Detection of Multidrug Resistant and Shiga Toxin Producing <i>Escherichia coli</i> (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh

Jasmin Akter Runa; Mohammed Bakhtiar Lijon; Mohammed Abdur Rahman

doi:doi:10.11648/j.fem.20180401.13

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Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh

Jasmin Akter Runa, Mohammed Bakhtiar Lijon, Mohammed Abdur Rahman

Published in Frontiers in Environmental Microbiology (Volume 4, Issue 1)

Received: 26 December 2017 Accepted: 19 January 2018 Published: 2 February 2018

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Abstract

The research work was undertaken for detection and determination of antibiogram profile of Escherichia coli (E. coli ) that produce Shiga toxin from apparently healthy broilers (n=8) from different commercial farms of Jessore, Bangladesh. Broiler cloacal swabs (n=8) were collected by inserting the sterile swab stick in the cloacae of broiler and inoculated into peptone water for enrichment for 24 hours at 37°C. Enriched culture was streaked onto Eosin Methylene Blue (EMB) agar for isolation of pure bacterial culture. Then pure bacterial culture was streaked onto Mac-Conkey (MC) agar to identify Gram negative bacteria. Cultural method, Gram staining, biochemical reaction and polymerase chain reaction technique were used to identify the bacteria. The antibiogram profiles of bacteria were investigated against 6 commonly used antibiotics (Ciprofloxacin, Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin) by disc diffusion method. Five E. coli isolates were identified and Shiga toxin producing E. coli (STEC) was detected by amplifying 372-bp fragment of Stx2 gene in polymerase chain reaction (PCR) assay. The prevalence of the Shiga toxin generating E. coli (STEC) in broiler cloacal swab was 62.5%. All isolates (100%) were resistant to Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin and sensitive to Ciprofloxacin. The findings of this research strongly imply that broiler harbor multidrug resistant and Shiga toxin producing E. coli (STEC) which may cause public health problem if enter into human food chain.

Published in	Frontiers in Environmental Microbiology (Volume 4, Issue 1)
DOI	10.11648/j.fem.20180401.13
Page(s)	16-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antibiogram, Escherichia coli, PCR Assay, Prevalence, Shiga Toxin

References

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APA Style

Jasmin Akter Runa, Mohammed Bakhtiar Lijon, Mohammed Abdur Rahman. (2018). Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Frontiers in Environmental Microbiology, 4(1), 16-21. https://doi.org/10.11648/j.fem.20180401.13

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ACS Style

Jasmin Akter Runa; Mohammed Bakhtiar Lijon; Mohammed Abdur Rahman. Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Front. Environ. Microbiol. 2018, 4(1), 16-21. doi: 10.11648/j.fem.20180401.13

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AMA Style

Jasmin Akter Runa, Mohammed Bakhtiar Lijon, Mohammed Abdur Rahman. Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Front Environ Microbiol. 2018;4(1):16-21. doi: 10.11648/j.fem.20180401.13

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@article{10.11648/j.fem.20180401.13,
  author = {Jasmin Akter Runa and Mohammed Bakhtiar Lijon and Mohammed Abdur Rahman},
  title = {Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {1},
  pages = {16-21},
  doi = {10.11648/j.fem.20180401.13},
  url = {https://doi.org/10.11648/j.fem.20180401.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180401.13},
  abstract = {The research work was undertaken for detection and determination of antibiogram profile of Escherichia coli (E. coli ) that produce Shiga toxin from apparently healthy broilers (n=8) from different commercial farms of Jessore, Bangladesh. Broiler cloacal swabs (n=8) were collected by inserting the sterile swab stick in the cloacae of broiler and inoculated into peptone water for enrichment for 24 hours at 37°C. Enriched culture was streaked onto Eosin Methylene Blue (EMB) agar for isolation of pure bacterial culture. Then pure bacterial culture was streaked onto Mac-Conkey (MC) agar to identify Gram negative bacteria. Cultural method, Gram staining, biochemical reaction and polymerase chain reaction technique were used to identify the bacteria. The antibiogram profiles of bacteria were investigated against 6 commonly used antibiotics (Ciprofloxacin, Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin) by disc diffusion method. Five E. coli isolates were identified and Shiga toxin producing E. coli  (STEC) was detected by amplifying 372-bp fragment of Stx2 gene in polymerase chain reaction (PCR) assay. The prevalence of the Shiga toxin generating E. coli  (STEC) in broiler cloacal swab was 62.5%. All isolates (100%) were resistant to Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin and sensitive to Ciprofloxacin. The findings of this research strongly imply that broiler harbor multidrug resistant and Shiga toxin producing E. coli  (STEC) which may cause public health problem if enter into human food chain.},
 year = {2018}
}

