the toxicity of these particles to microorganisms which are involved in biodegradation of organic wastes in biological WWTP. Pseudomonas species (Biosensor cell A, B, C, D and E) originally isolated from WWTP and modified by incorporating a stable chromosomal copy of the lux operon (lux CDABE) derived from Escherichia coli S17ƛ pir were sensitive immediately upon addition of silver nanoparticles (AgNPs) and bulk silver in short terms of incubation ranging from 0 to 300minutes. Microtitre plate luminometre was used to generate detailed luminescence reduction data for the silver particles tested against the bacterial cells in various concentrations ranging from 9µg/ml to 2500µg/ml. The EC50 values generated at various time points showed that the highest toxicity was observed at time point, 0 of incubation for both AgNPs and bulk silver (158µg/ml and 618µg/ml EC50 values respectively); these EC50 values also indicate that AgNPs are much more toxic than bulk silver. Two putative biosensors, E and D showed proportional responses of bioluminescence reduction with increasing toxicant concentrations up to 2500µg/ml, hence displaying dose-dependent responses, superior operational range and sensing capabilities; good features for toxicity assay. Therefore, the recombinant isolate can be used to assay the toxicity of silver particles.
VL  - 3
IS  - 2
ER  -

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Author Information

Elizabeth Omolola Oladapo

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Fiona Stainsby

School of Life, Sport and Social Sciences, Edinburgh Napier University, Edinburgh, Scotland
Mohammed Sani Abdulsalami

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Enimie Endurance Oaikhena

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

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Elizabeth Omolola Oladapo, Fiona Stainsby, Mohammed Sani Abdulsalami, Enimie Endurance Oaikhena. (2017). Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Frontiers in Environmental Microbiology, 3(2), 30-38. https://doi.org/10.11648/j.fem.20170302.12

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ACS Style

Elizabeth Omolola Oladapo; Fiona Stainsby; Mohammed Sani Abdulsalami; Enimie Endurance Oaikhena. Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Front. Environ. Microbiol. 2017, 3(2), 30-38. doi: 10.11648/j.fem.20170302.12

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AMA Style

Elizabeth Omolola Oladapo, Fiona Stainsby, Mohammed Sani Abdulsalami, Enimie Endurance Oaikhena. Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates. Front Environ Microbiol. 2017;3(2):30-38. doi: 10.11648/j.fem.20170302.12

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@article{10.11648/j.fem.20170302.12,
  author = {Elizabeth Omolola Oladapo and Fiona Stainsby and Mohammed Sani Abdulsalami and Enimie Endurance Oaikhena},
  title = {Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates},
  journal = {Frontiers in Environmental Microbiology},
  volume = {3},
  number = {2},
  pages = {30-38},
  doi = {10.11648/j.fem.20170302.12},
  url = {https://doi.org/10.11648/j.fem.20170302.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20170302.12},
  abstract = {Silver get washed into sewerage systems and eventually to wastewater treatment plant (WWTP) due to its utilization in industries. This poses concerns about the toxicity of these particles to microorganisms which are involved in biodegradation of organic wastes in biological WWTP. Pseudomonas species (Biosensor cell A, B, C, D and E) originally isolated from WWTP and modified by incorporating a stable chromosomal copy of the lux operon (lux CDABE) derived from Escherichia coli S17ƛ pir were sensitive immediately upon addition of silver nanoparticles (AgNPs) and bulk silver in short terms of incubation ranging from 0 to 300minutes. Microtitre plate luminometre was used to generate detailed luminescence reduction data for the silver particles tested against the bacterial cells in various concentrations ranging from 9µg/ml to 2500µg/ml. The EC50 values generated at various time points showed that the highest toxicity was observed at time point, 0 of incubation for both AgNPs and bulk silver (158µg/ml and 618µg/ml EC50 values respectively); these EC50 values also indicate that AgNPs are much more toxic than bulk silver. Two putative biosensors, E and D showed proportional responses of bioluminescence reduction with increasing toxicant concentrations up to 2500µg/ml, hence displaying dose-dependent responses, superior operational range and sensing capabilities; good features for toxicity assay. Therefore, the recombinant isolate can be used to assay the toxicity of silver particles.},
 year = {2017}
}

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TY  - JOUR
T1  - Development of High-Throughput Methods for Nano- and Bulk Silver Toxicity Assays Using Bioluminescent Recombinant Pseudomonas Wastewater Isolates
AU  - Elizabeth Omolola Oladapo
AU  - Fiona Stainsby
AU  - Mohammed Sani Abdulsalami
AU  - Enimie Endurance Oaikhena
Y1  - 2017/05/30
PY  - 2017
N1  - https://doi.org/10.11648/j.fem.20170302.12
DO  - 10.11648/j.fem.20170302.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 30
EP  - 38
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20170302.12
AB  - Silver get washed into sewerage systems and eventually to wastewater treatment plant (WWTP) due to its utilization in industries. This poses concerns about the toxicity of these particles to microorganisms which are involved in biodegradation of organic wastes in biological WWTP. Pseudomonas species (Biosensor cell A, B, C, D and E) originally isolated from WWTP and modified by incorporating a stable chromosomal copy of the lux operon (lux CDABE) derived from Escherichia coli S17ƛ pir were sensitive immediately upon addition of silver nanoparticles (AgNPs) and bulk silver in short terms of incubation ranging from 0 to 300minutes. Microtitre plate luminometre was used to generate detailed luminescence reduction data for the silver particles tested against the bacterial cells in various concentrations ranging from 9µg/ml to 2500µg/ml. The EC50 values generated at various time points showed that the highest toxicity was observed at time point, 0 of incubation for both AgNPs and bulk silver (158µg/ml and 618µg/ml EC50 values respectively); these EC50 values also indicate that AgNPs are much more toxic than bulk silver. Two putative biosensors, E and D showed proportional responses of bioluminescence reduction with increasing toxicant concentrations up to 2500µg/ml, hence displaying dose-dependent responses, superior operational range and sensing capabilities; good features for toxicity assay. Therefore, the recombinant isolate can be used to assay the toxicity of silver particles.
VL  - 3
IS  - 2
ER  -

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