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TY - JOUR
T1 - Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh
AU - Jasmin Akter Runa
AU - Mohammed Bakhtiar Lijon
AU - Mohammed Abdur Rahman
Y1 - 2018/02/02
PY - 2018
N1 - https://doi.org/10.11648/j.fem.20180401.13
DO - 10.11648/j.fem.20180401.13
T2 - Frontiers in Environmental Microbiology
JF - Frontiers in Environmental Microbiology
JO - Frontiers in Environmental Microbiology
SP - 16
EP - 21
PB - Science Publishing Group
SN - 2469-8067
UR - https://doi.org/10.11648/j.fem.20180401.13
AB - The research work was undertaken for detection and determination of antibiogram profile of Escherichia coli (E. coli ) that produce Shiga toxin from apparently healthy broilers (n=8) from different commercial farms of Jessore, Bangladesh. Broiler cloacal swabs (n=8) were collected by inserting the sterile swab stick in the cloacae of broiler and inoculated into peptone water for enrichment for 24 hours at 37°C. Enriched culture was streaked onto Eosin Methylene Blue (EMB) agar for isolation of pure bacterial culture. Then pure bacterial culture was streaked onto Mac-Conkey (MC) agar to identify Gram negative bacteria. Cultural method, Gram staining, biochemical reaction and polymerase chain reaction technique were used to identify the bacteria. The antibiogram profiles of bacteria were investigated against 6 commonly used antibiotics (Ciprofloxacin, Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin) by disc diffusion method. Five E. coli isolates were identified and Shiga toxin producing E. coli (STEC) was detected by amplifying 372-bp fragment of Stx2 gene in polymerase chain reaction (PCR) assay. The prevalence of the Shiga toxin generating E. coli (STEC) in broiler cloacal swab was 62.5%. All isolates (100%) were resistant to Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin and sensitive to Ciprofloxacin. The findings of this research strongly imply that broiler harbor multidrug resistant and Shiga toxin producing E. coli (STEC) which may cause public health problem if enter into human food chain.
VL - 4
IS - 1
ER -

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Author Information

Jasmin Akter Runa

Department of Microbiology, Jessore Science and Technology University, Jessore, Bangladesh
Mohammed Bakhtiar Lijon

Microbiology Laboratory, Modern Food Testing Laboratory, Chittagong City, Bangladesh
Mohammed Abdur Rahman

Microbiology Laboratory, Modern Food Testing Laboratory, Chittagong City, Bangladesh

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APA Style

Jasmin Akter Runa, Mohammed Bakhtiar Lijon, Mohammed Abdur Rahman. (2018). Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Frontiers in Environmental Microbiology, 4(1), 16-21. https://doi.org/10.11648/j.fem.20180401.13

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ACS Style

Jasmin Akter Runa; Mohammed Bakhtiar Lijon; Mohammed Abdur Rahman. Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Front. Environ. Microbiol. 2018, 4(1), 16-21. doi: 10.11648/j.fem.20180401.13

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AMA Style

Jasmin Akter Runa, Mohammed Bakhtiar Lijon, Mohammed Abdur Rahman. Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh. Front Environ Microbiol. 2018;4(1):16-21. doi: 10.11648/j.fem.20180401.13

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@article{10.11648/j.fem.20180401.13,
  author = {Jasmin Akter Runa and Mohammed Bakhtiar Lijon and Mohammed Abdur Rahman},
  title = {Detection of Multidrug Resistant and Shiga Toxin Producing Escherichia coli (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {1},
  pages = {16-21},
  doi = {10.11648/j.fem.20180401.13},
  url = {https://doi.org/10.11648/j.fem.20180401.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180401.13},
  abstract = {The research work was undertaken for detection and determination of antibiogram profile of Escherichia coli (E. coli ) that produce Shiga toxin from apparently healthy broilers (n=8) from different commercial farms of Jessore, Bangladesh. Broiler cloacal swabs (n=8) were collected by inserting the sterile swab stick in the cloacae of broiler and inoculated into peptone water for enrichment for 24 hours at 37°C. Enriched culture was streaked onto Eosin Methylene Blue (EMB) agar for isolation of pure bacterial culture. Then pure bacterial culture was streaked onto Mac-Conkey (MC) agar to identify Gram negative bacteria. Cultural method, Gram staining, biochemical reaction and polymerase chain reaction technique were used to identify the bacteria. The antibiogram profiles of bacteria were investigated against 6 commonly used antibiotics (Ciprofloxacin, Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin) by disc diffusion method. Five E. coli isolates were identified and Shiga toxin producing E. coli  (STEC) was detected by amplifying 372-bp fragment of Stx2 gene in polymerase chain reaction (PCR) assay. The prevalence of the Shiga toxin generating E. coli  (STEC) in broiler cloacal swab was 62.5%. All isolates (100%) were resistant to Ampicillin, Colistinsulphate, Erythromycin, Neomycin and Penicillin and sensitive to Ciprofloxacin. The findings of this research strongly imply that broiler harbor multidrug resistant and Shiga toxin producing E. coli  (STEC) which may cause public health problem if enter into human food chain.},
 year = {2018}
}

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